Alignments for a candidate for deh in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for deh in Pseudomonas fluorescens GW456-L13

Align Acetate/haloacid transporter, Dehp2, with a possible atypical topology (characterized)
to candidate PfGW456L13_2453 Major facilitator superfamily transporter

Query= TCDB::F8SVK1
         (552 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2453
          Length = 558

 Score =  781 bits (2017), Expect = 0.0
 Identities = 379/548 (69%), Positives = 462/548 (84%), Gaps = 3/548 (0%)

Query: 5   SGQISHAPMTKEEKRVIFASSLGTVFEWYDFYLAGSLAAFISKSFFSGVNPTAAFIFTLL 64
           +G   +  +T+EE++VIFASSLGTVFEWYDFYL GSLAA I+K FF+GVN T +FIF LL
Sbjct: 9   TGSTPNRGLTREERKVIFASSLGTVFEWYDFYLYGSLAAIIAKHFFAGVNETTSFIFALL 68

Query: 65  GFAAGFAVRPFGALVFGRLGDMVGRKYTFLITIVIMGLSTCVVGFLPGYAAIGMASPVIF 124
            FAAGFAVRPFGA+VFGRLGDM+GRK+TFLITIVIMG+ST VVG LPGYA IG+A+P+I 
Sbjct: 69  AFAAGFAVRPFGAIVFGRLGDMIGRKHTFLITIVIMGISTAVVGLLPGYATIGVAAPIIL 128

Query: 125 IAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGVRTAM 184
           I++RLLQGLALGGEYGGAATYVAEHAP  +RG++T+WIQTTATLGLFLSLLVIL  RTA+
Sbjct: 129 ISLRLLQGLALGGEYGGAATYVAEHAPKGKRGYFTSWIQTTATLGLFLSLLVILACRTAL 188

Query: 185 GEDAFGAWGWRIPFVASLVLLGISVWIRMQLHESPAFERIKAEGKTSKAPLSEAFGQWKN 244
           G +AF AWGWRIPF+ S++LL ISV+IR+QL+ESP F ++KAEGK+SKAPL+E+F +W+N
Sbjct: 189 GTEAFEAWGWRIPFLLSILLLIISVYIRLQLNESPVFVKMKAEGKSSKAPLTESFARWEN 248

Query: 245 LKIVILALIGVTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFG 304
           LKIVI+AL+G TAGQAVVWYTGQFYALFFL QTLK++  +AN+LIA +LLIGTPFF+ FG
Sbjct: 249 LKIVIMALLGGTAGQAVVWYTGQFYALFFLLQTLKIEPQTANLLIAGSLLIGTPFFVIFG 308

Query: 305 SLSDRIGRKPIILAGCLIAALTYFPLFKALTHYANPALEAATQKSPIVVIANPDECSFQF 364
           SLSDRIGRK II+AGC+IAALTYFP+F ALT Y NP +  A +K+P+ V+ANP +CSFQF
Sbjct: 309 SLSDRIGRKGIIMAGCIIAALTYFPIFHALTQYGNPDVFIAQEKNPVTVVANPAQCSFQF 368

Query: 365 NPVGTSKFTSSCDIAKSALSKAGLNYDNVAAPAGTLAQIKVGDTTIDTYDG---KAADAK 421
           +PVG +KFTSSCD+AK+ L+K  + Y N  A  G +AQ+++GD  I++++G    AAD K
Sbjct: 369 DPVGKAKFTSSCDLAKTVLAKRAIPYKNADAEPGAVAQVRIGDKVIESFEGTGMPAADFK 428

Query: 422 DAGKAFDKNLGTALKAASYPPKADPSQLNWPMTVVILTILVIYVTMVYGPIAAMLVEMFP 481
               AF   L TALK A YP KADP++ N+PM +++LTILVIYVTMVYGPIAA LVE+FP
Sbjct: 429 AKNDAFATTLATALKDAGYPEKADPAKTNYPMVLLLLTILVIYVTMVYGPIAAWLVELFP 488

Query: 482 TRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIIIALATFVIGLLFVR 541
            RIRYTSMSLPYHIGNGWFGGFLP  AFA+VAA G+IY GLWYPI+IA+ T V+G+ F+ 
Sbjct: 489 ARIRYTSMSLPYHIGNGWFGGFLPTVAFAMVAATGDIYYGLWYPIVIAVMTAVLGIFFMP 548

Query: 542 ETKDSNIY 549
           ETKD  I+
Sbjct: 549 ETKDREIH 556


Lambda     K      H
   0.325    0.139    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 899
Number of extensions: 39
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 552
Length of database: 558
Length adjustment: 36
Effective length of query: 516
Effective length of database: 522
Effective search space:   269352
Effective search space used:   269352
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources