Align Putative thymidine phosphorylase; EC 2.4.2.4; TdRPase (uncharacterized)
to candidate GFF3458 Psest_3523 putative thymidine phosphorylase
Query= curated2:Q21W90 (522 letters) >FitnessBrowser__psRCH2:GFF3458 Length = 526 Score = 774 bits (1999), Expect = 0.0 Identities = 390/509 (76%), Positives = 445/509 (87%) Query: 14 LVLRRVAIDTYRENVAYLHRDCAVYRAEGFQALSKVEVRANGRHILATLNVVDDPNIVAC 73 L LRRVAIDTYRENVAYLHRDCA RAEGFQAL+KVE+R NG+ ILA+LN+VDDP IV C Sbjct: 18 LALRRVAIDTYRENVAYLHRDCAACRAEGFQALAKVEIRVNGKRILASLNLVDDPAIVGC 77 Query: 74 NELGLSEDAFAQMAVIDGQPASVSQAEPPQSIGALRRKLAGERLGREDFLGIVRDIAELH 133 +ELGLSEDAFAQ+ G PAS+SQAEP SI AL RK+AGERL REDFL I+RDIAE Sbjct: 78 DELGLSEDAFAQLGAEAGVPASISQAEPAASIPALHRKIAGERLRREDFLAIMRDIAEHR 137 Query: 134 YSKIELSAFVVATNRDELDREEVYFLTEAMVASGRTLNWHEPLVVDKHCIGGIPGNRSSM 193 YSKIEL+AFVVA N+ ELDREEV+FLT+AM+ GR L+W E VVDKHCIGGIPGNR+SM Sbjct: 138 YSKIELTAFVVACNQGELDREEVFFLTDAMIQVGRRLDWREHPVVDKHCIGGIPGNRTSM 197 Query: 194 LVVPIVAAHGLLCPKTSSRAITSPAGTADTMEVLAKVELPVDQLADIVRTHRGCLAWGGA 253 LVVPIVAAHG+LCPKTSSRAITSPAGTADTMEVLA VELP ++L ++VR RGCLAWGG Sbjct: 198 LVVPIVAAHGMLCPKTSSRAITSPAGTADTMEVLANVELPFERLCEMVREERGCLAWGGT 257 Query: 254 AHLSPADDVLISVERPLAIDSPGQMVASILSKKIAAGSTHLVLDIPIGPSAKVRSMPEAQ 313 + LSPADDVLI+VERPL+IDSPGQMVASILSKKIAAGSTHL+LDIPIGP+AKVRSM EAQ Sbjct: 258 SELSPADDVLIAVERPLSIDSPGQMVASILSKKIAAGSTHLLLDIPIGPTAKVRSMAEAQ 317 Query: 314 RLRRLFEYVAGRMHLSLDVVVTDGRQPIGNGIGPVLEARDVMRVLENDPRAPNDLRQKSL 373 RLR+LFE+VA R+ L+LDVV+TDGRQPIGNGIGPVLEARDVM+VL+NDP AP DLRQK+L Sbjct: 318 RLRKLFEFVASRLGLTLDVVITDGRQPIGNGIGPVLEARDVMQVLQNDPAAPIDLRQKAL 377 Query: 374 RLAGRLIEFDPDVRGGDGFAIARDILDSGRALAKMNAIIAAQGAKPFDHNHPQLGALTFD 433 RLAGR++EFDPDVRGGDG+AIARDILDSGRAL KM++IIAAQG+KPFDHNHP L L+FD Sbjct: 378 RLAGRMLEFDPDVRGGDGYAIARDILDSGRALKKMHSIIAAQGSKPFDHNHPPLARLSFD 437 Query: 434 ICASESGVVTGIDNLQVARIARLAGAPKVIGAGIDLFHKLGEAVTSGEVLYRVHAGFQSD 493 + A S VVTGIDNLQ+ARIARLAGAPKV GAG+D+ KLGE V +G+ LYRV+A F +D Sbjct: 438 VLAERSAVVTGIDNLQLARIARLAGAPKVQGAGVDILRKLGEPVQAGDCLYRVYADFPAD 497 Query: 494 LDFARQACAKSTGYTLGRAEDVPHVFTEF 522 L FARQA +++G +LG A+ VP VF EF Sbjct: 498 LAFARQATERASGVSLGSADQVPSVFVEF 526 Lambda K H 0.320 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 912 Number of extensions: 30 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 522 Length of database: 526 Length adjustment: 35 Effective length of query: 487 Effective length of database: 491 Effective search space: 239117 Effective search space used: 239117 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
Align candidate GFF3458 Psest_3523 (putative thymidine phosphorylase)
to HMM TIGR02645 (putative thymidine phosphorylase (EC 2.4.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02645.hmm # target sequence database: /tmp/gapView.8866.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02645 [M=493] Accession: TIGR02645 Description: ARCH_P_rylase: putative thymidine phosphorylase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-187 610.4 0.0 1.3e-187 610.1 0.0 1.0 1 lcl|FitnessBrowser__psRCH2:GFF3458 Psest_3523 putative thymidine ph Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__psRCH2:GFF3458 Psest_3523 putative thymidine phosphorylase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 610.1 0.0 1.3e-187 1.3e-187 2 489 .. 19 511 .. 18 514 .. 0.98 Alignments for each domain: == domain 1 score: 610.1 bits; conditional E-value: 1.3e-187 TIGR02645 2 kvrvlnidtgqekvlinskd...lkeekltpqdrvevrlgkkslia..ivvssddlvesgevglseevveeleek 71 +r++ idt +e+v + ++d +++e++++ ++ve+r+++k ++a ++v++ +v +e+glse+++ +l ++ lcl|FitnessBrowser__psRCH2:GFF3458 19 ALRRVAIDTYRENVAYLHRDcaaCRAEGFQALAKVEIRVNGKRILAslNLVDDPAIVGCDELGLSEDAFAQLGAE 93 689*****************9999**********************887888889******************** PP TIGR02645 72 egdlvtvtpaekpeslrairkklrgkklkkeeikaivsdivdeklsdveisafltalaingldvdeiealtiamv 146 g ++++ae++ s++a+++k+ g++l++e+ ai++di+++++s +e++af++a++ + ld +e+ lt am+ lcl|FitnessBrowser__psRCH2:GFF3458 94 AGVPASISQAEPAASIPALHRKIAGERLRREDFLAIMRDIAEHRYSKIELTAFVVACNQGELDREEVFFLTDAMI 168 *************************************************************************** PP TIGR02645 147 etGetlewdrevivDkhsiGGvPGnktsllvvpivaaaGLliPktssraitsaaGtaDvvevltrvelsveelkr 221 + G l+w ++++vDkh+iGG+PGn+ts+lvvpivaa+G+l Pktssraits+aGtaD++evl++vel e l + lcl|FitnessBrowser__psRCH2:GFF3458 169 QVGRRLDWREHPVVDKHCIGGIPGNRTSMLVVPIVAAHGMLCPKTSSRAITSPAGTADTMEVLANVELPFERLCE 243 *************************************************************************** PP TIGR02645 222 ivekvggclvWGGalnlaPaDDvlikverpLslDpeeqllasilskkiaiGstkvliDiPvGpgakvksvkeaer 296 +v + gcl+WGG +l+PaDDvli verpLs+D+ q++asilskkia+Gst++l+DiP+Gp+akv+s+ ea+r lcl|FitnessBrowser__psRCH2:GFF3458 244 MVREERGCLAWGGTSELSPADDVLIAVERPLSIDSPGQMVASILSKKIAAGSTHLLLDIPIGPTAKVRSMAEAQR 318 *************************************************************************** PP TIGR02645 297 LakdlielgkrlgvtvevvityGsqPiGraiGPaLeakealavLesskeaPtsLvekslaLaaiLLemggaaerg 371 L+k+++++++rlg t +vvit+G+qPiG++iGP+Lea+++++vL+++++aP +L+ k+l+La+ +Le+++ ++ g lcl|FitnessBrowser__psRCH2:GFF3458 319 LRKLFEFVASRLGLTLDVVITDGRQPIGNGIGPVLEARDVMQVLQNDPAAPIDLRQKALRLAGRMLEFDPDVRGG 393 *************************************************************************** PP TIGR02645 372 aGkelarelLdsGkaleklkeiieaqGgdniksedievGklkadikaetdGyvteidnkaltriareaGaPedkg 446 G+++ar++LdsG+al+k++ ii aqG++ ++++ ++ +l++d+ ae++ +vt idn +l+riar+aGaP+ +g lcl|FitnessBrowser__psRCH2:GFF3458 394 DGYAIARDILDSGRALKKMHSIIAAQGSKPFDHNHPPLARLSFDVLAERSAVVTGIDNLQLARIARLAGAPKVQG 468 *************************************************************************** PP TIGR02645 447 aGvklhvkvgdkvkkGdplytiyaeseekldkaialaralepi 489 aGv + +k+g+ v++Gd ly++ya+ l a ++ ++ +++ lcl|FitnessBrowser__psRCH2:GFF3458 469 AGVDILRKLGEPVQAGDCLYRVYADFPADLAFARQATERASGV 511 ************************9998888887777766665 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (493 nodes) Target sequences: 1 (526 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.02 # Mc/sec: 9.94 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory