Align arginine deiminase (EC 3.5.3.6) (characterized)
to candidate PfGW456L13_1874 Arginine deiminase (EC 3.5.3.6)
Query= BRENDA::P13981 (418 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 Length = 418 Score = 718 bits (1853), Expect = 0.0 Identities = 343/418 (82%), Positives = 380/418 (90%) Query: 1 MSTEKTKLGVHSEAGKLRKVMVCSPGLAHQRLTPSNCDELLFDDVIWVNQAKRDHFDFVT 60 M+TEK K GVHSEAGKLRKVMVCSPGLAHQRLTP+NCDELLFDDV+WV QAKRDHFDFVT Sbjct: 1 MTTEKVKYGVHSEAGKLRKVMVCSPGLAHQRLTPNNCDELLFDDVLWVAQAKRDHFDFVT 60 Query: 61 KMRERGIDVLEMHNLLTETIQNPEALKWILDRKITADSVGLGLTSELRSWLESLEPRKLA 120 KMRER IDVLEMHNLLT+ + PEAL WIL+RK+TA+SVGLGL +E SWL SLEPRK+A Sbjct: 61 KMRERNIDVLEMHNLLTDIVAIPEALDWILERKVTANSVGLGLINETSSWLRSLEPRKIA 120 Query: 121 EYLIGGVAADDLPASEGANILKMYREYLGHSSFLLPPLPNTQFTRDTTCWIYGGVTLNPM 180 E+LIGGV+ DDLP+S G ++M+R++LG+SSF+LPPLPNTQFTRDTTCWIYGGVTLNPM Sbjct: 121 EFLIGGVSGDDLPSSFGGKAIEMFRDFLGNSSFILPPLPNTQFTRDTTCWIYGGVTLNPM 180 Query: 181 YWPARRQETLLTTAIYKFHPEFANAEFEIWYGDPDKDHGSSTLEGGDVMPIGNGVVLIGM 240 YWPARRQETLLTTAIYKFHPEF A+FEIWYGDPD DHG+STLEGGDVMPIGNGVVLIGM Sbjct: 181 YWPARRQETLLTTAIYKFHPEFTKADFEIWYGDPDLDHGNSTLEGGDVMPIGNGVVLIGM 240 Query: 241 GERSSRQAIGQVAQSLFAKGAAERVIVAGLPKSRAAMHLDTVFSFCDRDLVTVFPEVVKE 300 GERSSRQAI Q+A +LF A E+VIVAGLPKSRAAMHLDTVFSFCDRDLVT+FPEVV + Sbjct: 241 GERSSRQAIAQLAVNLFKNKAVEKVIVAGLPKSRAAMHLDTVFSFCDRDLVTIFPEVVNQ 300 Query: 301 IVPFSLRPDPSSPYGMNIRREEKTFLEVVAESLGLKKLRVVETGGNSFAAEREQWDDGNN 360 IV F+LRPD S G++IRREE +FL+ VA++L LK LRVVETGGNSFAAEREQWDDGNN Sbjct: 301 IVAFTLRPDESKAGGIDIRREETSFLDTVAQALNLKALRVVETGGNSFAAEREQWDDGNN 360 Query: 361 VVCLEPGVVVGYDRNTYTNTLLRKAGVEVITISASELGRGRGGGHCMTCPIVRDPIDY 418 VV +EPGVV+GYDRNTYTNTLLRKAGVEVITISA ELGRGRGGGHCMTCPI+RDPIDY Sbjct: 361 VVAVEPGVVIGYDRNTYTNTLLRKAGVEVITISAGELGRGRGGGHCMTCPIIRDPIDY 418 Lambda K H 0.319 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 672 Number of extensions: 14 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 418 Length of database: 418 Length adjustment: 32 Effective length of query: 386 Effective length of database: 386 Effective search space: 148996 Effective search space used: 148996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate PfGW456L13_1874 (Arginine deiminase (EC 3.5.3.6))
to HMM TIGR01078 (arcA: arginine deiminase (EC 3.5.3.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01078.hmm # target sequence database: /tmp/gapView.18897.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01078 [M=408] Accession: TIGR01078 Description: arcA: arginine deiminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-166 540.6 0.0 1.2e-166 540.4 0.0 1.0 1 lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 Arginine deiminase (EC 3.5.3.6) Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 Arginine deiminase (EC 3.5.3.6) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 540.4 0.0 1.2e-166 1.2e-166 3 408 .] 10 416 .. 8 416 .. 0.99 Alignments for each domain: == domain 1 score: 540.4 bits; conditional E-value: 1.2e-166 TIGR01078 3 vysEiGkLkkvllhrPgkelenltPsnldelLFddilavedakkeHdeFveklrd 57 v+sE+GkL+kv++++Pg ++++ltP+n+delLFdd+l+v +ak++H +Fv+k+r+ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 10 VHSEAGKLRKVMVCSPGLAHQRLTPNNCDELLFDDVLWVAQAKRDHFDFVTKMRE 64 99***************************************************** PP TIGR01078 58 ngvevllledLlaEtlaaseakeklldealeeaeilgeelkkelreylksletre 112 ++++vl++++Ll++ +a++ea +++l++++++++ +g +l +e +++l+sle+r+ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 65 RNIDVLEMHNLLTDIVAIPEALDWILERKVTANS-VGLGLINETSSWLRSLEPRK 118 **********************************.9******************* PP TIGR01078 113 lvekllaGvakeelpeeedselslvdlevegdsefvidPlPnllFtRDpsasign 167 ++e l++Gv ++lp++ + + + g+s+f+++PlPn++FtRD++++i++ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 119 IAEFLIGGVSGDDLPSSFGGKAIEMFRDFLGNSSFILPPLPNTQFTRDTTCWIYG 173 ******************************************************* PP TIGR01078 168 gvtlnkmalkaRqrEtlftelifkhHpkfanaevevyyd....rsekaslEGGDv 218 gvtln+m+++aR++Etl+t++i+k+Hp+f++a++e++y+ ++ +++lEGGDv lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 174 GVTLNPMYWPARRQETLLTTAIYKFHPEFTKADFEIWYGdpdlDHGNSTLEGGDV 228 **************************************9999999********** PP TIGR01078 219 lvlskdvlviGiseRtsaqsveklakslfkneaefkkvlaiklpkkralmHLDtv 273 + ++++v++iG++eR+s+q++ +la +lfkn ++++kv+++ lpk+ra+mHLDtv lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 229 MPIGNGVVLIGMGERSSRQAIAQLAVNLFKN-KAVEKVIVAGLPKSRAAMHLDTV 282 *******************************.99********************* PP TIGR01078 274 ltmvDkdkftvfpevekelkafsiysltkgneekikveekakleevlaealgvkk 328 ++++D+d +t+fpev+++++af+++ ++++ + +++e++++ + +a+al++k lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 283 FSFCDRDLVTIFPEVVNQIVAFTLRPDESKAGGIDIRREETSFLDTVAQALNLKA 337 ******************************99999******************** PP TIGR01078 329 lkliktgGgdsveaeREqwnDgnnvlaiaPGvvvaYeRnvvtnallekaGikvlt 383 l++++tg g+s++aeREqw+Dgnnv+a++PGvv++Y+Rn++tn+ll+kaG++v+t lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 338 LRVVETG-GNSFAAEREQWDDGNNVVAVEPGVVIGYDRNTYTNTLLRKAGVEVIT 391 ******9.9********************************************** PP TIGR01078 384 iagseLsrGrGgprCmsmplvRddi 408 i++ eL+rGrGg++Cm++p+ Rd+i lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1874 392 ISAGELGRGRGGGHCMTCPIIRDPI 416 **********************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (408 nodes) Target sequences: 1 (418 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 9.35 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory