Align trehalase (TreF;b3519) (EC 3.2.1.28) (characterized)
to candidate GFF357 Psest_0358 Neutral trehalase
Query= CAZy::AAC76544.1 (549 letters) >FitnessBrowser__psRCH2:GFF357 Length = 534 Score = 591 bits (1524), Expect = e-173 Identities = 293/515 (56%), Positives = 363/515 (70%), Gaps = 3/515 (0%) Query: 31 LDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDI 90 + ++ +P + + +D LTPA+RY ELF VQ IFPDSKTF DCAP P I Sbjct: 1 MTDIAPCQPFTYDVSAISLADTLTPAERYQELFVAVQMQCIFPDSKTFVDCAPLQHPEAI 60 Query: 91 LIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHI 150 L YR FDL FV HF L E+ E+V++P +SL EHID+LWP+LTR PQDH Sbjct: 61 LADYRGRCDEPGFDLAAFVREHFSLYEMPVKEFVANPDDSLAEHIDRLWPILTRHPQDHP 120 Query: 151 PWSSLLALPQSYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHI 210 SSLL LP Y+VPGGRF+E YYWDSYFTMLGL ESG DLL+ MADNFA++I+ YGH+ Sbjct: 121 EHSSLLPLPHDYVVPGGRFTELYYWDSYFTMLGLDESGHCDLLRAMADNFAYLIDTYGHV 180 Query: 211 PNGNRTYYLSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYR 270 PNGNRTYYL RSQPPVFALM ELFEE GV A YL L EYAFWMDGA++L P +A+R Sbjct: 181 PNGNRTYYLGRSQPPVFALMTELFEETGVYRASDYLPQLHKEYAFWMDGADALRPGEAHR 240 Query: 271 HVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSR 330 V + DG +LNRYWD+RDTPR+ES+ EDVETA+ S RP +EVYRDLRAGA SGWD+SSR Sbjct: 241 RCVCLADGVVLNRYWDERDTPREESYREDVETARSSCRPRHEVYRDLRAGAESGWDFSSR 300 Query: 331 WLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVN 390 WL D RLA+IRTT +P+DLNA L+KLE IA +SA+K + F ++A+AR A++ Sbjct: 301 WLDDAHRLATIRTTSILPVDLNALLYKLERQIAELSAVKNQHACAEDFARRAAARLSAID 360 Query: 391 RYLWDDENGIYRDYDWRR-EQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGI 449 YLW+ Y DYDW+R Q +AA + PL+VGMA+ EQA +A V++RLL PGG+ Sbjct: 361 NYLWNPNAAAYFDYDWQRGRQRDNLTAATLAPLFVGMASAEQAAAVALTVQARLLVPGGL 420 Query: 450 LASEY-ETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI 508 +E +GEQWD+PNGWAPLQW+ I+G + YG D L EI WL V+ + ++KL+ Sbjct: 421 ATTEIGGSGEQWDRPNGWAPLQWIGIRGLQRYGHDALALEIEERWLTIVSHLFERENKLV 480 Query: 509 EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLI 543 EKY + GGGEYPLQDGFGWTNGV R+L+ Sbjct: 481 EKY-VLRPCTEHAGGGEYPLQDGFGWTNGVTRKLM 514 Lambda K H 0.319 0.138 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1020 Number of extensions: 45 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 549 Length of database: 534 Length adjustment: 35 Effective length of query: 514 Effective length of database: 499 Effective search space: 256486 Effective search space used: 256486 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory