GapMind for catabolism of small carbon sources

 

Aligments for a candidate for malF_Aa in Pseudomonas stutzeri RCH2

Align Binding-protein-dependent transport systems inner membrane component (characterized, see rationale)
to candidate GFF850 Psest_0864 ABC-type sugar transport systems, permease components

Query= uniprot:C8WUR0
         (321 letters)



>lcl|FitnessBrowser__psRCH2:GFF850 Psest_0864 ABC-type sugar
           transport systems, permease components
          Length = 521

 Score =  179 bits (455), Expect = 1e-49
 Identities = 98/252 (38%), Positives = 148/252 (58%), Gaps = 7/252 (2%)

Query: 64  FVGLKNYEELLNPHDPLSNLFLPTFIWTLVYALCTTALAYLVGLFLAVLLNNKHMRERTL 123
           F G  N+  +L     +   F+  F WT  +A  T      VGL LA LL  + +R +  
Sbjct: 267 FTGFANFSRVLT-EPSIREPFMQIFAWTFAFAGLTVVFTLAVGLVLASLLQWELVRGKAF 325

Query: 124 YRTLLIVPWAVPNLISMLAWQGLLNDQYGQINALLHGVFGLPRIPWLTSALWARIAVIMV 183
           YR +LI+P+AVP  IS+L ++GL N  +G+IN LL G+FG+ R  W +    AR  +++V
Sbjct: 326 YRLMLILPYAVPGFISILVFRGLFNQNFGEINLLLEGLFGI-RPDWFSDPSLARTMILIV 384

Query: 184 NVWAGFPYMMTVCLGALQSIPTDQYEAAEIDGANWWQVFRYVTMPSVWRISLPLLIPSFS 243
           N W G+PYM+ +C+G LQ+IP DQYEA+ IDGA+       +T+P + +  +PLLI  F+
Sbjct: 385 NTWLGYPYMLLLCMGLLQAIPRDQYEASAIDGASPLDNLLRITLPQLIKPLMPLLIACFA 444

Query: 244 YNFNNFNASYLLTGGGPP--NSNNPFLGQTDILATAAYKMTLTFNRYD--LGATISVLLF 299
           +NFNNF    LLT GGP    +  P  G TD+L +  Y++    +  D  L A I+ ++F
Sbjct: 445 FNFNNFVLITLLTRGGPDIIGATTP-AGTTDLLVSYTYRIAFQDSGQDFALAAAIATMIF 503

Query: 300 ILVALISWVQMR 311
           ILV  ++ + ++
Sbjct: 504 ILVGAMALLNLK 515



 Score = 32.7 bits (73), Expect = 2e-05
 Identities = 14/38 (36%), Positives = 23/38 (60%)

Query: 24  AYGYLSPALVTICVLSILPIFYTIYISFTNFNQMHFLS 61
           A  Y+ P++  + V  I P+ YT+ I FTN++  + LS
Sbjct: 77  AQRYIFPSVAGMLVFVIFPLLYTVGIGFTNYSGTNLLS 114


Lambda     K      H
   0.327    0.140    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 446
Number of extensions: 23
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 321
Length of database: 521
Length adjustment: 31
Effective length of query: 290
Effective length of database: 490
Effective search space:   142100
Effective search space used:   142100
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory