Align Monocarboxylic acid transporter (characterized)
to candidate GFF2882 Psest_2938 transporter, SSS family
Query= SwissProt::Q8NS49 (551 letters) >FitnessBrowser__psRCH2:GFF2882 Length = 551 Score = 426 bits (1094), Expect = e-123 Identities = 248/557 (44%), Positives = 343/557 (61%), Gaps = 35/557 (6%) Query: 7 LAQDAVSEGVGNPILN---ISVFVVFIIVTMTVVLRVGKSTSESTDFYTGGASFSGTQNG 63 L DA++ V N I +FVVFI TM + K + + D+YT G S +G QNG Sbjct: 18 LLADAITGEVEKQATNYTAIIMFVVFIAFTMGITKWAAKRNTSTADYYTAGGSITGFQNG 77 Query: 64 LAIAGDYLSAASFLGIVGAISLNGYDGFLYSIGFFVAWLVALLLVAEPLRNVGRFTMADV 123 LAIAGD++SAASFLGI + +GYDG +YSIGF V W + L L+AE LRN+G+FT +DV Sbjct: 78 LAIAGDFMSAASFLGISALVYTSGYDGLIYSIGFLVGWPIILFLMAERLRNLGKFTFSDV 137 Query: 124 LSFRLRQKPVRVAAACGTLAVTLFYLIAQMAGAGSLVSVLLDIHEFKWQAVVVGIVGIVM 183 S+RL Q +R+ +A G+L V FYLIAQM GAG L+ +L + + V V +VG++M Sbjct: 138 ASYRLGQTQIRLLSAFGSLIVVAFYLIAQMVGAGKLIQLLFGLDYY----VAVVLVGVLM 193 Query: 184 IAYVLLGGMKGTTYVQMIKAVLLVGGVAIMTVLTFVKVSGGLTTLLNDAVEKHAASDYAA 243 + YVL GGM TT+VQ+IKAVLL+ G + M ++ V +L +AV+ H Sbjct: 194 VMYVLFGGMLATTWVQIIKAVLLLSGASFMAIMVMKSVGFDFGSLFAEAVKIH------- 246 Query: 244 TKGYDPTQILEPGLQYGATLTTQLDFISLALALCLGTAGLPHVLMRFYTVPTAKEARKSV 303 KG QI+ P G ++ + ISL LAL GTAGLPH+LMRF+TV AKEARKSV Sbjct: 247 EKG---AQIMSP----GGLVSDPISAISLGLALMFGTAGLPHILMRFFTVSDAKEARKSV 299 Query: 304 TWAIVLIGAFYLMTLVLGYGAAALVG-----PDRVIAAPGAANAAAPLLAFELGGSIFMA 358 +A IG FY++T ++G+GA LV D A G N A LA +GG++F+ Sbjct: 300 FYATGFIGYFYILTFIIGFGAILLVSTNPEFKDVTGAIVGGTNMVAIHLASAVGGNLFLG 359 Query: 359 LISAVAFATVLAVVAGLAITASAAVGHDIYNAVIRNGQSTEAEQVRVSRITVVVIGLISI 418 ISAVAFAT+LAVVAGL + ++AV HD+Y VI+ G++ E +++RV+++T + +G+++I Sbjct: 360 FISAVAFATILAVVAGLTLAGASAVSHDLYACVIKQGKAREEDEMRVTKLTTLTLGVVAI 419 Query: 419 VLGILAMTQNVAFLVALAFAVAASANLPTILYSLYWKKFNTTGAVAAIYTGLISALLLIF 478 +LGI+ QN+AF+V LAF++AAS N P + S+YWK +T GA+ GL +ALLL Sbjct: 420 LLGIIFEKQNIAFMVGLAFSIAASCNFPVLFLSMYWKGLSTRGALFGGSLGLFTALLLTI 479 Query: 479 LSPAVSGNDSAMVPGADWAIFPLKNPGLVSIPLAFIAGWIGTLVGKP----DNMDDLAAE 534 +SP V + V G AIFP K P L S+ AF W ++ K + + A+ Sbjct: 480 ISPTVWVD----VFGFAEAIFPYKYPALFSMAAAFAGIWFFSVTDKSKRAGEERERFFAQ 535 Query: 535 MEVRSLTGVGVEKAVDH 551 VRS TG+G AV H Sbjct: 536 F-VRSQTGLGATGAVAH 551 Lambda K H 0.324 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 712 Number of extensions: 41 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 551 Length of database: 551 Length adjustment: 36 Effective length of query: 515 Effective length of database: 515 Effective search space: 265225 Effective search space used: 265225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory