Align Isocitrate dehydrogenase [NADP]; IDH; Oxalosuccinate decarboxylase; EC 1.1.1.42 (characterized)
to candidate PfGW456L13_2641 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42)
Query= SwissProt::P16100
(741 letters)
>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641
Length = 741
Score = 1196 bits (3093), Expect = 0.0
Identities = 601/736 (81%), Positives = 659/736 (89%), Gaps = 1/736 (0%)
Query: 5 KIIYTLTDEAPALATYSLLPIIKAFTGSSGIAVETRDISLAGRLIATFPEYLTDTQKISD 64
KIIYT TDEAPALATYSLLPI++AFT S+ IAVETRDISLAGR++A+FPE L D + ++D
Sbjct: 6 KIIYTFTDEAPALATYSLLPIVEAFTASADIAVETRDISLAGRILASFPEQLGD-KAVAD 64
Query: 65 DLAELGKLATTPDANIIKLPNISASVPQLKAAIKELQQQGYKLPDYPEEPKTDTEKDVKA 124
LAELG LA TP+ANIIKLPNISASVPQL+AAIKELQ QGY LPDYPE D +K+ KA
Sbjct: 65 HLAELGALAVTPEANIIKLPNISASVPQLQAAIKELQAQGYALPDYPETVTCDADKEAKA 124
Query: 125 RYDKIKGSAVNPVLREGNSDRRAPLSVKNYARKHPHKMGAWSADSKSHVAHMDNGDFYGS 184
RYDKIKGSAVNPVLREGNSDRRAPLSVKNYARKHPHKMGAW+ DSKSH+AHM GDFYGS
Sbjct: 125 RYDKIKGSAVNPVLREGNSDRRAPLSVKNYARKHPHKMGAWAKDSKSHIAHMSTGDFYGS 184
Query: 185 EKAALIGAPGSVKIELIAKDGSSTVLKAKTSVQAGEIIDSSVMSKNALRNFIAAEIEDAK 244
EKAALI A +VKIELIA+DG++TVLK KTSVQAGEI+D +V+SKNALR+FIAAEIEDAK
Sbjct: 185 EKAALIDAADAVKIELIAQDGTATVLKEKTSVQAGEILDCAVLSKNALRSFIAAEIEDAK 244
Query: 245 KQGVLLSVHLKATMMKVSDPIMFGQIVSEFYKDALTKHAEVLKQIGFDVNNGIGDLYARI 304
QGVLLSVHLKATMMKVSDPIMFGQIV+EFYKDAL KHA+VL QIGF++NNGIGDLYARI
Sbjct: 245 AQGVLLSVHLKATMMKVSDPIMFGQIVAEFYKDALAKHADVLSQIGFNLNNGIGDLYARI 304
Query: 305 KTLPEAKQKEIEADIQAVYAQRPQLAMVNSDKGITNLHVPSDVIVDASMPAMIRDSGKMW 364
K LP +Q +IEADIQAVYA RP LAMVNSDKGITNLHVPSDVIVDASMPAMIRDSGKMW
Sbjct: 305 KALPAEQQAQIEADIQAVYAVRPSLAMVNSDKGITNLHVPSDVIVDASMPAMIRDSGKMW 364
Query: 365 GPDGKLHDTKAVIPDRCYAGVYQVVIEDCKQHGAFDPTTMGSVPNVGLMAQKAEEYGSHD 424
G DG+LHDTKAVIPDRCYA +YQ VIEDCK +GAFDPTTMGSVPNVGLMA+KAEEYGSHD
Sbjct: 365 GTDGQLHDTKAVIPDRCYATIYQAVIEDCKANGAFDPTTMGSVPNVGLMAKKAEEYGSHD 424
Query: 425 KTFQIPADGVVRVTDESGKLLLEQSVEAGDIWRMCQAKDAPIQDWVKLAVNRARATNTPA 484
KTFQI ADGVVRVTD G LL+EQ+VEAGDI+RMCQ KDAPIQDWVKLAVNRARA++TPA
Sbjct: 425 KTFQIKADGVVRVTDSKGNLLMEQAVEAGDIFRMCQTKDAPIQDWVKLAVNRARASSTPA 484
Query: 485 VFWLDPARAHDAQVIAKVERYLKDYDTSGLDIRILSPVEATRFSLARIREGKDTISVTGN 544
+FWLDP RAHD VI KV+ YLKD+DT+GLDI+I++PV+A + +L R R GKDTISVTGN
Sbjct: 485 IFWLDPMRAHDGVVIEKVQAYLKDHDTAGLDIQIMAPVDAMKSTLQRTRAGKDTISVTGN 544
Query: 545 VLRDYLTDLFPIMELGTSAKMLSIVPLMSGGGLFETGAGGSAPKHVQQFLEEGYLRWDSL 604
VLRDYLTDLFPIMELGTSAKMLSIVPLM+GGGLFETGAGGSAPKHVQQ LEE +LRWDSL
Sbjct: 545 VLRDYLTDLFPIMELGTSAKMLSIVPLMNGGGLFETGAGGSAPKHVQQLLEENFLRWDSL 604
Query: 605 GEFLALAASLEHLGNAYKNPKALVLASTLDQATGKILDNNKSPARKVGEIDNRGSHFYLA 664
GEFLALAASLEHLG Y NPKALVLA TLDQATG+ LDNNKSP+RKVG IDNRGSHFYLA
Sbjct: 605 GEFLALAASLEHLGVTYNNPKALVLAKTLDQATGQFLDNNKSPSRKVGNIDNRGSHFYLA 664
Query: 665 LYWAQALAAQTEDKELQAQFTGIAKALTDNETKIVGELAAAQGKPVDIAGYYHPNTDLTS 724
LYWAQALAAQTED LQAQF +AK LT+NE IV EL A QGKPVDI GYYH + +L S
Sbjct: 665 LYWAQALAAQTEDAALQAQFATLAKTLTENEATIVAELNAVQGKPVDIGGYYHADAELIS 724
Query: 725 KAIRPSATFNAALAPL 740
KA+RPSATFNAA+A L
Sbjct: 725 KAMRPSATFNAAVAAL 740
Lambda K H
0.315 0.131 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1529
Number of extensions: 53
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 741
Length of database: 741
Length adjustment: 40
Effective length of query: 701
Effective length of database: 701
Effective search space: 491401
Effective search space used: 491401
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 55 (25.8 bits)
Align candidate PfGW456L13_2641 (Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42))
to HMM TIGR00178 (isocitrate dehydrogenase, NADP-dependent (EC 1.1.1.42))
# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: ../tmp/path.carbon/TIGR00178.hmm
# target sequence database: /tmp/gapView.2488.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: TIGR00178 [M=744]
Accession: TIGR00178
Description: monomer_idh: isocitrate dehydrogenase, NADP-dependent
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
0 1370.3 8.4 0 1370.2 8.4 1.0 1 lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 Isocitrate dehydrogenase [NADP]
Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isoc
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 1370.2 8.4 0 0 5 742 .. 4 740 .. 1 741 [] 0.99
Alignments for each domain:
== domain 1 score: 1370.2 bits; conditional E-value: 0
TIGR00178 5 kakiiytltdeapllatysllpivkafaasaGievetrdislagrilaefpeylt 59
++kiiyt tdeap+latysllpiv+af+asa i+vetrdislagrila+fpe+l
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 4 RSKIIYTFTDEAPALATYSLLPIVEAFTASADIAVETRDISLAGRILASFPEQLG 58
59****************************************************8 PP
TIGR00178 60 eeqkvddalaelGelaktpeaniiklpnisasvpqlkaaikelqdkGydlpdype 114
+ + v d+laelG la tpeaniiklpnisasvpql+aaikelq++Gy+lpdype
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 59 D-KAVADHLAELGALAVTPEANIIKLPNISASVPQLQAAIKELQAQGYALPDYPE 112
6.68*************************************************** PP
TIGR00178 115 epktdeekdikaryakikGsavnpvlreGnsdrraplavkeyarkhphkmGewsa 169
+ d++k+ kary+kikGsavnpvlreGnsdrrapl+vk+yarkhphkmG+w++
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 113 TVTCDADKEAKARYDKIKGSAVNPVLREGNSDRRAPLSVKNYARKHPHKMGAWAK 167
******************************************************* PP
TIGR00178 170 dskshvahmdagdfyaseksvlldaaeevkieliakdGketvlkaklklldgevi 224
dsksh+ahm++gdfy+sek++l+daa+ vkielia+dG++tvlk+k+++++ge++
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 168 DSKSHIAHMSTGDFYGSEKAALIDAADAVKIELIAQDGTATVLKEKTSVQAGEIL 222
******************************************************* PP
TIGR00178 225 dssvlskkalvefleeeiedakeegvllslhlkatmmkvsdpivfGhvvrvfykd 279
d++vlsk+al+ f+++eiedak++gvlls+hlkatmmkvsdpi+fG++v +fykd
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 223 DCAVLSKNALRSFIAAEIEDAKAQGVLLSVHLKATMMKVSDPIMFGQIVAEFYKD 277
******************************************************* PP
TIGR00178 280 vfakhaelleqlGldvenGladlyakieslpaakkeeieadlekvyeerpelamv 334
++akha++l+q+G++ +nG++dlya+i++lpa ++ +iead+++vy+ rp lamv
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 278 ALAKHADVLSQIGFNLNNGIGDLYARIKALPAEQQAQIEADIQAVYAVRPSLAMV 332
******************************************************* PP
TIGR00178 335 dsdkGitnlhvpsdvivdasmpamirasGkmygkdgklkdtkavipdssyagvyq 389
+sdkGitnlhvpsdvivdasmpamir+sGkm+g+dg+l+dtkavipd++ya +yq
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 333 NSDKGITNLHVPSDVIVDASMPAMIRDSGKMWGTDGQLHDTKAVIPDRCYATIYQ 387
******************************************************* PP
TIGR00178 390 aviedckknGafdpttmGtvpnvGlmaqkaeeyGshdktfeieadGvvrvvdssG 444
aviedck nGafdpttmG+vpnvGlma+kaeeyGshdktf+i+adGvvrv+ds+G
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 388 AVIEDCKANGAFDPTTMGSVPNVGLMAKKAEEYGSHDKTFQIKADGVVRVTDSKG 442
******************************************************* PP
TIGR00178 445 evlleeeveagdiwrmcqvkdapiqdwvklavtrarlsgtpavfwldperahdee 499
++l+e+ veagdi+rmcq kdapiqdwvklav+rar+s+tpa+fwldp rahd
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 443 NLLMEQAVEAGDIFRMCQTKDAPIQDWVKLAVNRARASSTPAIFWLDPMRAHDGV 497
******************************************************* PP
TIGR00178 500 likkvekylkdhdteGldiqilspvkatrfslerirrGedtisvtGnvlrdyltd 554
+i+kv+ ylkdhdt Gldiqi+ pv+a++ +l+r r G+dtisvtGnvlrdyltd
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 498 VIEKVQAYLKDHDTAGLDIQIMAPVDAMKSTLQRTRAGKDTISVTGNVLRDYLTD 552
******************************************************* PP
TIGR00178 555 lfpilelGtsakmlsvvplmaGGGlfetGaGGsapkhvqqleeenhlrwdslGef 609
lfpi+elGtsakmls+vplm+GGGlfetGaGGsapkhvqql een+lrwdslGef
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 553 LFPIMELGTSAKMLSIVPLMNGGGLFETGAGGSAPKHVQQLLEENFLRWDSLGEF 607
******************************************************* PP
TIGR00178 610 lalaaslehvavktgnekakvladtldaatgklldeekspsrkvGeldnrgskfy 664
lalaasleh++v+++n+ka vla+tld+atg++ld++kspsrkvG++dnrgs+fy
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 608 LALAASLEHLGVTYNNPKALVLAKTLDQATGQFLDNNKSPSRKVGNIDNRGSHFY 662
******************************************************* PP
TIGR00178 665 lakywaqelaaqtedkelaasfasvaealtkneekivaelaavqGeavdlgGyya 719
la ywaq+laaqted+ l+a+fa++a++lt+ne++ivael+avqG++vd+gGyy+
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 663 LALYWAQALAAQTEDAALQAQFATLAKTLTENEATIVAELNAVQGKPVDIGGYYH 717
******************************************************* PP
TIGR00178 720 pdtdlttkvlrpsatfnaileal 742
+d +l++k++rpsatfna+++al
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2641 718 ADAELISKAMRPSATFNAAVAAL 740
********************998 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (744 nodes)
Target sequences: 1 (741 residues searched)
Passed MSV filter: 1 (1); expected 0.0 (0.02)
Passed bias filter: 1 (1); expected 0.0 (0.02)
Passed Vit filter: 1 (1); expected 0.0 (0.001)
Passed Fwd filter: 1 (1); expected 0.0 (1e-05)
Initial search space (Z): 1 [actual number of targets]
Domain search space (domZ): 1 [number of targets reported over threshold]
# CPU time: 0.04u 0.01s 00:00:00.05 Elapsed: 00:00:00.04
# Mc/sec: 11.26
//
[ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory