Align Ornithine carbamoyltransferase 1, anabolic; OTCase 1; Ornithine carbamoyltransferase 1, phaseolotoxin-sensitive; SOCT; EC 2.1.3.3 (characterized)
to candidate PfGW456L13_1739 Ornithine carbamoyltransferase (EC 2.1.3.3)
Query= SwissProt::Q02047 (306 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1739 Length = 307 Score = 537 bits (1384), Expect = e-157 Identities = 268/306 (87%), Positives = 284/306 (92%) Query: 1 MNARHFLSMMDYTPDELLGLIRRGVELKDLRIRGELFEPLKNRVLGMIFEKSSTRTRLSF 60 M+ARHFLS+MD TP+EL+ +IRRGVELKDLR RG LFEPLKNRVLGMIFEKSSTRTR+SF Sbjct: 2 MSARHFLSLMDCTPEELVSVIRRGVELKDLRNRGVLFEPLKNRVLGMIFEKSSTRTRISF 61 Query: 61 EAGMIQLGGQAIFLSHRDTQLGRGEPIADSAKVMSRMLDAVMIRTYAHSNLTEFAANSRV 120 EAGMIQLGGQAIFLS RDTQLGRGEPI D A VMS MLDAVMIRT+AHSNLTEFAANSRV Sbjct: 62 EAGMIQLGGQAIFLSPRDTQLGRGEPIGDCAIVMSSMLDAVMIRTFAHSNLTEFAANSRV 121 Query: 121 PVINGLSDDLHPCQLLADMQTFLEHRGSIKGKTVAWIGDGNNMCNSYIEAAIQFDFQLRV 180 PVINGLSDDLHPCQLLADMQTFLEHRGSI+GKTVAWIGDGNNMCNSYIEAAIQFDFQLRV Sbjct: 122 PVINGLSDDLHPCQLLADMQTFLEHRGSIQGKTVAWIGDGNNMCNSYIEAAIQFDFQLRV 181 Query: 181 ACPAGYEPNPEFLALAGERVTIVRDPKAAVAGAHLVSTDVWTSMGQEEETARRMALFAPF 240 ACP GYEPNP FLA AG+RVTIVRDPK AV GAHLVSTDVWTSMGQEEETA+R+ LFAP+ Sbjct: 182 ACPEGYEPNPVFLAKAGDRVTIVRDPKEAVRGAHLVSTDVWTSMGQEEETAKRLQLFAPY 241 Query: 241 QVTRASLDLAEKDVLFMHCLPAHRGEEISVDLLDDSRSVAWDQAENRLHAQKALLEFLVA 300 QV RA LDLA +DVLFMHCLPAHRGEEIS+DLLDD RSVAWDQAENRLHAQKALLEFLV Sbjct: 242 QVNRALLDLAAEDVLFMHCLPAHRGEEISIDLLDDKRSVAWDQAENRLHAQKALLEFLVE 301 Query: 301 PSHQRA 306 P++ A Sbjct: 302 PAYHHA 307 Lambda K H 0.322 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 377 Number of extensions: 5 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 306 Length of database: 307 Length adjustment: 27 Effective length of query: 279 Effective length of database: 280 Effective search space: 78120 Effective search space used: 78120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 48 (23.1 bits)
Align candidate PfGW456L13_1739 (Ornithine carbamoyltransferase (EC 2.1.3.3))
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00658.hmm # target sequence database: /tmp/gapView.31145.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00658 [M=304] Accession: TIGR00658 Description: orni_carb_tr: ornithine carbamoyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.4e-117 377.4 0.0 2.7e-117 377.3 0.0 1.0 1 lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1739 Ornithine carbamoyltransferase ( Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1739 Ornithine carbamoyltransferase (EC 2.1.3.3) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 377.3 0.0 2.7e-117 2.7e-117 1 303 [. 5 300 .. 5 301 .. 0.99 Alignments for each domain: == domain 1 score: 377.3 bits; conditional E-value: 2.7e-117 TIGR00658 1 rhllslldlseeelkellelakklkkekkkgkeekklkgktlaliFekrstRtRv 55 rh+lsl+d ++eel++++ ++ +lk +++g+ + lk++ l++iFek+stRtR+ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1739 5 RHFLSLMDCTPEELVSVIRRGVELKDLRNRGVLFEPLKNRVLGMIFEKSSTRTRI 59 8****************************************************** PP TIGR00658 56 sfevaayelGaqvlylnkeelqlgrkesikDtarvlsryvdaivvRvykhedvee 110 sfe+++++lG+q+++l++ ++qlgr+e+i D a v+s+++da+++R+++h++++e lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1739 60 SFEAGMIQLGGQAIFLSPRDTQLGRGEPIGDCAIVMSSMLDAVMIRTFAHSNLTE 114 ******************************************************* PP TIGR00658 111 lakyasvPvingLtdlehPcqilaDlltikeklgklkevklvyvGDannvansll 165 +a ++ vPvingL+d hPcq+laD++t e+ g++++ +++++GD+nn++ns + lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1739 115 FAANSRVPVINGLSDDLHPCQLLADMQTFLEHRGSIQGKTVAWIGDGNNMCNSYI 169 ******************************************************* PP TIGR00658 166 laaaklGldvvvatPeglepeaeivkkakkiakenggkleltedpkkavkdadvi 220 aa ++ ++++va+Peg+ep++ + ka g ++++++dpk+av++a+++ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1739 170 EAAIQFDFQLRVACPEGYEPNPVFLAKA-------GDRVTIVRDPKEAVRGAHLV 217 **********************998887.......88****************** PP TIGR00658 221 ytDvwvsmGeeekkeerlkllkpyqvneellelakpevkflhCLPavrGeevtde 275 tDvw+smG+ee++++rl+l+ pyqvn+ ll+la ++v f+hCLPa+rGee++ + lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1739 218 STDVWTSMGQEEETAKRLQLFAPYQVNRALLDLAAEDVLFMHCLPAHRGEEISID 272 ******************************************************* PP TIGR00658 276 vlegeasivfdeaenRlhaqkavlkall 303 l+ ++s+ +d+aenRlhaqka+l++l+ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1739 273 LLDDKRSVAWDQAENRLHAQKALLEFLV 300 ************************9987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (304 nodes) Target sequences: 1 (307 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 7.59 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory