Align phosphopentomutase (EC 5.4.2.7) (characterized)
to candidate GFF945 Psest_0974 phosphoglucosamine mutase
Query= BRENDA::Q6I7B6 (450 letters) >FitnessBrowser__psRCH2:GFF945 Length = 445 Score = 143 bits (361), Expect = 1e-38 Identities = 100/269 (37%), Positives = 147/269 (54%), Gaps = 19/269 (7%) Query: 2 RLFGTAGIRGTLWEK-VTPELAMKVGMAVGTY--KSGKA--LVGRDGRTSSVMLKNAMIS 56 + FGT GIRG + + +TP+ +K+G A G K GK L+G+D R S M ++A+ + Sbjct: 4 KYFGTDGIRGHVGQAPITPDFMLKLGWAAGMAFRKQGKCRILIGKDTRISGYMFESALQA 63 Query: 57 GLLSTGMEVLDADLIPTPALAWGTRKL-ADAGVMITASHNPPTDNGVKVFNGDGTEFYVE 115 GL + G +VL +PTPA+A+ TR A+AG++I+ASHNP DNG+K F+G GT+ E Sbjct: 64 GLSAAGADVLLLGPMPTPAVAYLTRTFHAEAGIVISASHNPHHDNGIKFFSGRGTKLPDE 123 Query: 116 QERGLEE-------IIFSGNFRKARWDEIKPVRNVEVIPDYINAVLDFVGHETNLKVLYD 168 E +EE ++ S KA R +E + DF G LK++ D Sbjct: 124 VELMIEELIDAPMTVVESAQLGKASRINDAAGRYIEFCKSSVPTSTDFSG----LKLVID 179 Query: 169 GANGAGSLVAPYLLREMGAKVLSVNAHVDGHFPGRKPEPRYENIAYLGKLVRELGVDLAI 228 A+GA VAP + RE+GA+V+ + A DG + ++ L K V + G DL I Sbjct: 180 CAHGATYKVAPSVFRELGAEVVVIGAQPDG--LNINADVGSTHVGQLQKAVVDHGADLGI 237 Query: 229 AQDGDADRIAVFDEKGNYVDEDTVIALFA 257 A DGD DR+ + D G VD D ++ + A Sbjct: 238 AFDGDGDRVMMVDHTGAVVDGDELLYIIA 266 Lambda K H 0.318 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 469 Number of extensions: 27 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 450 Length of database: 445 Length adjustment: 33 Effective length of query: 417 Effective length of database: 412 Effective search space: 171804 Effective search space used: 171804 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory