GapMind for catabolism of small carbon sources

 

L-arabinose catabolism in Pseudomonas fluorescens GW456-L13

Best path

araF, araG, araH, xacB, xacC, xacD, xacE, xacF

Also see fitness data for the top candidates

Rules

Overview: L-arabinose utilization in GapMind is based on MetaCyc pathways L-arabinose degradation I, via xylulose 5-phosphate (link); III, oxidation to 2-oxoglutarate (link); and IV, via glycolaldehyde (link). Pathway II via xylitol and xylulose is not represented in GapMind because it is not reported in prokaryotes (link).

40 steps (21 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
araF L-arabinose ABC transporter, substrate-binding component AraF PfGW456L13_2120
araG L-arabinose ABC transporter, ATPase component AraG PfGW456L13_2121 PfGW456L13_3911
araH L-arabinose ABC transporter, permease component AraH PfGW456L13_2122
xacB L-arabinose 1-dehydrogenase PfGW456L13_2119 PfGW456L13_2058
xacC L-arabinono-1,4-lactonase PfGW456L13_2118
xacD L-arabinonate dehydratase PfGW456L13_3320 PfGW456L13_3725
xacE 2-dehydro-3-deoxy-L-arabinonate dehydratase PfGW456L13_3317 PfGW456L13_4553
xacF alpha-ketoglutarate semialdehyde dehydrogenase PfGW456L13_5044 PfGW456L13_3316
Alternative steps:
aldA (glycol)aldehyde dehydrogenase PfGW456L13_3932 PfGW456L13_495
aldox-large (glycol)aldehyde oxidoreductase, large subunit
aldox-med (glycol)aldehyde oxidoreductase, medium subunit
aldox-small (glycol)aldehyde oxidoreductase, small subunit PfGW456L13_3353 PfGW456L13_2091
araA L-arabinose isomerase
araB ribulokinase
araD L-ribulose-5-phosphate epimerase
araE L-arabinose:H+ symporter
araS L-arabinose ABC transporter, substrate-binding component AraS
araT L-arabinose ABC transporter, permease component 1 (AraT)
araU L-arabinose ABC transporter, permease component 2 (AraU)
araUsh L-arabinose ABC transporter, substrate-binding component AraU(Sh)
araV L-arabinose ABC transporter, ATPase component AraV PfGW456L13_3039 PfGW456L13_1897
araVsh L-arabinose ABC transporter, ATPase component AraV(Sh) PfGW456L13_2121 PfGW456L13_3911
araWsh L-arabinose ABC transporter, permease component 1 AraW(Sh) PfGW456L13_3910
araZsh L-arabinose ABC transporter, permease component 2 AraZ(Sh)
BT0355 L-arabinose:Na+ symporter
chvE L-arabinose ABC transporter, substrate-binding component ChvE
Echvi_1880 L-arabinose:Na+ symporter
gguA L-arabinose ABC transporter, ATPase component GguA PfGW456L13_3911 PfGW456L13_2121
gguB L-arabinose ABC transporter, permease component GguB
glcB malate synthase PfGW456L13_1139
gyaR glyoxylate reductase PfGW456L13_4945 PfGW456L13_2948
KDG-aldolase 2-dehydro-3-deoxy-L-arabinonate aldolase
xacG L-arabinose ABC transporter, substrate-binding component XacG
xacH L-arabinose ABC transporter, permease component 1 (XacH) PfGW456L13_1895
xacI L-arabinose ABC transporter, permease component 2 (XacI) PfGW456L13_1896
xacJ L-arabinose ABC transporter, ATPase component 1 (XacJ) PfGW456L13_1897 PfGW456L13_3039
xacK L-arabinose ABC transporter, ATPase component 2 (XacK) PfGW456L13_1897 PfGW456L13_3039
xylFsa L-arabinose ABC transporter, substrate-binding component XylF
xylGsa L-arabinose ABC transporter, ATPase component XylG PfGW456L13_483 PfGW456L13_2121
xylHsa L-arabinose ABC transporter, permease component XylH

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory