Align ABC transporter for D-Alanine, permease component 1 (characterized)
to candidate PfGW456L13_445 Cystine ABC transporter, permease protein
Query= reanno::pseudo6_N2E2:Pf6N2E2_5404 (365 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_445 Length = 221 Score = 106 bits (264), Expect = 7e-28 Identities = 65/204 (31%), Positives = 112/204 (54%), Gaps = 6/204 (2%) Query: 157 GLMLTLVIATVGIVGALPLGIVLALGRRSNMPAIRVVCVTFIEFWRGVPLITVLFMSSVM 216 G T++++ G+ L +G LAL R S + + ++ F+RG PL+ LF+ Sbjct: 18 GAYYTVILSLGGMFFGLVMGFGLALMRLSRFKLVSWIARIYVSFFRGTPLLVQLFVIYYG 77 Query: 217 LPLFLPEGMNFDKLLRALIGVILFQSAYIAEVVRGGLQAIPKGQYEAAAAMGLGYWRSMG 276 LP G+ D L ALIG L +AY E++R + +I +GQ+EAAA++G+ +++ Sbjct: 78 LPQL---GLELDPLPAALIGFSLNMAAYACEILRAAISSIERGQWEAAASIGMTRAQTLR 134 Query: 277 LVILPQALKLVIPGIVNTFIALFKDTSLVIIIGLFDLLNSVKQAAADPKWLGMATEGYVF 336 ILPQA++ +P + N+FI+L KDT+L I + +L + A + + T Y+ Sbjct: 135 RAILPQAMRTALPPLGNSFISLVKDTALAATIQVPELFRQAQLITA--RTFEIFTM-YLA 191 Query: 337 AALVFWIFCFGMSRYSMHLERKLD 360 AAL++W+ +S LE +++ Sbjct: 192 AALIYWVLATVLSHLQNQLEARVN 215 Lambda K H 0.330 0.144 0.469 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 258 Number of extensions: 15 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 365 Length of database: 221 Length adjustment: 26 Effective length of query: 339 Effective length of database: 195 Effective search space: 66105 Effective search space used: 66105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory