GapMind for catabolism of small carbon sources

 

Finding step braG for L-threonine catabolism in Pseudomonas stutzeri RCH2

5 candidates for braG: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 2 (BraG/NatE)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi Psest_1308 ABC-type branched-chain amino acid transport systems, ATPase component High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 83% 100% 382.9 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 70% 324.7
med Psest_3854 ABC-type branched-chain amino acid transport systems, ATPase component High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 44% 92% 183 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 44% 181.0
med Psest_0563 urea ABC transporter, ATP-binding protein UrtE High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 43% 93% 171.8 UrtE, component of The high-affinity (<1 μM) urea porter 48% 194.5
med Psest_1090 ABC-type branched-chain amino acid transport systems, ATPase component High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized) 40% 100% 166.8 high-affinity branched-chain amino acid ABC transporter, ATP-binding protein LivF 39% 156.4
lo Psest_1089 ABC-type branched-chain amino acid transport systems, ATPase component NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) 33% 87% 114.8 NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE 40% 179.9

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step braG

Or cluster all characterized braG proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory