Aligments for a candidate for dsdX in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Aligments for a candidate for dsdX in Pseudomonas fluorescens GW456-L13

Align D-serine transporter DsdX; D-serine-specific permease (characterized)
to candidate PfGW456L13_5135 Gluconate transporter family protein

Query= SwissProt::A0A0H2VAP9
         (445 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5135
          Length = 449

 Score =  310 bits (793), Expect = 8e-89
 Identities = 161/444 (36%), Positives = 261/444 (58%), Gaps = 6/444 (1%)

Query: 6   WVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMMGMGPLDMVNAIESGIGGTLGF 65
           W++    I+I+ +++ I +++ +PF+ + L S  +  + GM P  +V A E+G+G TLG 
Sbjct: 8   WLLVYAAIAIIALIVLIARYRLNPFIVITLISIGLALVAGMPPSGVVGAYEAGVGKTLGH 67

Query: 66  LAAVIGLGTILGKMMEVSGAAERIGLTL-QRCRWLSADVIMVLVGLICGITLFVEVGVVL 124
           +A V+ LGT+LGKMM  SG AE++  TL  R    +A   MV +  + G+ LF EVG VL
Sbjct: 68  IALVVALGTMLGKMMAESGGAEQVARTLIDRFGEKNAHWAMVTIAFLVGLPLFFEVGFVL 127

Query: 125 LIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPPHPAALYVANKLGADIGSVIVYGLL 184
           L+P+AF++A++   S+L + +P+   L  VH +VPPHPAA+       A +G  ++Y +L
Sbjct: 128 LVPIAFTVARRVGVSILMVGLPMVAGLSVVHALVPPHPAAMLAVQVYQASVGQTLMYAIL 187

Query: 185 VGLMASLIGGPLFLKFLGQRLPF---KPVPTEFADLKVRDEKTLPSLGATLFTVLLPIAL 241
           +G+  ++I GPL+ KF+  R+      P+  +F + + RD  +LP  G T+ T+LLP+ L
Sbjct: 188 IGIPTAIIAGPLYAKFIVPRIQLPAENPLERQFLEREPRD--SLPGFGITMATILLPVVL 245

Query: 242 MLVKTIAELNMARESGLYTLLEFIGNPITATFIAVFVAYYVLGIRQHMSMGTMLTHTENG 301
           ML+   A L     SG    L FIGN + A  +A  ++++ LG+ Q  +  ++L  T   
Sbjct: 246 MLIGGWANLISTPGSGFNQFLLFIGNSVIALLLATLLSFWTLGLAQGFNRESILKFTNEC 305

Query: 302 FGSIANILLIIGAGGAFNAILKSSSLADTLAVILSNMHMHPILLAWLVALILHAAVGSAT 361
               A+I L++GAGG  N IL  + + D +  +    H+ P+L+ WL A ++  A GSAT
Sbjct: 306 LAPTASITLLVGAGGGLNRILVDAGVTDQIVGLAHEFHLSPLLMGWLFAALMRIATGSAT 365

Query: 362 VAMMGATAIVAPMLPLYPDISPEIIAIAIGSGAIGCTIVTDSLFWLVKQYCGATLNETFK 421
           VAM  A+ +VAP+        PE++ +A G+G++  + V D  FWL+K+Y   T+ +TFK
Sbjct: 366 VAMTTASGVVAPVAIGLGYPHPELLVLATGAGSVIFSHVNDGGFWLIKEYFNMTVTQTFK 425

Query: 422 YYTTATFIASVIALAGTFLLSFII 445
            +T    + SV+A   T  LS++I
Sbjct: 426 TWTVLETLISVVAFGLTVGLSYLI 449


Lambda     K      H
   0.328    0.142    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 549
Number of extensions: 32
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 445
Length of database: 449
Length adjustment: 33
Effective length of query: 412
Effective length of database: 416
Effective search space:   171392
Effective search space used:   171392
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources