Alignments for a candidate for gabT in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for gabT in Pseudomonas fluorescens GW456-L13

Align 4-aminobutyrate transaminase subunit (EC 2.6.1.19) (characterized)
to candidate PfGW456L13_494 5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)

Query= metacyc::MONOMER-11537
         (425 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494
          Length = 425

 Score =  819 bits (2116), Expect = 0.0
 Identities = 414/425 (97%), Positives = 420/425 (98%)

Query: 1   MSKTNASLMKRREAAVPRGVGQIHPIFADSAKNATVTDVEGREFIDFAGGIAVLNTGHVH 60
           MSKTNASLM RR AAVPRGVGQIHPIFADSAKNATVTDVEGREFIDFAGGIAVLNTGHVH
Sbjct: 1   MSKTNASLMARRTAAVPRGVGQIHPIFADSAKNATVTDVEGREFIDFAGGIAVLNTGHVH 60

Query: 61  PKIIAAVTEQLNKLTHTCFQVLAYEPYVELCEKINAKVPGDFAKKTLLVTTGSEAVENAV 120
           PK+IAAVTEQLNKLTHTCFQVLAYEPYVE+CEKINAKVPGDFAKKTLLVTTGSEAVEN++
Sbjct: 61  PKVIAAVTEQLNKLTHTCFQVLAYEPYVEVCEKINAKVPGDFAKKTLLVTTGSEAVENSI 120

Query: 121 KIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGGIFRALYPNELHGVS 180
           KIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGGIFRALYPNELHGVS
Sbjct: 121 KIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGGIFRALYPNELHGVS 180

Query: 181 IDDSIASIERIFKNDAEPRDIAAIIIEPVQGEGGFYVAPKEFMKRLRALCDQHGILLIAD 240
           IDDSIASIERIFKNDAEP+DIAAIIIEPVQGEGGFYVAPKEFMKRLRALCDQHGILLIAD
Sbjct: 181 IDDSIASIERIFKNDAEPKDIAAIIIEPVQGEGGFYVAPKEFMKRLRALCDQHGILLIAD 240

Query: 241 EVQTGAGRTGTFFAMEQMGVTADLTTFAKSIAGGFPLAGVCGKAEYMDAIAPGGLGGTYA 300
           EVQTGAGRTGTFFAMEQMGV ADLTTFAKSIAGGFPLAGVCGKAEYMDAIAPGGLGGTYA
Sbjct: 241 EVQTGAGRTGTFFAMEQMGVAADLTTFAKSIAGGFPLAGVCGKAEYMDAIAPGGLGGTYA 300

Query: 301 GSPIACAAALAVMEVFEEEHLLDRCKAVGERLVTGLKAIQAKYPVIGEVRALGAMIAVEL 360
           GSPIACAAALAVMEVFEEEHLLDRCKAVGERLVTGLKAIQAKYPVIGEVRALGAMIAVEL
Sbjct: 301 GSPIACAAALAVMEVFEEEHLLDRCKAVGERLVTGLKAIQAKYPVIGEVRALGAMIAVEL 360

Query: 361 FENGDSHKPNAAAVAQVVAKARDKGLILLSCGTYGNVLRVLVPLTSPDAQLDKGLAIIEE 420
           FENGDSHKPNAAAVA VVAKARDKGLILLSCGTYGNVLRVLVPLTSPD QLDKGLAIIEE
Sbjct: 361 FENGDSHKPNAAAVASVVAKARDKGLILLSCGTYGNVLRVLVPLTSPDEQLDKGLAIIEE 420

Query: 421 CFAEL 425
           CF+EL
Sbjct: 421 CFSEL 425


Lambda     K      H
   0.320    0.137    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 822
Number of extensions: 22
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 425
Length of database: 425
Length adjustment: 32
Effective length of query: 393
Effective length of database: 393
Effective search space:   154449
Effective search space used:   154449
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

Align candidate PfGW456L13_494 (5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19))
to HMM TIGR00700 (gabT: 4-aminobutyrate transaminase (EC 2.6.1.19))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00700.hmm
# target sequence database:        /tmp/gapView.30316.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00700  [M=420]
Accession:   TIGR00700
Description: GABAtrnsam: 4-aminobutyrate transaminase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                              Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                              -----------
   1.7e-165  536.6   7.4   1.9e-165  536.4   7.4    1.0  1  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494  5-aminovalerate aminotransferase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494  5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobu
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  536.4   7.4  1.9e-165  1.9e-165       2     418 ..      11     422 ..      10     424 .. 0.99

  Alignments for each domain:
  == domain 1  score: 536.4 bits;  conditional E-value: 1.9e-165
                                              TIGR00700   2 rraaavskGvgvtlrvlaakaegaelkdvdGnrlidlaagiavlnvGhshPkvvea 57 
                                                            rr+aav++Gvg   +++a +a++a++ dv+G+++id+a+giavln+Gh hPkv++a
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494  11 RRTAAVPRGVGQIHPIFADSAKNATVTDVEGREFIDFAGGIAVLNTGHVHPKVIAA 66 
                                                            89****************************************************** PP

                                              TIGR00700  58 vkrqveelthtafqvvpyesyvelaeklnaiaPgsgekkavllnsGaeavenavki 113
                                                            v +q+++ltht+fqv+ ye yve+ ek+na  Pg   kk++l+++G+eaven++ki
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494  67 VTEQLNKLTHTCFQVLAYEPYVEVCEKINAKVPGDFAKKTLLVTTGSEAVENSIKI 122
                                                            ******************************************************** PP

                                              TIGR00700 114 arkytgrpgvvafsrgfhGrtnltmaltakvkPykiGfGPfapevyraPlpydyrd 169
                                                            ar+ tgr gv+af++++hGrt +t+ lt+kv Py  G+G +++ ++ra +p ++++
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494 123 ARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGGIFRALYPNELHG 178
                                                            *******************************************************9 PP

                                              TIGR00700 170 ialdkqeslddelaaiealfvadveaeqvaavvlePvqGeGGfivpakelvaavas 225
                                                             +       dd +a+ie++f+ d e++++aa+++ePvqGeGGf v++ke+++ ++ 
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494 179 VSI------DDSIASIERIFKNDAEPKDIAAIIIEPVQGEGGFYVAPKEFMKRLRA 228
                                                            877......88899****************************************** PP

                                              TIGR00700 226 lckehgivliadevqtGfartGklfaieheddkPdlitvaksladGlPlsgvvGra 281
                                                            lc++hgi+liadevqtG  rtG++fa+e +++  dl t aks+a+G+Pl+gv G+a
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494 229 LCDQHGILLIADEVQTGAGRTGTFFAMEQMGVAADLTTFAKSIAGGFPLAGVCGKA 284
                                                            ******************************************************** PP

                                              TIGR00700 282 eildapapGglGGtyaGnPlavaaalavldiieeeglieraeqigklvkdklielk 337
                                                            e +da apGglGGtyaG+P+a+aaalav+++ eee l +r++ +g+++   l  ++
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494 285 EYMDAIAPGGLGGTYAGSPIACAAALAVMEVFEEEHLLDRCKAVGERLVTGLKAIQ 340
                                                            ******************************************************** PP

                                              TIGR00700 338 eevpaigdvrglGamiavelv.dpdttePdaalaekiaaaalaaGlllltaGifGn 392
                                                            ++ p+ig+vr lGamiavel  + d+++P+aa  + + a+a+ +Gl+ll++G +Gn
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494 341 AKYPVIGEVRALGAMIAVELFeNGDSHKPNAAAVASVVAKARDKGLILLSCGTYGN 396
                                                            *******************9968899****************************** PP

                                              TIGR00700 393 iirlltPltisdelldeglkileaal 418
                                                            ++r+l Plt +de+ld+gl i+e+ +
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_494 397 VLRVLVPLTSPDEQLDKGLAIIEECF 422
                                                            **********************9876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (420 nodes)
Target sequences:                          1  (425 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 10.32
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources