Alignments for a candidate for dadA in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for dadA in Pseudomonas fluorescens GW456-L13

Align D-alanine dehydrogenase (EC 1.4.99.-) (characterized)
to candidate PfGW456L13_812 D-amino acid dehydrogenase small subunit (EC 1.4.99.1)

Query= reanno::psRCH2:GFF3724
         (432 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_812
          Length = 436

 Score =  745 bits (1923), Expect = 0.0
 Identities = 367/433 (84%), Positives = 398/433 (91%), Gaps = 1/433 (0%)

Query: 1   MRVLVLGSGVVGTASAYYLARAGFEVVVVDRQPAVAMETSFANAGQVSPGYASPWAAPGV 60
           MRV+VLGSGV+GT SAYYLARAGFEVVVVDRQPA AMETSFANAGQVSPGYASPWAAPGV
Sbjct: 3   MRVMVLGSGVIGTTSAYYLARAGFEVVVVDRQPAAAMETSFANAGQVSPGYASPWAAPGV 62

Query: 61  PLKAMKWLLQRHAPLAIKLTGDVDQYLWMAQMLRNCTAARYAVNKERMVRLSEYSRDCLD 120
           PLKA+KWLLQRHAPLAIK T D+DQYLWMAQMLRNCTA+RYAVNKERMVRLSEYSRDCLD
Sbjct: 63  PLKAIKWLLQRHAPLAIKATADIDQYLWMAQMLRNCTASRYAVNKERMVRLSEYSRDCLD 122

Query: 121 ELRAETGIAYEGRQLGTTQLFRTQAQLDAAAKDIAVLERSGVPYELLDRAAIGRVEPALA 180
           ELRAETGIAYEGR LGTTQLFRTQAQLD AAKDIAVL+ SGVP+ELLDR  I RVEPALA
Sbjct: 123 ELRAETGIAYEGRSLGTTQLFRTQAQLDGAAKDIAVLKESGVPFELLDREGIARVEPALA 182

Query: 181 KVAHKLSGALRLPNDQTGDCQMFTSRLAEMALALGVEFRFGQNIQRLEHAGDRIAGVWID 240
            V   L+GALRLPNDQTGDCQMFT++LAEMA  LGV+FRFGQ+IQRL+ AGDRI GVWID
Sbjct: 183 SVTDILAGALRLPNDQTGDCQMFTTKLAEMAANLGVQFRFGQDIQRLDFAGDRINGVWID 242

Query: 241 GKLETADRYVLALGSYSPQMLKPLGIRAPVYPLKGYSLTVPISDPAMAPQSTVLDETYKV 300
           GKLETADRYVLALGSYSPQ+LKPLGI+APVYPLKGYSLTVPI++PAMAP ST+LDETYKV
Sbjct: 243 GKLETADRYVLALGSYSPQLLKPLGIKAPVYPLKGYSLTVPITNPAMAPTSTILDETYKV 302

Query: 301 AITRFDQRIRVGGMAEIAGHDLSLNPRRRETLEMVVGDLYPQGGDPAEAVFWTGLRPATP 360
           AITRFD RIRVGGMAEIAG DLSLNPRRRETLEM+V DLYPQGG+ AEA FWTGLRP TP
Sbjct: 303 AITRFDNRIRVGGMAEIAGFDLSLNPRRRETLEMIVNDLYPQGGNLAEASFWTGLRPTTP 362

Query: 361 DGTPIIGATAYRNLYLNTGHGTLGWTMACGSGRVLADLLASKRPQISTDGLDIFRYG-KH 419
           DGTPI+GAT ++NL+LNTGHGTLGWTMACGSGR+LADL+A ++PQIS +GLDI RYG K 
Sbjct: 363 DGTPIVGATPFKNLFLNTGHGTLGWTMACGSGRLLADLMAKRKPQISAEGLDISRYGNKT 422

Query: 420 KETRKHAHPAAAH 432
           +E+ KH  PA AH
Sbjct: 423 QESAKHVSPAPAH 435


Lambda     K      H
   0.320    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 707
Number of extensions: 16
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 432
Length of database: 436
Length adjustment: 32
Effective length of query: 400
Effective length of database: 404
Effective search space:   161600
Effective search space used:   161600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources