Align Glycine betaine/proline betaine transporter BetS (characterized)
to candidate GFF2585 Psest_2635 choline/carnitine/betaine transport
Query= SwissProt::G3XCN6 (706 letters) >FitnessBrowser__psRCH2:GFF2585 Length = 688 Score = 511 bits (1316), Expect = e-149 Identities = 262/654 (40%), Positives = 392/654 (59%), Gaps = 18/654 (2%) Query: 52 KVNLPVFVGSVAVIALFVGIGVIAPKRAESIFSGMQTAILSGFGWLYLLSVAVFLFSMLF 111 ++N VF GS I LF + R+ ++ + + I + FGW Y L+V ++L ++ Sbjct: 17 RINPVVFYGSAIAIVLFAVWTMFFTDRSLAVINTVLGWISNTFGWFYFLAVLLYLLFVVV 76 Query: 112 LAFSRYGELKLGPDDSEPEFRYLSWIAMLFAAGMGIGLMYFAVGEPMTHFASPPEAEPLT 171 +A SRYG+++LGPD S+PEF ++W AMLF+AG+GI L++F + EP+T F PP E + Sbjct: 77 VATSRYGKIRLGPDHSKPEFNVVTWAAMLFSAGIGIDLLFFCIAEPITQFLEPPVGEGGS 136 Query: 172 IAAQREAMSVTFFHWGVHAWAIYSVVGLSLAYFGYRYNLPLTVRSGLYPLLKEGIHGPIG 231 A R AM +TF HWG+ W +Y++VG+SLAYF +R LPL++RS LYP+ + I+GPIG Sbjct: 137 TQAARHAMELTFLHWGLSGWGVYTLVGMSLAYFSFRQGLPLSIRSSLYPIFGKRIYGPIG 196 Query: 232 HVVDIFAICGTMFGLATSLGFGILQINSGLNYLLGIPQSIYVQLLLVTVVTAIATISVVT 291 H VD A+ GT+FG+ATSLG GI+Q+N GLNY+ GIP+ Q +LV ++ + IS T Sbjct: 197 HTVDTAAVLGTIFGIATSLGIGIIQLNFGLNYIFGIPEGTLTQAVLVVLIVVFSAISAAT 256 Query: 292 GVEKGVRILSETNLFLAVLLMLFVLVVGPTGTLMRDFVQNIGLYLDSLVLRTFNIYAY-E 350 GVE+G+R LSE N+ LAVLL+LFVLVVG T L+ V N+G Y + + + + YAY Sbjct: 257 GVERGIRRLSEFNMLLAVLLLLFVLVVGDTVFLLNALVMNVGDYFSNFISLSLDTYAYRR 316 Query: 351 PRPWIDSWTLFYWAWWISWSPFVGMFIARISRGRTVREFVTAVLFVPAMFTFLWMTVFGN 410 P W+++WT+F+WAWWI+W PFVG+F+ARISRGRT+REFV L +P F WM++ GN Sbjct: 317 PTDWLNAWTVFFWAWWIAWGPFVGLFLARISRGRTIREFVIGTLLLPLAFMMAWMSIMGN 376 Query: 411 TAIYVDTTIANG--ELARDVKADLSVALFQFFEYLPWPAVTSTLAVLLVSIFFVTSSDSG 468 +AI + + G E + ++ +++ F + LPW +T+ + +L +FFVTS DSG Sbjct: 377 SAIEM---VMGGALEFGQQAVSNPGSSIYLFLQSLPWAGLTTIVVTILAIVFFVTSGDSG 433 Query: 469 SLVID---TIASGGETATPALQRIFWCSLSGIVAAVLLSTGGLTALQSATISTALPFSLV 525 SLV+ +I + P RI W ++ G++ LL GGLTALQ + LPFS+V Sbjct: 434 SLVLSNFTSILKDVNSDAPVWMRILWAAIIGVLTLALLIAGGLTALQGTVVIMGLPFSIV 493 Query: 526 MLILVWSLF-----VGMRADLARTQSPGSLGPRA-YPASGVPWQRRLAMTLSTPDRRAVE 579 +L +++ LF G+ AD + G L RA + + W +RL+ +S P R + Sbjct: 494 LLFMMYGLFKALHVEGVMADSYQKSLSGYLSGRATLEHTQMNWSQRLSRAMSFPSRSQIR 553 Query: 580 KFLQASVLPALEAVARELTRRSRPASVGRDAETG--ALTLTVPAEGHRDFVYGVQMSEHK 637 ++L+ PA+E + + L + P + + E G L L V +DF Y + Sbjct: 554 RYLKDVCKPAMEEIQKALGEKGVPVDI-VEGEPGNEHLALHVNLGSEQDFTYQIWPVRGT 612 Query: 638 LPAFTAYDATVADVRYEARTFFSDGSRGYDIMGMADNQIINDVLFQFERYTGFV 691 +P+F + Y GS GYD+MG + Q+I D+L +E + F+ Sbjct: 613 MPSFALRTQSGNADYYRLEVHLRQGSLGYDLMGYSRRQLIEDILDHYEHHMHFL 666 Lambda K H 0.326 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1290 Number of extensions: 66 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 706 Length of database: 688 Length adjustment: 39 Effective length of query: 667 Effective length of database: 649 Effective search space: 432883 Effective search space used: 432883 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory