GapMind for catabolism of small carbon sources

 

Aligments for a candidate for ech in Pseudomonas stutzeri RCH2

Align enoyl-CoA hydratase (EC 4.2.1.17) (characterized)
to candidate GFF3899 Psest_3969 3-hydroxyacyl-CoA dehydrogenase

Query= BRENDA::P07896
         (722 letters)



>lcl|FitnessBrowser__psRCH2:GFF3899 Psest_3969 3-hydroxyacyl-CoA
           dehydrogenase
          Length = 412

 Score =  296 bits (757), Expect = 2e-84
 Identities = 154/385 (40%), Positives = 232/385 (60%), Gaps = 16/385 (4%)

Query: 296 VSSVGVLGLGTMGRGIAISFARVGISVVAVESDPKQLDAAKKII--TFTLEKEASRAHQN 353
           + +  V+G GTMGRGI +S A  G+ V  ++++P+ L+ A   +  T+       R  + 
Sbjct: 8   IQTAAVIGAGTMGRGIVMSLANAGVQVRWLDNNPEMLERALGTVADTYAHNVRQGRIDEA 67

Query: 354 GQASAKPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVFAELSALCKPGAFLCTNTSALN 413
             A+ +  +  ++  + L+ VDLV+EAV+E++ LK+K+F EL  + KP   L +NTSAL+
Sbjct: 68  QAAARRACISKAADYQALADVDLVIEAVYENLELKQKIFRELDGIVKPAGILASNTSALD 127

Query: 414 VDDIASSTDRPQLVIGTHFFSPAHVMRLLEVIPSRYSSPTTIATVMSLSKKIGKIGVVVG 473
           +D IA+ T RP+ V+G HFFSPAH+M+LLE++    +S   +     L K++GK+ V+ G
Sbjct: 128 LDAIAAVTKRPEQVVGLHFFSPAHIMKLLEIVRGAKTSKAVLDASTELGKRMGKVSVIAG 187

Query: 474 NCYGFVGNRMLAPYYNQGFFLLEEGSKPEDVDGVLEEFGFKMGPFRVSDLAGLDVGWKIR 533
           NC GF+GNRMLA Y  +   +L EG+ P  VD  L+ FGF MGPFR+ D+ G+D+ W+ R
Sbjct: 188 NCQGFIGNRMLATYVREARMMLLEGAYPHQVDAALQGFGFAMGPFRMYDVVGIDLEWRAR 247

Query: 534 KGQGLTGPSLPPGTPVRKRGNSRYSPLGDMLCEAGRFGQKTGKGWYQYDKPLGRIHKPDP 593
           +  G             K  +     + + LCE GRFGQK+  G+Y+Y +   R  + DP
Sbjct: 248 ELAG-------------KGQDEVEVQVDNRLCELGRFGQKSRMGYYRYAEG-SRQAEHDP 293

Query: 594 WLSTFLSQYREVHHIEQRTISKEEILERCLYSLINEAFRILEEGMAARPEHIDVIYLHGY 653
            +   + +  E    ++R I  EEILERCL +L+NE  +ILEEGMA     ID +YL+GY
Sbjct: 294 EVDALVQRESERLGFQRREIGSEEILERCLLALVNEGAKILEEGMAESSADIDTVYLYGY 353

Query: 654 GWPRHKGGPMFYAASVGLPTVLEKL 678
           G+P   GGPM +A   GLP + ++L
Sbjct: 354 GFPAEVGGPMTWADRQGLPAIRDRL 378


Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 676
Number of extensions: 32
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 722
Length of database: 412
Length adjustment: 35
Effective length of query: 687
Effective length of database: 377
Effective search space:   258999
Effective search space used:   258999
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory