Alignments for a candidate for pad-dh in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for pad-dh in Pseudomonas stutzeri RCH2

Align aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)
to candidate GFF882 Psest_0905 NAD-dependent aldehyde dehydrogenases

Query= BRENDA::P05091
         (517 letters)



>FitnessBrowser__psRCH2:GFF882
          Length = 499

 Score =  395 bits (1015), Expect = e-114
 Identities = 211/489 (43%), Positives = 303/489 (61%), Gaps = 10/489 (2%)

Query: 28  PNQQPEVFCNQIFINNEWHDAVSRKTFPTVNPSTGEVICQVAEGDKEDVDKAVKAARAAF 87
           PN  P+     +FI+N+W      +T   +NP+ G+++  +      DVD+AV+AA+ AF
Sbjct: 9   PNHLPDSSYG-LFIDNQWVSDEYGETLDIINPANGKILTNIPNATAADVDRAVQAAQRAF 67

Query: 88  QLGSPWRRMDASHRGRLLNRLADLIERDRTYLAALETLDNGKPYVISYLVDLDMVLKCLR 147
                WR    + R   L ++ADL+E D    A LETLD GKP   S  VD+ + +   R
Sbjct: 68  MT---WRTTSPAERANALLKIADLLEADADRFAVLETLDVGKPIRESRSVDIPLAIDHFR 124

Query: 148 YYAGWADKYHGKTIPIDGDFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVV 207
           Y+AG       + + +D    S    EP+GV GQ+IPWNFPLLM AWK+ PA+A GN VV
Sbjct: 125 YFAGVIRSQSDEAVMLDEQTLSIALSEPLGVVGQVIPWNFPLLMAAWKIAPAIAAGNTVV 184

Query: 208 MKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIG 267
           +K +E TP+T L +A +  +   P GVVNIV G G T G A+  H D+ K+AFTGST +G
Sbjct: 185 IKPSELTPVTILELAKIFAKV-LPAGVVNIVTGLGTTVGQALLDHPDLRKLAFTGSTRVG 243

Query: 268 RVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFV 327
            ++  AA +  +   TLELGGKS NI+  DA+ D AVE A  A+ +NQGQ C +G+R FV
Sbjct: 244 ELVANAA-AKKIIPATLELGGKSANIVFPDANWDKAVEGAVLAILWNQGQVCESGARLFV 302

Query: 328 QEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCG 387
            E IY+ F+     + ++  VG+P +  T  G QV ++Q ++ILGY++  KQEGA++L G
Sbjct: 303 HESIYERFLAELKHKFEAVRVGDPLNPDTMMGAQVSKSQMERILGYVDIAKQEGAEVLIG 362

Query: 388 GGIAA----DRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYG 443
           GG       D G+FIQPT+   V++ M +A EEIFGPV+ ++ FK   EV+  AN+S YG
Sbjct: 363 GGRLTGANYDAGFFIQPTILVGVRNDMRVAYEEIFGPVLCVIPFKDEAEVIAMANDSEYG 422

Query: 444 LAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYT 503
           LA AV+T+D+++A  +++A++ G +WVN Y    A +PFGGYK SG GRE  +  L+AY+
Sbjct: 423 LAGAVWTQDINRALRVARAVETGRMWVNTYHEIPAHAPFGGYKKSGLGRETHKSMLEAYS 482

Query: 504 EVKTVTVKV 512
           + K + V +
Sbjct: 483 QKKNIYVSL 491


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 580
Number of extensions: 27
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 517
Length of database: 499
Length adjustment: 34
Effective length of query: 483
Effective length of database: 465
Effective search space:   224595
Effective search space used:   224595
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources