Align Aconitate hydratase A; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 (characterized)
to candidate GFF2274 Psest_2319 aconitate hydratase 1/2-methylisocitrate dehydratase, Fe/S-dependent
Query= SwissProt::Q937N8 (869 letters) >FitnessBrowser__psRCH2:GFF2274 Length = 867 Score = 1433 bits (3710), Expect = 0.0 Identities = 712/866 (82%), Positives = 781/866 (90%), Gaps = 6/866 (0%) Query: 1 MNSANRKPLPGTKLDYFDARAAVEAIQPGAYDKLPYTSRVLAENLVRRCDPATLTDSLLQ 60 MN+ +RKPLPGT LDYFD R A+EAIQPG+YDKLPYTSRVLAE LVRRC+P LTDSL Q Sbjct: 1 MNTEHRKPLPGTGLDYFDTREAIEAIQPGSYDKLPYTSRVLAEQLVRRCEPEALTDSLKQ 60 Query: 61 LVGRKRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIADQGGDPAKVNPVVPVQLIVDH 120 ++ RKRDLDFPW+PARVVCHDILGQTALVDLAGLRDAIA+QGGDPAKVNPVVP QLIVDH Sbjct: 61 IIERKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAIAEQGGDPAKVNPVVPTQLIVDH 120 Query: 121 SLAVECGGFDPDAFAKNRAIEDRRNEDRFHFIDWTKQAFKNVDVIPPGNGIMHQINLEKM 180 SLAVE GFDPDAF KNRA+E+RRNEDRFHFI+WTK AFKNVDVIP GNGIMHQINLEKM Sbjct: 121 SLAVEFAGFDPDAFEKNRAVEERRNEDRFHFIEWTKTAFKNVDVIPAGNGIMHQINLEKM 180 Query: 181 SPVIHADNGVAYPDTCVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIVGV 240 SPVI A GVA+PDTCVGTDSHTPHVDALGVIAIGVGGLEAE VMLG S MRLPDIVGV Sbjct: 181 SPVIQARGGVAFPDTCVGTDSHTPHVDALGVIAIGVGGLEAETVMLGLPSMMRLPDIVGV 240 Query: 241 ELTGKRQPGITATDIVLALTEFLRKEKVVGAYLEFRGEGASSLTLGDRATISNMAPEYGA 300 LTGKRQPGITATDIVLALTEFLRKE+VVGA++EF GEGA SLT+GDRATISNM PEYGA Sbjct: 241 RLTGKRQPGITATDIVLALTEFLRKERVVGAWVEFFGEGADSLTIGDRATISNMCPEYGA 300 Query: 301 TAAMFFIDEQTIDYLRLTGRTDEQLKLVETYARTAGLWADSLKNAEYERVLKFDLSSVVR 360 TA+MF+ID+QTIDYL+LTGR EQ+ LVE YA+ GLWA +L+ AEYERVL+FDLSSVVR Sbjct: 301 TASMFYIDQQTIDYLKLTGREPEQVALVEQYAKETGLWATALEGAEYERVLEFDLSSVVR 360 Query: 361 NMAGPSNPHKRLPTSALAERGIAVDLDK---ASAQEAEGLMPDGAVIIAAITSCTNTSNP 417 NMAGPSNPHKRLPTSAL ERGIA D DK A A+EAEGL+PDGAVIIAAITSCTNTSNP Sbjct: 361 NMAGPSNPHKRLPTSALHERGIA-DEDKLAAARAEEAEGLLPDGAVIIAAITSCTNTSNP 419 Query: 418 RNVIAAALLARNANARGLARKPWVKSSLAPGSKAVELYLEEANLLPDLEKLGFGIVAFAC 477 RNV+AA LLA+ AN GL RKPWVK+S APGSK +LYLEEA LL +LEKLGFGIVA+AC Sbjct: 420 RNVVAAGLLAKKANELGLVRKPWVKTSFAPGSKVAKLYLEEAGLLSELEKLGFGIVAYAC 479 Query: 478 TTCNGMSGALDPKIQQEIIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIA 537 TTCNGMSGALDP IQQEII+RDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIA Sbjct: 480 TTCNGMSGALDPVIQQEIIERDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIA 539 Query: 538 GTIRFDIEKDVLGTDQDGKPVYLKDIWPSDEEIDAIVAKSVKPEQFRKVYEPMFAITAAS 597 GT+RFDIE+DVLGTD++G P+ LKD+WPSDEEIDAIVA SVKPEQF+++Y PMF + Sbjct: 540 GTVRFDIEQDVLGTDKNGNPITLKDLWPSDEEIDAIVASSVKPEQFKQIYIPMFDLGTIE 599 Query: 598 GESVSPLYDWRPQSTYIRRPPYWEGALAGERTLKALRPLAVLGDNITTDHLSPSNAIMLN 657 E+ SPLYDWRP STYIRRPPYWEGALAGERTLK +RPLA+L DNITTDHLSPSNAI+L+ Sbjct: 600 -EAKSPLYDWRPMSTYIRRPPYWEGALAGERTLKGMRPLAILPDNITTDHLSPSNAILLD 658 Query: 658 SAAGEYLARMGLPEEDFNSYATHRGDHLTAQRATFANPTLINEMAVVDGQVKKGSLARIE 717 SAAGEYLA+MGLPEEDFNSYATHRGDHLTAQRATFANP L+NEMAVVDG+ +KGSLAR+E Sbjct: 659 SAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRATFANPQLVNEMAVVDGKAQKGSLARVE 718 Query: 718 PEGKVVRMWEAIETYMDRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEVIVAEGFERIH 777 PEGKV+RMWEAIETYM+RKQ LII+AGADYGQGSSRDWAAKGVRLAGVEVIVAEGFERIH Sbjct: 719 PEGKVMRMWEAIETYMNRKQNLIIVAGADYGQGSSRDWAAKGVRLAGVEVIVAEGFERIH 778 Query: 778 RTNLIGMGVLPLEFKPGVNRLTLGLDGTETYDVIGERQPRATLTLVVNRKNGERVEVPVT 837 RTNL+GMGVLP+EFKPG RLTLGLDGTET+D+ GE PR LTLV++ +GE VPVT Sbjct: 779 RTNLVGMGVLPVEFKPGTTRLTLGLDGTETFDIEGELSPRCDLTLVIHHMSGEETRVPVT 838 Query: 838 CRLDSDEEVSIYEAGGVL-HFAQDFL 862 CRLD+ EVS+Y+AGGVL FA+DFL Sbjct: 839 CRLDTAAEVSVYQAGGVLQRFAKDFL 864 Lambda K H 0.318 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2244 Number of extensions: 74 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 869 Length of database: 867 Length adjustment: 42 Effective length of query: 827 Effective length of database: 825 Effective search space: 682275 Effective search space used: 682275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
Align candidate GFF2274 Psest_2319 (aconitate hydratase 1/2-methylisocitrate dehydratase, Fe/S-dependent)
to HMM TIGR02333 (acnD: 2-methylisocitrate dehydratase, Fe/S-dependent (EC 4.2.1.99))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02333.hmm # target sequence database: /tmp/gapView.10034.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02333 [M=858] Accession: TIGR02333 Description: 2met_isocit_dHY: 2-methylisocitrate dehydratase, Fe/S-dependent Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 0 1775.3 0.0 0 1775.1 0.0 1.0 1 lcl|FitnessBrowser__psRCH2:GFF2274 Psest_2319 aconitate hydratase 1 Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__psRCH2:GFF2274 Psest_2319 aconitate hydratase 1/2-methylisocitrate dehydratase, Fe/S-dependent # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 1775.1 0.0 0 0 1 857 [. 2 864 .. 2 865 .. 0.99 Alignments for each domain: == domain 1 score: 1775.1 bits; conditional E-value: 0 TIGR02333 1 ntkyrkalpgtdldyfdaraaveaikpgaydklpytsrvlaenlvrrvdpetleaslkqlierkreldfpwypar 75 nt++rk+lpgt ldyfd+r+a+eai+pg+ydklpytsrvlae+lvrr++pe l++slkq+ierkr+ldfpwypar lcl|FitnessBrowser__psRCH2:GFF2274 2 NTEHRKPLPGTGLDYFDTREAIEAIQPGSYDKLPYTSRVLAEQLVRRCEPEALTDSLKQIIERKRDLDFPWYPAR 76 7999*********************************************************************** PP TIGR02333 76 vvchdilgqtalvdlaglrdaiaekggdpaqvnpvvetqlivdhslaveyggfdpdafeknraiedrrnedrfhf 150 vvchdilgqtalvdlaglrdaiae+ggdpa+vnpvv+tqlivdhslave++gfdpdafeknra+e+rrnedrfhf lcl|FitnessBrowser__psRCH2:GFF2274 77 VVCHDILGQTALVDLAGLRDAIAEQGGDPAKVNPVVPTQLIVDHSLAVEFAGFDPDAFEKNRAVEERRNEDRFHF 151 *************************************************************************** PP TIGR02333 151 inwtkkafknvdvipagngimhqinlekmspvvqvkegvafpdtlvgtdshtphvdalgviaigvggleaetvml 225 i+wtk+afknvdvipagngimhqinlekmspv+q++ gvafpdt+vgtdshtphvdalgviaigvggleaetvml lcl|FitnessBrowser__psRCH2:GFF2274 152 IEWTKTAFKNVDVIPAGNGIMHQINLEKMSPVIQARGGVAFPDTCVGTDSHTPHVDALGVIAIGVGGLEAETVML 226 *************************************************************************** PP TIGR02333 226 graslmrlpdivgveltgkrqpgitatdivlalteflrkekvvsayleffgegakaltlgdratisnmtpeygat 300 g +s+mrlpdivgv+ltgkrqpgitatdivlalteflrke+vv+a++effgega +lt+gdratisnm+peygat lcl|FitnessBrowser__psRCH2:GFF2274 227 GLPSMMRLPDIVGVRLTGKRQPGITATDIVLALTEFLRKERVVGAWVEFFGEGADSLTIGDRATISNMCPEYGAT 301 *************************************************************************** PP TIGR02333 301 aamfaideqtidylkltgreeeqvklvetyakaaglwadslkkavyervlkfdlssvvrnlagpsnpharlatsd 375 a+mf+id+qtidylkltgre+eqv+lve yak +glwa +l+ a+yervl+fdlssvvrn+agpsnph+rl+ts lcl|FitnessBrowser__psRCH2:GFF2274 302 ASMFYIDQQTIDYLKLTGREPEQVALVEQYAKETGLWATALEGAEYERVLEFDLSSVVRNMAGPSNPHKRLPTSA 376 *************************************************************************** PP TIGR02333 376 laakgiakevee......eaeglmpdgaviiaaitsctntsnprnvvaagllarnanklglkrkpwvksslapgs 444 l +gia e + eaegl+pdgaviiaaitsctntsnprnvvaaglla++an+lgl rkpwvk+s+apgs lcl|FitnessBrowser__psRCH2:GFF2274 377 LHERGIADEDKLaaaraeEAEGLLPDGAVIIAAITSCTNTSNPRNVVAAGLLAKKANELGLVRKPWVKTSFAPGS 451 ******98754333444499******************************************************* PP TIGR02333 445 kvvklyleeagllkeleklgfgivafacttcngmsgaldpviqqeiidrdlyatavlsgnrnfdgrihpyakqaf 519 kv+klyleeagll+eleklgfgiva+acttcngmsgaldpviqqeii+rdlyatavlsgnrnfdgrihpyakqaf lcl|FitnessBrowser__psRCH2:GFF2274 452 KVAKLYLEEAGLLSELEKLGFGIVAYACTTCNGMSGALDPVIQQEIIERDLYATAVLSGNRNFDGRIHPYAKQAF 526 *************************************************************************** PP TIGR02333 520 laspplvvayaiagtirfdiekdvlgvdadgkeirlkdiwpsdeeidavvaaavkpeqfrkvyipmfdledaqkk 594 laspplvvayaiagt+rfdie+dvlg+d++g++i lkd+wpsdeeida+va++vkpeqf+++yipmfdl++ +++ lcl|FitnessBrowser__psRCH2:GFF2274 527 LASPPLVVAYAIAGTVRFDIEQDVLGTDKNGNPITLKDLWPSDEEIDAIVASSVKPEQFKQIYIPMFDLGTIEEA 601 *************************************************************************** PP TIGR02333 595 vsplydwrpmstyirrppywegalagertlkgmrplavlgdnittdhlspsnailldsaageylakmglpeedfn 669 splydwrpmstyirrppywegalagertlkgmrpla+l+dnittdhlspsnailldsaageylakmglpeedfn lcl|FitnessBrowser__psRCH2:GFF2274 602 KSPLYDWRPMSTYIRRPPYWEGALAGERTLKGMRPLAILPDNITTDHLSPSNAILLDSAAGEYLAKMGLPEEDFN 676 *************************************************************************** PP TIGR02333 670 syathrgdhltaqratfanpklfnemvkedgkvkqgslariepegkvtrmweaietymnrkqpliiiagadygqg 744 syathrgdhltaqratfanp+l+nem+ +dgk ++gslar+epegkv+rmweaietymnrkq lii+agadygqg lcl|FitnessBrowser__psRCH2:GFF2274 677 SYATHRGDHLTAQRATFANPQLVNEMAVVDGKAQKGSLARVEPEGKVMRMWEAIETYMNRKQNLIIVAGADYGQG 751 *************************************************************************** PP TIGR02333 745 ssrdwaakgvrlagveaivaegferihrtnlvgmgvlplefkpgtnrktlaldgtevydvvgeitpradltlvvt 819 ssrdwaakgvrlagve ivaegferihrtnlvgmgvlp+efkpgt r tl+ldgte++d+ ge +pr+dltlv++ lcl|FitnessBrowser__psRCH2:GFF2274 752 SSRDWAAKGVRLAGVEVIVAEGFERIHRTNLVGMGVLPVEFKPGTTRLTLGLDGTETFDIEGELSPRCDLTLVIH 826 *************************************************************************** PP TIGR02333 820 rkngeklevpvtcrldtaeevsvyeaggvlqrfaqdfl 857 ge+ vpvtcrldta evsvy+aggvlqrfa+dfl lcl|FitnessBrowser__psRCH2:GFF2274 827 HMSGEETRVPVTCRLDTAAEVSVYQAGGVLQRFAKDFL 864 *************************************8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (858 nodes) Target sequences: 1 (867 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.07u 0.03s 00:00:00.10 Elapsed: 00:00:00.09 # Mc/sec: 8.22 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory