Align phosphomannose mutase (EC 5.4.2.8) (characterized)
to candidate PfGW456L13_5092 Phosphoglucosamine mutase (EC 5.4.2.10)
Query= metacyc::MONOMER-13382 (455 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5092 Length = 445 Score = 205 bits (521), Expect = 3e-57 Identities = 148/445 (33%), Positives = 223/445 (50%), Gaps = 21/445 (4%) Query: 3 KLFGTFGVRGIANE-KITPEFAMKIGMAFGTLLKREGRKKPLVVVGRDTRVSGEMLKEAL 61 K FGT G+RG E ITP+F +K+G A G ++ G K V+VG+DTR+SG M + AL Sbjct: 4 KYFGTDGIRGRVGEYPITPDFMLKLGWAAGMAFRKMGACK--VLVGKDTRISGYMFESAL 61 Query: 62 ISGLLSVGCDVIDVGIAPTPAVQWATKHFNADGGAVITASHNPPEYNGIKLLEPNGMGLK 121 +GL S G DV+ +G PTPA+ + T+ F+A+ G VI+ASHNP + NGIK G L Sbjct: 62 EAGLTSAGADVMLLGPMPTPAIAYLTRTFHAEAGIVISASHNPHDDNGIKFFSGQGTKLP 121 Query: 122 KEREAIVEELFFKEDFDRAKWYEIGEVRR-EDIIKPYIEAIKSKVDVEAIKKRKPFVVVD 180 E E ++EEL + +IG+V R D YIE KS V +V+D Sbjct: 122 DEVEHMIEEL-LDTPMTVVESSKIGKVSRINDASGRYIEFCKSSVPT-GTSFTGLKIVID 179 Query: 181 TSNGAGSLTLPYLLRELGCKVITVNAQPDGYFPARNPEPNEENLKEFMEIVKALGADFGV 240 ++GA P + RELG +V+ ++AQP+G N ++ V A AD G+ Sbjct: 180 CAHGATYKVAPSVFRELGAEVVVLSAQPNGL--NINDNCGSTHMGSLQAAVVAEKADLGI 237 Query: 241 AQDGDADRAVFIDENGRFIQGDKTFALVADAVLKEKG---GGLLVTTVATSNLLDDIAKK 297 A DGD DR +D G + GD+ ++A L E+G GG++ T ++ L +A Sbjct: 238 AFDGDGDRVQMVDHTGAVVDGDELLFIIA-RDLHERGKLQGGVVGTLMSNLGLELALADL 296 Query: 298 HGAKVMRTKVGDLIVARALYENNGTIGGEENGGVIFPEHVLGRDGAMTVAKVVEIFAKSG 357 G ++R VGD V L E N +GGE +G ++ H D + +V+ + Sbjct: 297 -GIPLVRANVGDRYVIAELLERNWIVGGENSGHIVCFSHTTTGDAIIAALQVLMALGRRD 355 Query: 358 KKFSELIDELPKYYQIKTKRHVEGDRHAIVNKVAEMARERGYTVDTTDGAKIIFEDGWVL 417 + ++ L K Q+ G + + + A ER K + G VL Sbjct: 356 EGLAQSRQALRKCPQVLINVRFGGGASPLEHPSVKEASER--------VTKAMAGRGRVL 407 Query: 418 VRASGTEPIIRIFSEAKSKEKAQEY 442 +R SGTEP++R+ E + + + + Y Sbjct: 408 LRKSGTEPLVRVMVEGEDETQVRGY 432 Lambda K H 0.317 0.138 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 481 Number of extensions: 31 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 455 Length of database: 445 Length adjustment: 33 Effective length of query: 422 Effective length of database: 412 Effective search space: 173864 Effective search space used: 173864 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory