Aligments for a candidate for bch in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Aligments for a candidate for bch in Pseudomonas stutzeri RCH2

Align 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (characterized)
to candidate GFF2391 Psest_2439 Enoyl-CoA hydratase/carnithine racemase

Query= reanno::psRCH2:GFF2391
         (364 letters)



>FitnessBrowser__psRCH2:GFF2391
          Length = 364

 Score =  731 bits (1888), Expect = 0.0
 Identities = 364/364 (100%), Positives = 364/364 (100%)

Query: 1   MSVTERPVLAAVRNHVGHLTLNRPAGLNAVTLEMVRLLQQQLSAWAADPQIHAVVLRANG 60
           MSVTERPVLAAVRNHVGHLTLNRPAGLNAVTLEMVRLLQQQLSAWAADPQIHAVVLRANG
Sbjct: 1   MSVTERPVLAAVRNHVGHLTLNRPAGLNAVTLEMVRLLQQQLSAWAADPQIHAVVLRANG 60

Query: 61  EKAFCAGGDIRSLYDSFQRGDTEHETFFEEEYALDQYIHAYPKPLLALMDGFVLGGGMGL 120
           EKAFCAGGDIRSLYDSFQRGDTEHETFFEEEYALDQYIHAYPKPLLALMDGFVLGGGMGL
Sbjct: 61  EKAFCAGGDIRSLYDSFQRGDTEHETFFEEEYALDQYIHAYPKPLLALMDGFVLGGGMGL 120

Query: 121 VQGASLRVVTERVRMGMPEVGIGYFPDVGGSYFLSRLPGELGTYMGVTGLQIRAADALHV 180
           VQGASLRVVTERVRMGMPEVGIGYFPDVGGSYFLSRLPGELGTYMGVTGLQIRAADALHV
Sbjct: 121 VQGASLRVVTERVRMGMPEVGIGYFPDVGGSYFLSRLPGELGTYMGVTGLQIRAADALHV 180

Query: 181 GLADWCVSHDQIAELDRCLDRMSWSVHPQEALRTLVATLGSNKLPGSELKALHQAIDEHF 240
           GLADWCVSHDQIAELDRCLDRMSWSVHPQEALRTLVATLGSNKLPGSELKALHQAIDEHF
Sbjct: 181 GLADWCVSHDQIAELDRCLDRMSWSVHPQEALRTLVATLGSNKLPGSELKALHQAIDEHF 240

Query: 241 GKSDVVAIRASLAAETRPEFADWAEETVKVLDSRSPLAMCVTLEMLRRGRELPIADCFAL 300
           GKSDVVAIRASLAAETRPEFADWAEETVKVLDSRSPLAMCVTLEMLRRGRELPIADCFAL
Sbjct: 241 GKSDVVAIRASLAAETRPEFADWAEETVKVLDSRSPLAMCVTLEMLRRGRELPIADCFAL 300

Query: 301 ELHLDRQWFAKGDIMEGVRALIIDKDKSPRWNPPTLAEVTPERVQAFFDGFKATTGKARR 360
           ELHLDRQWFAKGDIMEGVRALIIDKDKSPRWNPPTLAEVTPERVQAFFDGFKATTGKARR
Sbjct: 301 ELHLDRQWFAKGDIMEGVRALIIDKDKSPRWNPPTLAEVTPERVQAFFDGFKATTGKARR 360

Query: 361 SATA 364
           SATA
Sbjct: 361 SATA 364


Lambda     K      H
   0.321    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 624
Number of extensions: 21
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 364
Length of database: 364
Length adjustment: 29
Effective length of query: 335
Effective length of database: 335
Effective search space:   112225
Effective search space used:   112225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources