Alignments for a candidate for ams in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for ams in Pseudomonas stutzeri RCH2

Align Alpha-glucosidase; EC 3.2.1.- (characterized, see rationale)
to candidate GFF3717 Psest_3786 Glycosidases

Query= uniprot:A8LLL3
         (552 letters)



>FitnessBrowser__psRCH2:GFF3717
          Length = 539

 Score =  201 bits (511), Expect = 6e-56
 Identities = 168/554 (30%), Positives = 251/554 (45%), Gaps = 57/554 (10%)

Query: 17  PDWWRGAVIYQIYPRSFQDSNGDGIGDLLGIVERMPYIASLGVDAIWISPFFTSPMKDFG 76
           P W+R A IYQI P  F+DSNGDG GDL GI ER+ YI  LG  A+W+ PF+ SP +D G
Sbjct: 3   PLWYRNAAIYQIDPTLFRDSNGDGCGDLRGITERLDYIRGLGCTAVWLMPFYQSPFEDAG 62

Query: 77  YDISDYFDVDPMFGSLADFDALIETAHMYGLRVMIDLVLSHTSDQHPWFEESRSSRDNPK 136
           YDISD+  VD  FG LAD  AL+E A   GL V+I+LV+ HTS QH WF+E+R  R++P 
Sbjct: 63  YDISDHLQVDERFGDLADIVALLEKAEELGLHVIIELVVQHTSIQHKWFQEARRDRNSPY 122

Query: 137 ADWYVWADAKPDGTPPNNWLSIFGGSGWHWDARRCQYYLHNFLTSQPDLNFHCADVQDAL 196
            D+Y+WAD   D   P         S W WD    QYY H F   +PDL+     V   +
Sbjct: 123 RDYYIWADEPDDFMEP--IFPTVEDSIWTWDEEAGQYYRHLFYKHEPDLDLTNPRVIHEI 180

Query: 197 LGVGRFWLDRGVDGFRLDTINFYVHDA-------------ELRDNPPLPPEERNSNIAPE 243
             +  FWL  GV GFR+D     V  A              +RD   +   E  + +  E
Sbjct: 181 ERIMSFWLRLGVSGFRIDAAVHMVRQAGGGELEKGYWLLEHMRDFVTMRRPE--TVLLGE 238

Query: 244 VNPYNHQRHLYSKNQPENLEFLAKFRAMMEEYPAIAAVGEVGDAQYGLEILGQYTRGETG 303
           ++    +   Y  ++ + +  L  F      + ++A       A+  +  L       + 
Sbjct: 239 IDTDPDKYVEYFGDEADRVTLLLDFWTNNHLFLSLAR----QQAEPLVRALNSQPLPPS- 293

Query: 304 VHMCYAFEFLAQEKLTAKRVAE-VLNKVDEVASDGWACWAFSNHDVMRHVSRWDLTPGAQ 362
            H  YA      ++L+  R+ E   N+V +  +      A+ N  + R ++   +  G +
Sbjct: 294 -HSQYALWIRNHDELSLDRLEEDERNEVMDTFAPDENMRAY-NRGIRRRLA--PMLDGDE 349

Query: 363 RGML---TLLMCLRGSVCLYQGEELGLPEAEVAFDDLQDPYGIEFWPEYKGRDGCRTPMV 419
           R +     LL  L G+  +  GEE+G+       DDL  P           R   RTPM 
Sbjct: 350 RRIAATHALLFSLPGTPIIRYGEEIGMG------DDLDRP----------ERLAVRTPMQ 393

Query: 420 WQSDNMSGGFSIHRPWL--PVSTE----HLGLAVAVQEEAPDALLHHYRRALAFRRAHPA 473
           W S+  + GFS  +  L  PV  E    +  + V  Q    D+L+      +  R     
Sbjct: 394 W-SNEPNAGFSCTKGELAAPVIDEGPFTYEKINVFAQTLRSDSLMARTGNMIRTRIGLRE 452

Query: 474 LVKGDISDVTVVGDVISFLRKDPEETVFVAINMSDAPGAVDLPPGNWM-QIGAELNSGGT 532
           +  G  + V V    +  +R D   TV + +N++D    V++   +    +    +    
Sbjct: 453 IGIGKRTTVEVNDPAVFAIRHDNGSTVLMLVNLADQETTVEITDDDLQDMVDVLADCDYD 512

Query: 533 SPDG---RVHLGPW 543
            P+G   ++ LGP+
Sbjct: 513 QPEGTPLKIRLGPY 526


Lambda     K      H
   0.321    0.138    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 905
Number of extensions: 42
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 552
Length of database: 539
Length adjustment: 35
Effective length of query: 517
Effective length of database: 504
Effective search space:   260568
Effective search space used:   260568
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources