Align ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate GFF3785 Psest_3854 ABC-type branched-chain amino acid transport systems, ATPase component
Query= uniprot:Q1MCU3 (247 letters) >FitnessBrowser__psRCH2:GFF3785 Length = 234 Score = 164 bits (416), Expect = 1e-45 Identities = 93/214 (43%), Positives = 134/214 (62%), Gaps = 4/214 (1%) Query: 9 QPLLQVNGVETYYGNIRALAGVDVHVNKGEIVSLIGANGAGKSTLMMTICGSPQARTGSV 68 +PLL V + +YG AL G+D+ V+ GE V+L+G NG GK+T + I G + R+GS+ Sbjct: 3 EPLLSVRDLNAWYGESHALHGIDLDVHAGETVTLLGRNGVGKTTALRAIVGIIRKRSGSI 62 Query: 69 VFEGRDITRMPTHEIARLRIAQSPEGRRIFPRMTVLENLQMGAGLDNLKHFAEDVEKIFT 128 F+G+D+ R+P H AR I PE R IF +TV ENL + + + +I+ Sbjct: 63 RFDGKDMLRVPLHRTARHGIGYVPEERGIFSTLTVEENLTLP---PVVAKGGMTLAEIYQ 119 Query: 129 LFPRLKERHAQRGGTLSGGEQQMLSIGRALMARPKLLLLDEPSLGLAPLIVKGIFEAIRK 188 LFP L+ER G LSGGEQQML++ R L KLLLLDEP+ GLAP+I++ I E ++ Sbjct: 120 LFPNLEERRKSPGTKLSGGEQQMLAMARILRTGAKLLLLDEPTEGLAPVIIQRIGEVLQT 179 Query: 189 LNEAEGLTVFLVEQNAFAALRLSHRAYVMVNGKV 222 L + G+T+ LVEQN A +++ R YV+ +G+V Sbjct: 180 LKQ-RGMTILLVEQNFRFASKVADRFYVVDHGQV 212 Lambda K H 0.320 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 173 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 234 Length adjustment: 23 Effective length of query: 224 Effective length of database: 211 Effective search space: 47264 Effective search space used: 47264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory