Alignments for a candidate for pgmA in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for pgmA in Pseudomonas stutzeri RCH2

Align phosphomannomutase (EC 5.4.2.8) (characterized)
to candidate GFF3737 Psest_3806 Phosphomannomutase

Query= BRENDA::P26276
         (463 letters)



>FitnessBrowser__psRCH2:GFF3737
          Length = 857

 Score =  814 bits (2103), Expect = 0.0
 Identities = 397/463 (85%), Positives = 431/463 (93%)

Query: 1   MSTAKAPTLPASIFRAYDIRGVVGDTLTAETAYWIGRAIGSESLARGEPCVAVGRDGRLS 60
           MS+AKAP LPASIFRAYDIRGVVGD+LT ETAYW+GRAIGSESLA+GEP V+VGRDGRLS
Sbjct: 395 MSSAKAPNLPASIFRAYDIRGVVGDSLTTETAYWVGRAIGSESLAKGEPNVSVGRDGRLS 454

Query: 61  GPELVKQLIQGLVDCGCQVSDVGMVPTPVLYYAANVLEGKSGVMLTGSHNPPDYNGFKIV 120
           GPELV+ LIQGL+D GC VSD+GMVPTPVLYYAAN+L GKSGVMLTGSHNPPDYNGFKIV
Sbjct: 455 GPELVQHLIQGLLDSGCDVSDIGMVPTPVLYYAANILAGKSGVMLTGSHNPPDYNGFKIV 514

Query: 121 VAGETLANEQIQALRERIEKNDLASGVGSVEQVDILPRYFKQIRDDIAMAKPMKVVVDCG 180
           +AG+TLANEQIQ LR+RIE NDL+SGVG VEQVD+L RYF+QIR DIAMAKPMKVVVDCG
Sbjct: 515 IAGDTLANEQIQTLRKRIENNDLSSGVGKVEQVDVLERYFQQIRSDIAMAKPMKVVVDCG 574

Query: 181 NGVAGVIAPQLIEALGCSVIPLYCEVDGNFPNHHPDPGKPENLKDLIAKVKAENADLGLA 240
           NGVAGVIAP++IEALGC+VIPLYC+VDGNFPNHHPDPGKPENL DLIAKVK+E ADLGLA
Sbjct: 575 NGVAGVIAPRMIEALGCTVIPLYCDVDGNFPNHHPDPGKPENLVDLIAKVKSEKADLGLA 634

Query: 241 FDGDGDRVGVVTNTGTIIYPDRLLMLFAKDVVSRNPGADIIFDVKCTRRLIALISGYGGR 300
           FDGDGDRVGVVTN GT+IY DRLLMLFAKDVVSRNPGADIIFDVKCTRRL  LISGYGGR
Sbjct: 635 FDGDGDRVGVVTNAGTMIYADRLLMLFAKDVVSRNPGADIIFDVKCTRRLTPLISGYGGR 694

Query: 301 PVMWKTGHSLIKKKMKETGALLAGEMSGHVFFKERWFGFDDGIYSAARLLEILSQDQRDS 360
           PVMWKTGHSLIKKKMKE+GALLAGEMSGH+FFKERWFGFDDGIYSAARLLEILSQD+RD+
Sbjct: 695 PVMWKTGHSLIKKKMKESGALLAGEMSGHIFFKERWFGFDDGIYSAARLLEILSQDKRDA 754

Query: 361 EHVFSAFPSDISTPEINITVTEDSKFAIIEALQRDAQWGEGNITTLDGVRVDYPKGWGLV 420
           E VF+AFP DISTPEINITVTE+SKF I++ALQRDAQWGE N+ TLDGVRVDYPKGWGL+
Sbjct: 755 EQVFAAFPCDISTPEINITVTEESKFTIMDALQRDAQWGEANLITLDGVRVDYPKGWGLI 814

Query: 421 RASNTTPVLVLRFEADTEEELERIKTVFRNQLKAVDSSLPVPF 463
           RASNTTPVLVLRFEAD+EEEL RI+ VFR QL  V   L +PF
Sbjct: 815 RASNTTPVLVLRFEADSEEELSRIQDVFRAQLLNVAPDLKLPF 857


Lambda     K      H
   0.319    0.138    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1147
Number of extensions: 38
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 463
Length of database: 857
Length adjustment: 38
Effective length of query: 425
Effective length of database: 819
Effective search space:   348075
Effective search space used:   348075
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources