Alignments for a candidate for cadA in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for cadA in Pseudomonas stutzeri RCH2

Align lysine decarboxylase (EC 4.1.1.18) (characterized)
to candidate GFF635 Psest_0649 Glutamate decarboxylase and related PLP-dependent proteins

Query= BRENDA::Q9L072
         (480 letters)



>FitnessBrowser__psRCH2:GFF635
          Length = 507

 Score =  538 bits (1385), Expect = e-157
 Identities = 283/484 (58%), Positives = 342/484 (70%), Gaps = 16/484 (3%)

Query: 5   LLNDTTAEQYRRSVTEGVERVAAKLATTDRPFTGVTVDALSPRIDAIDLDEPLHDTAAVL 64
           L   +  + YR+ + +G+  V   L    +PF+G+    L+   + +DL+ PL D  A L
Sbjct: 19  LFTRSNHDDYRQLLQQGLNLVQQCLQRQQQPFSGILPQELAADFERVDLERPLGDAGAAL 78

Query: 65  DELEDVYLRDAVYFHHPRYLAHLNCPVVIPALLGEAVLSAVNSSLDTWDQSAGGTLIERK 124
            EL+ +YLRDAVYFHHPRY+AHLNCPVV+PALL E ++S++NSSLDTWDQSAGGTLIE++
Sbjct: 79  RELDQLYLRDAVYFHHPRYVAHLNCPVVLPALLAEVLVSSLNSSLDTWDQSAGGTLIEQR 138

Query: 125 LIDWTCARIGLGPAADGVFTSGGTQSNLQALLLAREEA--------------KAEDFADL 170
           LIDWTCARIGLG  ADGVFTSGGTQSNL  LLLARE                   + A L
Sbjct: 139 LIDWTCARIGLGSQADGVFTSGGTQSNLMGLLLAREHVCNRLPDHGGNLRHGLPAEAAGL 198

Query: 171 RIFASEASHFSVRKSAKLLGLGPDAVVSIPVDRDKRMQTVALARELERCARDGLVPMAVV 230
           RIFAS ASHFSV+KSA LLGLG DAV SI  D  + M   ALA+ L  C R G +PMAVV
Sbjct: 199 RIFASRASHFSVQKSAALLGLGYDAVRSIETDSAQCMSVPALAQALADCQRLGELPMAVV 258

Query: 231 ATGGTTDFGSIDPLPEIAGLCEQYGVWMHVDAAYGCGLLASLKYRDRITGIERADSVTVD 290
           AT GTTDFGSIDPLPEIA LC+ YGVW+HVDAAYG GLL + +YRD + GIE ADSVTVD
Sbjct: 259 ATAGTTDFGSIDPLPEIAALCQHYGVWLHVDAAYGGGLLVAPRYRDWLAGIEHADSVTVD 318

Query: 291 YHKSFFQPVSSSAVLVRDAATLRHATYHAEYLNPRRMVQERIPNQVDKSLQTTRRFDALK 350
           YHKSFFQPVS S   VR    L + T+HA+YLNPR   +E  PN V+KS+QTTRRFDALK
Sbjct: 319 YHKSFFQPVSCSGFFVRQRQHLSYITHHADYLNPRSQTREGTPNLVNKSIQTTRRFDALK 378

Query: 351 LWMTLRVMGADGIGVLFDEVCDLAAEGWKLLAADPRFDVVVQPSLSTLVFRHIPADVTDP 410
           LW+TLR++GA+ IG +F+EV D AA+  +LLA DP F+V   P LSTLVFR +   V + 
Sbjct: 379 LWLTLRILGAEHIGAMFEEVIDRAADTHRLLANDPAFEVFT-PRLSTLVFRFVAPGVAN- 436

Query: 411 AEIDRANLYARKALFASGDAVVAGTKVAGRHYLKFTLLNPETTPADIAAVLDLIAGHAEQ 470
             +D  N   RKA+F SG AV+A T V GR YLKFTLLNPETT  D+ A+++LI  H  +
Sbjct: 437 ERLDEINREIRKAIFRSGAAVIAATVVEGRQYLKFTLLNPETTLNDLRAIVELIRDHGAR 496

Query: 471 YLGD 474
            L +
Sbjct: 497 LLDE 500


Lambda     K      H
   0.320    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 667
Number of extensions: 18
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 480
Length of database: 507
Length adjustment: 34
Effective length of query: 446
Effective length of database: 473
Effective search space:   210958
Effective search space used:   210958
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources