Aligments for a candidate for yveA in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Aligments for a candidate for yveA in Pseudomonas fluorescens GW456-L13

Align Aspartate-proton symporter; L-aspartate transporter (characterized)
to candidate PfGW456L13_3582 Aspartate-proton symporter

Query= SwissProt::O07002
         (520 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3582
          Length = 548

 Score =  673 bits (1736), Expect = 0.0
 Identities = 301/512 (58%), Positives = 410/512 (80%), Gaps = 1/512 (0%)

Query: 1   MSKQGNFQKSMSLFDLILIGMGAIFGSAWLFAVSNVASKAGPSGAFSWILGGAIILLIGL 60
           MS QG F+K +SL DL  IG+GAIFGS WLFA S+V++ AGP+G FSW+LGG  +LL+G+
Sbjct: 1   MSGQGKFKKQLSLIDLTFIGLGAIFGSGWLFAASHVSAIAGPAGIFSWLLGGFAVLLLGI 60

Query: 61  VYAELGAALPRTGGIIRYPVYSHGHLVGYLISFVTIVAYTSLISIEVTAVRQYVAYWFPG 120
           VY ELGAALPR GG++RYPVYSHG L+GYL+ F+T++A++SL++IEV A RQY A WFP 
Sbjct: 61  VYCELGAALPRAGGVVRYPVYSHGPLLGYLMGFITLIAFSSLVAIEVVAARQYAAAWFPA 120

Query: 121 LTIKGSDSPTISGWILQFALLCLFFLLNYWSVKTFAKANFIISIFKYIVPITIIIVLIFH 180
           LT  GS +PT  GW++QF LLCLFF+LNY+SVKTFAKAN +IS+FK+IVP+ +I VL   
Sbjct: 121 LTQAGSSNPTAIGWLVQFGLLCLFFMLNYYSVKTFAKANNLISMFKFIVPLLVIGVLFTF 180

Query: 181 FQPENLSVQGFAPFGFTGIQAAISTGGVMFAYLGLHPIVSVAGEVQNPKRNIPIALIICI 240
           F+PEN   QGF PFG +G++ A+S GG++FAYLGL PI+SVA EV+NP+R IPIALI+ +
Sbjct: 181 FKPENFQSQGFMPFGLSGVEMAVSAGGIIFAYLGLTPIISVASEVKNPQRTIPIALILSV 240

Query: 241 IVSTIIYTVLQVTFIGAIPTETLKHGWPAIGREFSLPFKDIAVMLGLGWLATLVILDAIL 300
           ++ST IY +LQV F+G +PTE L +GW  + +E +LP++DIA+ LG+GWLA LV+ DA++
Sbjct: 241 LLSTAIYVLLQVAFLGGVPTEMLANGWAGVAKELALPYRDIALALGVGWLAYLVVADAVI 300

Query: 301 SPGGNGNIFMNTTSRLVYAWARNGTLFGIFSKVNKDTGTPRASLWLSFALSIFWTLPFPS 360
           SP G GNI+MN T R++Y WA+ GT F +F+++++ +G PR +LWL+FALS+FWTLPFPS
Sbjct: 301 SPSGCGNIYMNATPRVIYGWAQTGTFFKVFTRIDEKSGIPRPALWLTFALSVFWTLPFPS 360

Query: 361 WNALVNVCSVALILSYAIAPISSAALRVNAKDLNRPFYLKGMSIIGPLSFIFTAFIVYWS 420
           W AL+NV S AL+LSYA+AP+S AALR NA D+ RPF +K M ++GP+SFI  A IVYWS
Sbjct: 361 WEALINVVSAALVLSYAVAPVSVAALRRNAPDMPRPFKVKRMGVLGPVSFIIAALIVYWS 420

Query: 421 GWKTVSWLLGSQLVMFLIYLCFSKYTPKEDVSLAQQLKSAWWLIGFYIMMLIFSYIGSFG 480
           GW TVSWLLG Q++MF++YL   ++ P + +SLAQQ++S+ WLIGFY + ++ S++GSFG
Sbjct: 421 GWSTVSWLLGLQILMFVVYLLCGRFVPTQHLSLAQQVRSSAWLIGFYAVTIVLSWLGSFG 480

Query: 481 HGLGIISNPVDLILVAIGSLAIYYWAKYTGLP 512
            G+G++++P D ++VA  ++ IYYW   TG+P
Sbjct: 481 -GMGVLTHPFDTVVVAACAMGIYYWGAATGVP 511


Lambda     K      H
   0.328    0.143    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1043
Number of extensions: 47
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 548
Length adjustment: 35
Effective length of query: 485
Effective length of database: 513
Effective search space:   248805
Effective search space used:   248805
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources