Align 1-phosphofructokinase (EC 2.7.1.56) (characterized)
to candidate PfGW456L13_5074 1-phosphofructokinase (EC 2.7.1.56)
Query= reanno::pseudo5_N2C3_1:AO356_07330 (313 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5074 Length = 312 Score = 523 bits (1346), Expect = e-153 Identities = 271/313 (86%), Positives = 285/313 (91%), Gaps = 1/313 (0%) Query: 1 MAKILTLTLNPALDLTVELARLEPGQVNRSDAMHAHAAGKGVNVAQVLADLGHTLTVSGF 60 MA+ILTLTLNPALDLTV+L RL+ GQVNRSD MH HAAGKGVNVAQVLADLGH LTVSGF Sbjct: 1 MARILTLTLNPALDLTVQLPRLDAGQVNRSDVMHTHAAGKGVNVAQVLADLGHQLTVSGF 60 Query: 61 LGEDNAQVFETLFAQRGFVDAFIRVPGETRSNIKLAEQDGRITDLNGPGPMVDAAAQQAL 120 LGEDN Q FETLFA+RGFVDAFIRVPGETRSNIKLAE DGRITD+NGPGP V AAQQAL Sbjct: 61 LGEDNLQAFETLFAKRGFVDAFIRVPGETRSNIKLAESDGRITDINGPGPQVSEAAQQAL 120 Query: 121 LARLEQIAPGHDVVVVAGSLPRGVSPQWLQALIARMKALGLNVALDTSGEALRVALAAGP 180 + RLEQIAPGHD VVVAGSLP GVS QWLQALI R+K LGLNVALDTSGEALR AL AGP Sbjct: 121 MDRLEQIAPGHDAVVVAGSLPLGVSAQWLQALILRLKNLGLNVALDTSGEALRAALKAGP 180 Query: 181 WLIKPNTEELADALGCEVVSETAQAQAAQRLHAQGIEHVVISHGADGVNWFSVGAALHAS 240 WLIKPNTEELA+ LG EVVS +AQA AA+RLHAQGIEHVVISHG DGVNWFSVG+ALHA+ Sbjct: 181 WLIKPNTEELAEVLG-EVVSVSAQALAAERLHAQGIEHVVISHGVDGVNWFSVGSALHAT 239 Query: 241 PPKVSVASTVGAGDSLLAGMLHGLLSADTPEQTLRTATAIAAMAVTQIGFGIHDTALLAS 300 PP+V+VASTVGAGDSLLAGMLHGLLSADTPEQTLRTATAIAAMAVTQIGFGI D A LA Sbjct: 240 PPRVNVASTVGAGDSLLAGMLHGLLSADTPEQTLRTATAIAAMAVTQIGFGIGDAAQLAQ 299 Query: 301 LEQGVRVRPLTEQ 313 LEQGVRVRPLTEQ Sbjct: 300 LEQGVRVRPLTEQ 312 Lambda K H 0.317 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 434 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 313 Length of database: 312 Length adjustment: 27 Effective length of query: 286 Effective length of database: 285 Effective search space: 81510 Effective search space used: 81510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
Align candidate PfGW456L13_5074 (1-phosphofructokinase (EC 2.7.1.56))
to HMM TIGR03828 (pfkB: 1-phosphofructokinase (EC 2.7.1.56))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03828.hmm # target sequence database: /tmp/gapView.15585.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03828 [M=305] Accession: TIGR03828 Description: pfkB: 1-phosphofructokinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.6e-106 341.7 1.7 1.8e-106 341.5 1.7 1.0 1 lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5074 1-phosphofructokinase (EC 2.7.1. Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5074 1-phosphofructokinase (EC 2.7.1.56) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 341.5 1.7 1.8e-106 1.8e-106 1 304 [. 4 306 .. 4 307 .. 0.98 Alignments for each domain: == domain 1 score: 341.5 bits; conditional E-value: 1.8e-106 TIGR03828 1 IlTvTlNpaiDktieleelelgevnrveserldagGKGinVarvLkklgvevval 55 IlT+TlNpa+D+t++l +l++g+vnr ++++a+GKG+nVa+vL++lg++ +++ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5074 4 ILTLTLNPALDLTVQLPRLDAGQVNRSDVMHTHAAGKGVNVAQVLADLGHQLTVS 58 89***************************************************** PP TIGR03828 56 gflGgftgeeiealleeegiktdfvevkgetRinvkikessgeetklnepGpeis 110 gflG+++ + +e+l++++g+ ++f++v+getR+n+k++es+g+ t++n+pGp++s lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5074 59 GFLGEDNLQAFETLFAKRGFVDAFIRVPGETRSNIKLAESDGRITDINGPGPQVS 113 ******************************************************* PP TIGR03828 111 eeeleallekleeqlkegdvlvlaGSlPrgvpedlyaelikllrekgakvilDts 165 e++++al+++le+ ++ +d +v+aGSlP gv++++++ li +l++ g++v+lDts lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5074 114 EAAQQALMDRLEQIAPGHDAVVVAGSLPLGVSAQWLQALILRLKNLGLNVALDTS 168 ************999**************************************** PP TIGR03828 166 geaLlkvlkakplliKPNkeEleellgrelkteeevieaarkllekgvenvlisl 220 geaL+++lka p+liKPN+eEl+e+lg+ ++ ++ aa++l+++g+e+v+is lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5074 169 GEALRAALKAGPWLIKPNTEELAEVLGEVVSV-SAQALAAERLHAQGIEHVVISH 222 ***************************99965.66777999************** PP TIGR03828 221 GadGallvtkegalfakapkievkstvGAGDsmvAgfllalekglsleealrlav 275 G dG+ +++ al+a++p+++v+stvGAGDs++Ag+l++l + ++e++lr+a+ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5074 223 GVDGVNWFSVGSALHATPPRVNVASTVGAGDSLLAGMLHGLLSADTPEQTLRTAT 277 ******************************************************* PP TIGR03828 276 Avgaaaassegtelpdledieelleevki 304 A++a a+++ g++++d +++++l++ v++ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5074 278 AIAAMAVTQIGFGIGDAAQLAQLEQGVRV 306 ***************************98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (305 nodes) Target sequences: 1 (312 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 10.06 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory