Alignments for a candidate for cstA in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for cstA in Pseudomonas stutzeri RCH2

Align Carbon starvation protein A (characterized, see rationale)
to candidate GFF3825 Psest_3894 Carbon starvation protein, predicted membrane protein

Query= uniprot:A0A0C4Y7X7
         (690 letters)



>FitnessBrowser__psRCH2:GFF3825
          Length = 558

 Score =  262 bits (670), Expect = 3e-74
 Identities = 186/571 (32%), Positives = 286/571 (50%), Gaps = 77/571 (13%)

Query: 32  VSALWIVVAAICIYLIAYRYYSKFIAEKVMQLDPKRMTPAWRHNDGLDYVPTNKAVLFGH 91
           +SA+ +++  +    + Y +YSKFIAE++ +LDP  +TPA    DG+DYVPTNK VL+GH
Sbjct: 1   MSAIALLLVGLIAMALGYVFYSKFIAERIYRLDPHYLTPAHTMRDGVDYVPTNKFVLWGH 60

Query: 92  HFAAIAGAGPLVGPVLAAQMGYMPGMLWILAGVVFAGAVQDFMVLFISTRRDGRSLGDLI 151
           HF ++AGA P+VGP +A   G+ P   W++ G +F   V DF  L+ S R  G+S+G L 
Sbjct: 61  HFTSVAGAAPIVGPAIAVIWGWGPAFAWVIFGTIFFAGVHDFGALWASARSRGQSVGMLS 120

Query: 152 KSEMGTVPGMIALFGCFMIMIIILAVLALIVVKALAGSPWGTFTVGVTIPIALFMG--IY 209
              +G     + L   F++++++    A ++   L  +P     V   I +AL +G  IY
Sbjct: 121 GRLIGARGRSLFLVVIFLVLLMVNGAFAAVISNLLVSTPTSVIPVWGAILVALVIGQMIY 180

Query: 210 ---TRYIRPGRIGEVSVIGFVLLMLAIIGGQYVHESAVLAPLFTYDGKALTWMLI--IYG 264
               + + P   G +     VL  L ++G QY    A+   +     K++ W+L+  +Y 
Sbjct: 181 RYNMKLLWPSLGGVI-----VLYALILLGNQY--PVALPDEIMGLSAKSV-WILLLFVYA 232

Query: 265 FIAAVLPVWLLLAPRDYLSTFLKIGTIIALAIGILIVAPELKMPAFTQFAKGGGPVWSGN 324
            IA++LPVW+LL PRDY++       +  L + +L  APEL  PAF Q      P    +
Sbjct: 233 AIASLLPVWVLLQPRDYINGLQLFVGLGLLYLAVLFGAPELVAPAFNQELPADTP----S 288

Query: 325 LFPFLFITIACGAVSGFHALISSGTTPKLLESEAHMRFIGYGAMLAESFVAIMALVAASV 384
           + P LF+TIACGA+SGFH L++SGTT K L+ E   RF+GY   + E  +++ A++  + 
Sbjct: 289 IVPLLFVTIACGAISGFHGLVASGTTSKQLDKETDARFVGYFGAMGEGMLSLAAIICCTA 348

Query: 385 IEPGVYFAMNSPAAVIGTSPESVAQVVSGWGFVITPD-VLIQTAKDVGENSIISRAGGAP 443
                                         GF    D   + TA   G  +   + GG  
Sbjct: 349 ------------------------------GFATLTDWQQVYTAFGSGGVTAFVQGGGT- 377

Query: 444 TLAVGIAHILHQVVGGQAMMAFWYHFAILFEALFILTAVDAGTRAGRFMLQ---DLLGSF 500
            LA G+   L   +GG  +       AILF      T +D G R  RF++Q   +L G  
Sbjct: 378 LLANGLG--LPAELGGTILAV----MAILFAG----TTMDTGLRLQRFVIQEAGELAGMK 427

Query: 501 VPSFRRTDSLPANLIATALTVSFWGYFLYQGVVDPLGGINTLWPLFGISNQMLAAVALVL 560
           V +          LI T + V       +    D  GG+  +WPLFG +NQ+LA + L +
Sbjct: 428 VNT----------LIGTLIAVGVCMALAFGAGSDGTGGM-VIWPLFGTTNQLLAGLTLAV 476

Query: 561 GTCVLVKMKRGQYAWVTLVPTIWLLICTLTA 591
            T +L+K+  G+    TLVP ++LL  ++ A
Sbjct: 477 ITVILIKL--GRSPLYTLVPLVFLLAMSIYA 505


Lambda     K      H
   0.328    0.141    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1121
Number of extensions: 66
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 690
Length of database: 558
Length adjustment: 37
Effective length of query: 653
Effective length of database: 521
Effective search space:   340213
Effective search space used:   340213
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources