Alignments for a candidate for paaK in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaK in Pseudomonas stutzeri RCH2

Align phenylacetate-CoA ligase (EC 6.2.1.30) (characterized)
to candidate GFF1301 Psest_1334 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Query= BRENDA::D3GE78
         (556 letters)



>FitnessBrowser__psRCH2:GFF1301
          Length = 562

 Score =  185 bits (469), Expect = 5e-51
 Identities = 153/522 (29%), Positives = 234/522 (44%), Gaps = 30/522 (5%)

Query: 46  LTTHDLRLWSQRLAAGLRK-SGLQRGDRVLLFSGNDLFFPVVFLGVIMAGGIFTGANPTF 104
           L+  DL   S    A L+  + LQ GDR+ +   N L +P+   G + AG I    NP +
Sbjct: 50  LSYSDLERHSAAFTAWLQHHTDLQPGDRIAIQMPNLLQYPIAVFGALRAGLIVVNTNPLY 109

Query: 105 VARELAYQLQDSGA---TYLLCASNSLE-----TGLEAAKQAKLPQSHIFAYDTSIYDGV 156
            +RE+ +Q QDSGA    YL    ++++     T +E   + ++           +   V
Sbjct: 110 TSREMRHQFQDSGARALVYLNTFGHNVQEVLPDTAIEHLIEVRIGDMLPPLKGVLVNAAV 169

Query: 157 TNPQKGCAYWS--------DLLASEEEGAAFTWDELSTPALSSTTLALNYSSGTTGRPKG 208
            + +K    +S        D+L    +GA    +    P        L Y+ GTTG  KG
Sbjct: 170 KHLKKMVPDYSLPQAISFKDVL---RDGARH--NHKPAPLELDHVAVLQYTGGTTGVAKG 224

Query: 209 VEISHRNYVANMLQY---CHTASLHPDYKARLERSRWLCFLPMYHAMAQNIFIAAALYRA 265
             ++H N VANM Q          H     R  +   +  LP+YH  A  +     +   
Sbjct: 225 AMLTHGNLVANMQQVRANMQQLDEHGHPIIREGQEVMIAPLPLYHIYAFTVNCMCMVVTG 284

Query: 266 TP-VYIMSKFDFVKMLEYTQRFRITDFILVPPVVVALAKHPAVGQYDLSSVELVGSGAAP 324
              V I +  D    ++  QR++ + F+ +  + VAL  HP   + D S ++   SG   
Sbjct: 285 NHNVLITNPRDINGFVKELQRWQFSAFLGLNTLFVALMAHPQFKKIDFSRLKGTNSGGTA 344

Query: 325 LGREVCEEVEKLWPPGKINIKQGWGMTEATCSVTGWNPAEISTSASVGELNANCEAKIMF 384
           L   V E  + +       + +G+G+TE +  V      E S   +VG        K++ 
Sbjct: 345 LVSAVAERWKSMTG---CTVVEGYGLTETSPVVCANPHGEHSRLGTVGLPVPGTTVKVID 401

Query: 385 DGVEVKERNSRGELWVRAPNVMKGYWRNEKATKETKTEDGWLLTGDIAFVDDDGKFHVVD 444
           D         RGEL V+ P VMKGYW+   AT E   E+GWL TGDIA +D+DG   +VD
Sbjct: 402 DEGNALPLGERGELCVKGPQVMKGYWQRPDATAEVLDEEGWLKTGDIAVIDEDGFVSIVD 461

Query: 445 RMKELIKVKGNQVAPAELEALLLEHPAISDVAVIGVVINNDERPRAYVVLRPGQSATANE 504
           R K+LI V G  V P E+E +++ HP ++  A IGV            V+    +   +E
Sbjct: 462 RKKDLIIVSGFNVYPNEIEDVVMAHPKVAACAAIGVADEKSGEAVKLFVVPSDPTLDQDE 521

Query: 505 IAHYLDNKVSAFKRITGGVVFLEAIPKNPSGKILRMKLREQA 546
           +  Y     + +K +    VF +A+P  P GKILR +LR+ A
Sbjct: 522 LHAYCRENFTGYK-MPRHYVFRDALPMTPVGKILRRELRDIA 562


Lambda     K      H
   0.319    0.134    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 725
Number of extensions: 37
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 556
Length of database: 562
Length adjustment: 36
Effective length of query: 520
Effective length of database: 526
Effective search space:   273520
Effective search space used:   273520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources