Align triose-phosphate isomerase (EC 5.3.1.1) (characterized)
to candidate GFF946 Psest_0975 triosephosphate isomerase
Query= BRENDA::P0A858 (255 letters) >FitnessBrowser__psRCH2:GFF946 Length = 251 Score = 235 bits (600), Expect = 6e-67 Identities = 123/250 (49%), Positives = 165/250 (66%), Gaps = 2/250 (0%) Query: 1 MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPEMYIDMAKREAEGSHIM 60 MR P+V GNWK+NG+R V ELV +LR + V+ +A+ P +++ + H+ Sbjct: 1 MRRPMVAGNWKMNGTRASVAELVESLRMQALPVS-VEIAVFPSCLHVAQVLGGVDSKHVA 59 Query: 61 LGAQNVDL-NLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYHKESDELIAKKFAVLKEQ 119 +GAQ+ N GA TGE SA ++G +++++GHSERR E+DE++++KF + Sbjct: 60 VGAQDCAAQNGFGALTGEVSAEQFAEVGCEWVLVGHSERRLVLGETDEVVSRKFLAAQVG 119 Query: 120 GLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFEGAVIAYEPVWAIGTGKSAT 179 GL PVLC+GET E EAG+T EV RQ+ VL G A FE AVIAYEPVWAIG+G +AT Sbjct: 120 GLKPVLCLGETLEEREAGRTLEVVGRQLAQVLDDHGIAVFESAVIAYEPVWAIGSGLTAT 179 Query: 180 PAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNAAELFAQPDIDGALVGGASLKA 239 P QAQ VH IR+ +++ D +A V + YGGSV A NAAELF+ DIDG LVGGASLKA Sbjct: 180 PEQAQEVHAAIREQLSRQDRQVAAGVRLLYGGSVKADNAAELFSMADIDGGLVGGASLKA 239 Query: 240 DAFAVIVKAA 249 + F I +AA Sbjct: 240 NEFGAICRAA 249 Lambda K H 0.316 0.130 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 180 Number of extensions: 8 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 255 Length of database: 251 Length adjustment: 24 Effective length of query: 231 Effective length of database: 227 Effective search space: 52437 Effective search space used: 52437 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 46 (22.3 bits)
Align candidate GFF946 Psest_0975 (triosephosphate isomerase)
to HMM TIGR00419 (tpiA: triose-phosphate isomerase (EC 5.3.1.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00419.hmm # target sequence database: /tmp/gapView.27483.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00419 [M=228] Accession: TIGR00419 Description: tim: triose-phosphate isomerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-68 216.6 2.3 2.4e-68 216.4 2.3 1.0 1 lcl|FitnessBrowser__psRCH2:GFF946 Psest_0975 triosephosphate isome Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__psRCH2:GFF946 Psest_0975 triosephosphate isomerase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 216.4 2.3 2.4e-68 2.4e-68 1 228 [] 5 240 .. 5 240 .. 0.92 Alignments for each domain: == domain 1 score: 216.4 bits; conditional E-value: 2.4e-68 TIGR00419 1 lviinfKlnesvgkvelevaklaeevaseagvevavappfvdldvvkdeve.seiqvaAqnvdavk.sGaftGeis 74 +v +n+K+n+++ +v+++v +l+ + a + ve+av p+ +++ v v+ ++ v+Aq++ a++ +Ga tGe+s lcl|FitnessBrowser__psRCH2:GFF946 5 MVAGNWKMNGTRASVAELVESLRMQ-ALPVSVEIAVFPSCLHVAQVLGGVDsKHVAVGAQDCAAQNgFGALTGEVS 79 699********************98.778899******************9789********986537******** PP TIGR00419 75 AemlkdlGakgvligHsErRsllkeadeliekkvarlkelglksvvCvgetleereaartinnvattaaaaA.... 146 Ae +++G+++vl+gHsErR +l e+de++ k+ ++ glk+v+C+getleerea+rt+++v ++ a + lcl|FitnessBrowser__psRCH2:GFF946 80 AEQFAEVGCEWVLVGHSERRLVLGETDEVVSRKFLAAQVGGLKPVLCLGETLEEREAGRTLEVVGRQLAQVLddhg 155 ********************************************************66666666555443333333 PP TIGR00419 147 ...lepdvvAvEPveliGtGkpvskAeaevveksvrdhlkkvskevaesvrvlyGasvtaaedaelaaqldvdGvL 219 +e++v+A+EPv++iG+G ++++ +a++v++ +r+ l++ +++va vr+lyG+sv+a ++ael+ d+dG L lcl|FitnessBrowser__psRCH2:GFF946 156 iavFESAVIAYEPVWAIGSGLTATPEQAQEVHAAIREQLSRQDRQVAAGVRLLYGGSVKADNAAELFSMADIDGGL 231 334************************************************************************* PP TIGR00419 220 lasavlkae 228 +++a+lka+ lcl|FitnessBrowser__psRCH2:GFF946 232 VGGASLKAN 240 *******95 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (228 nodes) Target sequences: 1 (251 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 5.88 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory