Align L-lactate permease (characterized, see rationale)
to candidate GFF3767 Psest_3836 L-lactate permease
Query= uniprot:A0KZB2 (547 letters) >FitnessBrowser__psRCH2:GFF3767 Length = 567 Score = 462 bits (1189), Expect = e-134 Identities = 248/553 (44%), Positives = 352/553 (63%), Gaps = 16/553 (2%) Query: 2 TILQLLASLTPVISVMIFLVLLRMPASKAMPISMIITALAAVFIWQMDTTLLAASVLEGL 61 T+L +LA P++ + L+ R PA AMP+ ++TAL + W M + AS L+GL Sbjct: 4 TLLSILA-FVPLVLAGVLLIGFRWPAKYAMPLVFVLTALIGLLAWDMTLNRVIASTLQGL 62 Query: 62 LSAITPLTIIFGAVFLLNTLKYSGAMDTIRAGFTNISADARVQVIIICWLFGAFIEGSAG 121 + L IIFGA+ LLNTLK+SG + +IR GF+N+S D RVQV+I+ WLFG FIEG++G Sbjct: 63 ILTAAILWIIFGAILLLNTLKHSGGISSIRRGFSNVSPDRRVQVLIVAWLFGCFIEGASG 122 Query: 122 FGTPAAIGAPLLVLLGVPPVAAAVVALIADSACVSFGAIGLPVLFGMEQGLTQGGVSLAA 181 FGTPAA+ APL+V LG P +AA V+ ++ S VSFGA+G P+L G+ GL + G+S Sbjct: 123 FGTPAAVAAPLMVALGFPALAAVVMGMMVQSTPVSFGAVGTPILVGVGAGLDKTGIS--- 179 Query: 182 EQFAAHGGSYVGYARFIVMHMITIDLITGTLIPLVMVTILTGFFGRNKSFKEGLAIWKFA 241 EQ AA G ++ + I + I + G L+PL+MV+I+T +FGRNKS+ EGLA+ FA Sbjct: 180 EQLAAVGSNWEVFFHLIFSRVAIIHALCGILMPLIMVSIMTRYFGRNKSWTEGLAVAPFA 239 Query: 242 IFAGLAFTVPAWIINYLAGPEFPSVIGSLVGMALVIPVARKGYLLPKTPWNDFA---END 298 +F GLAF +P GPEFPS+IG+LVG+A+V+P A+ G+LLPK W DFA E Sbjct: 240 VFTGLAFVIPYAAAGVFLGPEFPSMIGALVGLAIVVPAAKAGFLLPKDTW-DFAPAKEWP 298 Query: 299 SQEGAKIETTAK-------FSQIAAWTPYIIMAALLVLSRTVAPLKAWLSSFNISWTGLM 351 S+ KIE + S AW PY+++A LLV SR KA L S + W ++ Sbjct: 299 SEWMGKIEMKIEDVAGKTPISVPMAWAPYLLLAGLLVASRVFPDFKALLMSLSFGWKDIL 358 Query: 352 G-TELKASFATLYAPGAFFVAVCILGFFLFKMKSPAIKQSIGVSCKSMLPTIISLGASVP 410 G T + + LY PG V ++ FFL +MK+ + +I S K++L L ++P Sbjct: 359 GETGVSGTLEPLYLPGGILCMVVLITFFLHRMKASELGAAISESSKTLLGAGFVLIFTIP 418 Query: 411 MVKIFLNSGVNGAGLASMPVALADMLGQSMGAVWAWMAPIVGIFGAFLSGSATFSNMMFS 470 MV+I +NSGVNG+ L SMPVA+A ++ S+G+V+ + AP VG GAF++GS T SN+M S Sbjct: 419 MVRILINSGVNGSDLVSMPVAMAQLVANSVGSVYPFFAPAVGALGAFIAGSNTVSNLMLS 478 Query: 471 SLQYSVADNIGMNHTLVLALQGIGANAGNMMCVMNVVAAATVVGMAGRESEIIRKTMPVA 530 Q++ A +G++ L++ALQ +GA AGNM+ + NVVAA+ VG+ GRE +RKTM Sbjct: 479 QFQFNTAGLLGLSGALMVALQSVGAAAGNMIAIHNVVAASATVGLLGREGITLRKTMLPT 538 Query: 531 IGYALLAGTIATL 543 + Y +LAG I + Sbjct: 539 LYYLILAGAIGLI 551 Lambda K H 0.326 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 965 Number of extensions: 57 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 547 Length of database: 567 Length adjustment: 36 Effective length of query: 511 Effective length of database: 531 Effective search space: 271341 Effective search space used: 271341 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory