Aligments for a candidate for praB in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Aligments for a candidate for praB in Pseudomonas fluorescens GW456-L13

Align 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized)
to candidate PfGW456L13_1083 Betaine aldehyde dehydrogenase (EC 1.2.1.8)

Query= metacyc::MONOMER-15108
         (486 letters)



>lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1083 Betaine
           aldehyde dehydrogenase (EC 1.2.1.8)
          Length = 490

 Score =  372 bits (956), Expect = e-107
 Identities = 197/476 (41%), Positives = 287/476 (60%), Gaps = 5/476 (1%)

Query: 14  FIDGKFVPSLDGKTFDNINPATEEKLGTVAEGGAAEIDLAVQAAKKALNGPWKKMTANER 73
           +IDG +  +    TF+ INPA  E L  V      +++ AV +A+K     W  MTA ER
Sbjct: 10  YIDGAYSDASSDATFEAINPANGEVLAKVQRATKEDVERAVVSAEKGQK-IWAAMTAMER 68

Query: 74  IAVLRKVGDLILERKEELSVLESLDTGKPTWLSGSIDIPRAAYNFHFFSDYIRTITNEAT 133
             +LR+  +++ ER +EL+ LE+LDTGK    +  +DI   A    +++  +  I  E  
Sbjct: 69  SRILRRAVEILRERNDELAALETLDTGKAYSETRYVDIVTGADVLEYYAGLVPAIEGEQI 128

Query: 134 QMDDVALNYAIRRPVGVIGLINPWNLPLLLMTWKLAPALAAGNTVVMKPAELTPMTATVL 193
            +   +  Y  R P+GV+  I  WN P+ +  WK APALAAGN ++ KP+E+T +T   L
Sbjct: 129 PLRTTSFVYTRREPLGVVAGIGAWNYPIQIALWKSAPALAAGNAMIFKPSEVTSLTTLKL 188

Query: 194 AEICRDAGVPDGVVNLVHGFGPNSAGAALTEHPDVNAISFTGETTTGKIIMASAAKT-LK 252
           AEI  +AGVP+GV N++ G G    G  LTEHP +  +SFTG T TGK +MASA+ + LK
Sbjct: 189 AEIYTEAGVPNGVFNVLTGSG-REVGTWLTEHPRIEKVSFTGGTDTGKKVMASASSSSLK 247

Query: 253 RLSYELGGKNPNVIFADSNLDEVIETTMKSSFINQGEVCLCGSRIYVERPAYEAFLEKFV 312
            ++ ELGGK+P +I  D++LD   +T M ++F + G+VC  G+R++V      AF  K  
Sbjct: 248 DVTMELGGKSPLIICDDADLDRAADTAMMANFYSSGQVCTNGTRVFVPAHLKAAFEAKIA 307

Query: 313 AKTKELVVGDPFDAKTKVGALISDEHYERVTGYIKLAVEEGGTILTGGKRPEGLE--KGY 370
            +   + +G+P D  T  G L+S  H E V GYI    EEG  +L GG+R    E  KG 
Sbjct: 308 ERVARIRIGNPEDENTNFGPLVSFAHMESVLGYIAKGKEEGARLLCGGERLTDGEFAKGA 367

Query: 371 FLEPTIITGLTRDCRVVKEEIFGPVVTVIPFDTEEEVLEQINDTHYGLSASVWTNDLRRA 430
           F+ PT+ T  T D  +V+EEIFGPV+ ++ ++TEEEV+ + NDT +GL+A + T DL RA
Sbjct: 368 FVAPTVFTDCTDDMTIVREEIFGPVMAILSYETEEEVIRRANDTDFGLAAGIVTKDLNRA 427

Query: 431 HRVAGQIEAGIVWVNTWFLRDLRTPFGGMKQSGIGREGGLHSFEFYSELTNICIKL 486
           HRV  Q+EAGI W+N W   D + P GG KQSG+GRE G+ S   ++ + ++ ++L
Sbjct: 428 HRVIHQLEAGICWINAWGESDAKMPVGGYKQSGVGRENGISSLNNFTRIKSVQVEL 483


Lambda     K      H
   0.318    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 633
Number of extensions: 28
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 486
Length of database: 490
Length adjustment: 34
Effective length of query: 452
Effective length of database: 456
Effective search space:   206112
Effective search space used:   206112
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources