Align Maltose-transporting ATPase (EC 3.6.3.19) (characterized)
to candidate GFF1860 Psest_1899 ABC-type sugar transport systems, ATPase components
Query= reanno::psRCH2:GFF857 (371 letters) >lcl|FitnessBrowser__psRCH2:GFF1860 Psest_1899 ABC-type sugar transport systems, ATPase components Length = 390 Score = 306 bits (783), Expect = 8e-88 Identities = 167/343 (48%), Positives = 220/343 (64%), Gaps = 6/343 (1%) Query: 1 MASVTLRDICKSYDGTPIT--RHIDLDIEDGEFVVFVGPSGCGKSTLLRLIAGLEDITSG 58 MAS+ LR++ KSY + I + I L I+ GEF++ VGPSGCGKSTL+ IAGLE+IT G Sbjct: 1 MASLELRNVQKSYGNSQIATLKDIALKIDAGEFLILVGPSGCGKSTLMNCIAGLENITGG 60 Query: 59 DLLIDNQRVNDLPPKDRSVGMVFQSYALYPHMTVAENMAFGLKLASVDKREIKRRVEAVA 118 ++L+D + ++ PKDR + MVFQSYALYP M+V +N+AFGLK+ V +I+ V VA Sbjct: 61 EILVDGEDISQASPKDRDIAMVFQSYALYPTMSVRDNIAFGLKMRKVPAAKIEEEVARVA 120 Query: 119 EILQLDKLLERKPKDLSGGQRQRVAIGRTMVREPKVFLFDEPLSNLDAFLRVQMRIEIAR 178 ++LQ++ LLERKP LSGGQ+QRVA+GR + R PK++LFDEPLSNLDA LRV+MR EI Sbjct: 121 KLLQIEPLLERKPSQLSGGQQQRVAMGRALARRPKIYLFDEPLSNLDAKLRVEMRTEIKL 180 Query: 179 LHQRIRSTMIYVTHDQVEAMTLADKIVVLNAGEIAQVGQPLHLYHYPKNRFVAGFLGSPQ 238 +HQR+++T +YVTHDQ+EAMTL DK+ V+ G I Q G P +Y+ P N FVA F+GSP Sbjct: 181 MHQRLKTTTVYVTHDQIEAMTLGDKVAVMKDGVIQQFGTPHEIYNNPANLFVASFIGSPP 240 Query: 239 MNFVEVRAISASPETVTI--ELPSGYPLTLPVDGSAVSPGDPLTLGIRPEHFVM--PDEA 294 MNFV +R V + L LP+ L LGIRPE + A Sbjct: 241 MNFVPLRIRQRDGRWVGVLNSEQGSCELPLPITSDDGLRDRELILGIRPEQIGLAPAGSA 300 Query: 295 DFTFHGQITVAERLGQYNLLYLTLERLQDVITLCVDGNLRVTE 337 DF+ I V E G L+ TL +++ L D RV E Sbjct: 301 DFSLAVDIEVVEPTGPDTLVVFTLNQVKACCRLAPDQAPRVGE 343 Lambda K H 0.322 0.139 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 395 Number of extensions: 13 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 371 Length of database: 390 Length adjustment: 30 Effective length of query: 341 Effective length of database: 360 Effective search space: 122760 Effective search space used: 122760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory