Align Exo-alpha-(1->6)-L-arabinopyranosidase; APY; Beta-D-galactopyranosidase; EC 3.2.1.- (characterized)
to candidate GFF1538 Psest_1575 Beta-glucosidase-related glycosidases
Query= SwissProt::E7CY69 (757 letters) >FitnessBrowser__psRCH2:GFF1538 Length = 763 Score = 241 bits (615), Expect = 1e-67 Identities = 209/685 (30%), Positives = 313/685 (45%), Gaps = 83/685 (12%) Query: 36 GIPSYMITDGPHGLRKSLASSAGETDLDDSVPATCFPPAAGLSSSWNPELIHKVGEAMAE 95 GIP + D HG R T FP + L+SSW+ E I G A Sbjct: 98 GIPIFFAYDVIHGHR------------------TIFPISLALASSWDLEAIALSGRVSAI 139 Query: 96 ECIQEKVAVILGPGVNIKRNPLGGRCFEYWSEDPYLAGHEAIGIVEGVQSK------GVG 149 E + + + P V+I R+P GR E + EDPYL A +V Q + V Sbjct: 140 EASADGLDLTFAPMVDITRDPRWGRTSEGFGEDPYLVSQIAGTLVRAYQGERLSAADSVM 199 Query: 150 TSLKHFAANNQ-ETDRLRVDARISPRALREIYFPAFEHIVKKAQPWTIMCSYNRINGVHS 208 S+KHFA E R +SP+ + + Y P + V A +M + N +NG+ + Sbjct: 200 ASVKHFALYGAVEGGRDYNVVDMSPQRMHQDYLPPYRAAVD-AGAGGVMVALNTVNGMPA 258 Query: 209 AQNHWLLTDVLRDEWGFDGIVMSDWGA-----------DHDRGA--SLNAGLNLEMPPSY 255 + N WLL D+LRD+WGF G+ +SD GA D A ++ AG++L M S Sbjct: 259 SANRWLLRDLLRDDWGFRGLNISDHGAIDELLRHGVARDGREAARLAIEAGIDLSMHDSL 318 Query: 256 TDDQIVYAVRDGLITPAQLDRMAQGMIDLVNKTRAAMSIDNYR---------FDVDA--- 303 ++ V G + P +L A G + L K + D YR +V+A Sbjct: 319 YLQELPGLVERGEV-PIELIDQAVGRV-LGAKYDLGLFHDPYRRIGQAADDPVEVNAESR 376 Query: 304 -HDEVAHQAAIESIVMLKNDDAILPLN-------AGPVA----------NPSATPQKIAV 345 H + A + A +S+V+L+N + LPL GP+A + + + Sbjct: 377 LHRQAARRVARDSLVLLENPEQTLPLRKDATIALVGPLADSHVDMLGSWSAAGVAAQTVT 436 Query: 346 IGEFARTPRYQGGGSSHITPTKMTSFLDTLAERGIKADFAPGFTLDLEPADP-ALESEAV 404 + + +GG H +T + P +LD P P A+ EAV Sbjct: 437 LRQGLEAAVGKGGQIIHARGASVTDDAGMIKYLNFLNWDRPEVSLD--PRTPQAMIDEAV 494 Query: 405 ETAKNADVVLMFLGLPEAVESEGFDRDTLDMPAKQIALLEQVAAANQNVVVVLSNGSVIT 464 A+ ADV++ +G + E R +L +PA Q ALLE + A + +VVVL NG + Sbjct: 495 RAAREADVIVAAVGEARGMSHESSSRTSLALPASQQALLEALVATGKPLVVVLMNGRPLQ 554 Query: 465 VAPWAKNAKGILESWLLGQSGGPALADVIFGQVSPSGKLAQSIPLDINDDPSMLNWPGEE 524 + ++A +LE+W G GG A+ADV+FG +PSGKL S P + P+ N P Sbjct: 555 LGWVKEHADAVLETWFAGTEGGHAIADVLFGAHNPSGKLPISFPRSVGQIPTYYNHP-RL 613 Query: 525 GHVDYGEGVFAGY--RYYDTYGKAVDYPFGYGLSYATFEITGVAVAKTGANTA---TVTA 579 G Y EG Y +Y++ A+ YPFGYGLSY FE++ +++ N V+ Sbjct: 614 GR-PYVEGRPGNYTSQYFEEPNGAL-YPFGYGLSYTDFELSKPQLSRRTLNRGQKLEVSV 671 Query: 580 TVTNTSDVDAAETVQVYVVPGKADVARPKHELKGFTKAFLKAGESKTVAIDLDERAFAYW 639 TV NT A VQ+Y+ RP ELKGFTK L+ GE++ V L E ++ Sbjct: 672 TVKNTGARAGATVVQLYLQDLVGSSVRPVKELKGFTKLMLEPGETQLVRFVLGEEDLKFY 731 Query: 640 SEKYNDWHVEAGEYAIEVGVSSRDI 664 + D E GE+ +++G+ S+ + Sbjct: 732 DARL-DHVAEPGEFEVQLGLDSQSV 755 Lambda K H 0.315 0.132 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1288 Number of extensions: 60 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 757 Length of database: 763 Length adjustment: 40 Effective length of query: 717 Effective length of database: 723 Effective search space: 518391 Effective search space used: 518391 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory