GapMind for catabolism of small carbon sources

 

Aligments for a candidate for natD in Pseudomonas stutzeri RCH2

Align NatD aka LivH aka SLR0949, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized)
to candidate GFF1278 Psest_1311 Branched-chain amino acid ABC-type transport system, permease components

Query= TCDB::P74318
         (286 letters)



>lcl|FitnessBrowser__psRCH2:GFF1278 Psest_1311 Branched-chain amino
           acid ABC-type transport system, permease components
          Length = 307

 Score =  145 bits (365), Expect = 1e-39
 Identities = 87/294 (29%), Positives = 161/294 (54%), Gaps = 21/294 (7%)

Query: 5   QLIFNGIAVGSIIALGAVGLTLTYGILRLSNFAHGDFMTLAAYLTWWANTSGINLWL-SM 63
           Q + NG+ VGS  AL A+G T+ YGI+ + NFAHG+   + +Y+T+ A      + L ++
Sbjct: 9   QQLINGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVTFIAIVGLSMMGLDAL 68

Query: 64  ALGCVGTIIAMFIG--------EWLLWKPMRARRATATTLIIISIGLALFLRNGILLIWG 115
            +  +G  +A  I         E + ++P+R         +I +IG+++FL+N +LL   
Sbjct: 69  PILMIGAFVAAMIVTSAYGYSIERVAYRPLRGSNRLIP--LISAIGMSIFLQNVVLL--A 124

Query: 116 GNNQNYRVP-------IVPAQDFMGIKFEYYRLLVIAMAIAAMVVLHLILQRTKVGKAMR 168
            ++++  +P       ++      G+   Y ++L+  +   AM  L L + R+++G+A R
Sbjct: 125 QDSKDKAIPNLMPGNLVIGESAMNGVVISYMQILIFVVTFVAMYGLTLFISRSRLGRACR 184

Query: 169 AVADNVDLAKVSGINVEWVVMWTWVMTAVLTALGGSMYGLMT-TLKPNMGWFLILPMFAS 227
           A A+++ +A + GIN   ++  T+V+ A L A+   +  +    + P++G+   +  F +
Sbjct: 185 ACAEDLKMANLLGINTNSIIALTFVIGAALAAVAAVLISMQYGVINPHIGFLAGIKAFTA 244

Query: 228 VILGGIGNPYGAIAGGIIIGVAQEVSVPWFGTSYKMGVALLLMIIILFIRPQGL 281
            +LGGIG+  GA+ GG+++GVA+      FG  YK  VA  L+I++L  RP G+
Sbjct: 245 AVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFSLLILVLLFRPTGI 298


Lambda     K      H
   0.329    0.143    0.450 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 183
Number of extensions: 11
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 286
Length of database: 307
Length adjustment: 26
Effective length of query: 260
Effective length of database: 281
Effective search space:    73060
Effective search space used:    73060
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory