GapMind for catabolism of small carbon sources

 

Aligments for a candidate for arcB in Pseudomonas fluorescens GW456-L13

Align Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 (uncharacterized)
to candidate PfGW456L13_1016 Aspartate carbamoyltransferase (EC 2.1.3.2)

Query= curated2:Q2G3J8
         (311 letters)



>lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1016 Aspartate
           carbamoyltransferase (EC 2.1.3.2)
          Length = 339

 Score =  115 bits (288), Expect = 1e-30
 Identities = 93/313 (29%), Positives = 149/313 (47%), Gaps = 28/313 (8%)

Query: 5   LKHFLNLTDAGGDAIAAMLNDALDRKSARAGQPKGKADADAPL-GGRVLAMVFEKNSTRT 63
           L+HFL+L     + +  +L+ A       A     +A    PL  G+ +  VF +NSTRT
Sbjct: 18  LRHFLSLDGLRRELLTEILDTADSFLEVGA-----RAVKKVPLLRGKTVCNVFFENSTRT 72

Query: 64  RVSFDIAMRQLGGSALILDAGTTQLGRGETIADTARVLSRM-ADAIMLRTDDHAKVEELA 122
           R +F++A ++L    + L+  T+   +GET+ DT R L  M AD  ++R  D      +A
Sbjct: 73  RTTFELAAQRLSADVITLNVSTSSASKGETLLDTLRNLEAMAADMFVVRHGDSGAAHFIA 132

Query: 123 RHA--TVPVINGLTDL-SHPCQIMADLLTVIEHGKALPGLEVAWLGDGNNVLNSIVEAAG 179
            H    V +ING     +HP Q M D+LT+  H      L VA +GD   +L+S V  + 
Sbjct: 133 EHVCPQVAIINGGDGRHAHPTQGMLDMLTIRRHKGGFENLSVAIVGD---ILHSRVARSN 189

Query: 180 LMKFNVRIGVPEGYESDAGMIEAAVLAGAGIRVIRDPVEAVRGADVVVTDTWISMGQAHA 239
           ++     +G P+        +    +   G++V  D  E ++  DVV+      M +   
Sbjct: 190 MLALKT-LGCPDIRVIAPKTLLPIGIEQYGVKVYTDMTEGLKDVDVVI------MLRLQR 242

Query: 240 EAKLAAMAP--------YQVNDALMAHAKPDAVFLHCLPAHRGEEVTDAVIDGPRSVVWD 291
           E     + P        + +  A +A AKPDA+ +H  P +RG E+  AV DGP SV+ +
Sbjct: 243 ERMTGGLLPSEGEFYRLFGLTTARLAGAKPDAIVMHPGPINRGVEIESAVADGPHSVILN 302

Query: 292 EAENRIHAQKSVL 304
           +    I  + +VL
Sbjct: 303 QVTYGIAIRMAVL 315


Lambda     K      H
   0.319    0.133    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 234
Number of extensions: 18
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 311
Length of database: 339
Length adjustment: 28
Effective length of query: 283
Effective length of database: 311
Effective search space:    88013
Effective search space used:    88013
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory