Alignments for a candidate for garD in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for garD in Pseudomonas fluorescens GW456-L13

Align Galactarate dehydratase (EC 4.2.1.42) (characterized)
to candidate PfGW456L13_5042 D-galactarate dehydratase (EC 4.2.1.42)

Query= reanno::WCS417:GFF829
         (517 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042
          Length = 517

 Score = 1002 bits (2590), Expect = 0.0
 Identities = 497/517 (96%), Positives = 507/517 (98%)

Query: 1   MQLIEHADSPRSIRLHERDNVVIVVNDQGVPAGTEFPDGLVTVDFIPQSHKVTLEDIPEG 60
           MQLIEH+DSPR IRLHERDNVVIVVNDQGVPAGTEF DGLVTVDF+PQSHKVTLEDIPEG
Sbjct: 1   MQLIEHSDSPRYIRLHERDNVVIVVNDQGVPAGTEFADGLVTVDFVPQSHKVTLEDIPEG 60

Query: 61  GQIIRYGQTIGYALAPIPRGSWVQEDQLRMPTAPPLDSLPLSTEVPEAQAPLEGFTFEGY 120
           GQ+IRYGQ IGYAL PIPRGSWV+EDQLRMPTAPPLDSLPLST+VP AQAPLEGFTFEGY
Sbjct: 61  GQVIRYGQVIGYALQPIPRGSWVKEDQLRMPTAPPLDSLPLSTDVPAAQAPLEGFTFEGY 120

Query: 121 RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPKYPHVDDVVALTHSYGCGVAI 180
           RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPKYPHVDDVVALTHSYGCGVAI
Sbjct: 121 RNADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPKYPHVDDVVALTHSYGCGVAI 180

Query: 181 TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHDNDSSVDLSEPWLYRLQD 240
           TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMH+NDSSVDLSEPWLYRLQD
Sbjct: 181 TATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHENDSSVDLSEPWLYRLQD 240

Query: 241 SSHGFTEMIEQIMALAETRLKKLDQRRRETVPASELILGMQCGGSDAFSGITANPALGYA 300
           SSHGFTEMIEQIM LAETRLKKLDQRRRETVPASELILGMQCGGSDAFSGITANPALGYA
Sbjct: 241 SSHGFTEMIEQIMELAETRLKKLDQRRRETVPASELILGMQCGGSDAFSGITANPALGYA 300

Query: 301 SDLLLRAGATVMFSEVTEVRDAIYLLTSRAENTQVAQELVREMDWYDRYLAKGEADRSAN 360
           SDLLLRAGATVMFSEVTEVRDAIYLLTSRAEN  VAQELVREMDWYDRYLAKGEADRSAN
Sbjct: 301 SDLLLRAGATVMFSEVTEVRDAIYLLTSRAENQDVAQELVREMDWYDRYLAKGEADRSAN 360

Query: 361 TTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERFKGKGLIFCATPASDFVCGTLQ 420
           TTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERFK KGLIFCATPASDFVCGTLQ
Sbjct: 361 TTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPGERFKRKGLIFCATPASDFVCGTLQ 420

Query: 421 LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRATIEELG 480
           LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRA+IE+LG
Sbjct: 421 LAAGMNLHVFTTGRGTPYGLAMAPVVKVSTRTELAQRWPDLIDIDAGRIATGRASIEDLG 480

Query: 481 WELFHFYLDVASGRKQTWAEHHKLHNDITLFNPAPIT 517
           WELFH+YLDVASG+KQTWAE HKLHNDITLFNPAPIT
Sbjct: 481 WELFHYYLDVASGKKQTWAEQHKLHNDITLFNPAPIT 517


Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 985
Number of extensions: 17
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 517
Length of database: 517
Length adjustment: 35
Effective length of query: 482
Effective length of database: 482
Effective search space:   232324
Effective search space used:   232324
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

Align candidate PfGW456L13_5042 (D-galactarate dehydratase (EC 4.2.1.42))
to HMM TIGR03248 (garD: galactarate dehydratase (EC 4.2.1.42))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03248.hmm
# target sequence database:        /tmp/gapView.8110.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03248  [M=507]
Accession:   TIGR03248
Description: galactar-dH20: galactarate dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                               -----------
   5.7e-295  964.4   0.0   6.4e-295  964.2   0.0    1.0  1  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042  D-galactarate dehydratase (EC 4.


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042  D-galactarate dehydratase (EC 4.2.1.42)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  964.2   0.0  6.4e-295  6.4e-295       2     507 .]      12     517 .]      11     517 .] 1.00

  Alignments for each domain:
  == domain 1  score: 964.2 bits;  conditional E-value: 6.4e-295
                                               TIGR03248   2 yirvneqdnvaivvndkGlpagtefedgltlvekipqghkvalvdlekgdaiiry 56 
                                                             yir++e+dnv+ivvnd+G+pagtef+dgl++v+ +pq+hkv+l d+ +g ++iry
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042  12 YIRLHERDNVVIVVNDQGVPAGTEFADGLVTVDFVPQSHKVTLEDIPEGGQVIRY 66 
                                                             9****************************************************** PP

                                               TIGR03248  57 geviGyavkdiarGswvkeellelpsapaleelplatkvpeklapleGytfeGyr 111
                                                             g+viGya++ i+rGswvke++l++p+ap+l++lpl+t vp+++apleG+tfeGyr
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042  67 GQVIGYALQPIPRGSWVKEDQLRMPTAPPLDSLPLSTDVPAAQAPLEGFTFEGYR 121
                                                             ******************************************************* PP

                                               TIGR03248 112 nadGsvGtknilgittsvqcvagvvdyavkrikkellpkypnvddvvalnhsyGc 166
                                                             nadG+vGt+nilgitt+vqcv+gv+d+avkrik+ellpkyp vddvval+hsyGc
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042 122 NADGTVGTRNILGITTTVQCVTGVLDHAVKRIKDELLPKYPHVDDVVALTHSYGC 176
                                                             ******************************************************* PP

                                               TIGR03248 167 GvaidapdaivpirtlrnlalnpnlGGealvvglGceklqperllpeelsavelk 221
                                                             Gvai+a+da++pirt+rnla+npnlGGealv++lGceklq+ ++++e+ s+v+l+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042 177 GVAITATDAYIPIRTVRNLARNPNLGGEALVISLGCEKLQAGQVMHENDSSVDLS 231
                                                             ******************************************************* PP

                                               TIGR03248 222 daavlrlqdeklGfaemveailelaeerlkklnarkretvpaselvvGlqcGGsd 276
                                                             +++++rlqd+++Gf+em+e+i+elae+rlkkl++r+retvpasel++G+qcGGsd
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042 232 EPWLYRLQDSSHGFTEMIEQIMELAETRLKKLDQRRRETVPASELILGMQCGGSD 286
                                                             ******************************************************* PP

                                               TIGR03248 277 afsGvtanpavGfaadllvraGatvlfsevtevrdaihlltpraedaevakalir 331
                                                             afsG+tanpa+G+a+dll+raGatv+fsevtevrdai+llt+rae+++va+ l+r
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042 287 AFSGITANPALGYASDLLLRAGATVMFSEVTEVRDAIYLLTSRAENQDVAQELVR 341
                                                             ******************************************************* PP

                                               TIGR03248 332 emkwydeylarGeadrsanttpGnkkGGlsnivekalGsivksGssaivevlspG 386
                                                             em+wyd+yla+GeadrsanttpGnkkGGlsnivek+lGsivksGssai++vl+pG
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042 342 EMDWYDRYLAKGEADRSANTTPGNKKGGLSNIVEKSLGSIVKSGSSAINGVLGPG 396
                                                             ******************************************************* PP

                                               TIGR03248 387 ekvkkkGliyaatpasdfvcGtlqlasglnlhvfttGrGtpyGlalvpvikvstr 441
                                                             e+ k+kGli++atpasdfvcGtlqla+g+nlhvfttGrGtpyGla++pv+kvstr
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042 397 ERFKRKGLIFCATPASDFVCGTLQLAAGMNLHVFTTGRGTPYGLAMAPVVKVSTR 451
                                                             ******************************************************* PP

                                               TIGR03248 442 telaerwadlidldaGriatGeatiedvGwelfrlildvasGrkktwaekyklhn 496
                                                             tela+rw dlid+daGriatG+a+ied+Gwelf+ +ldvasG+k+twae++klhn
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042 452 TELAQRWPDLIDIDAGRIATGRASIEDLGWELFHYYLDVASGKKQTWAEQHKLHN 506
                                                             ******************************************************* PP

                                               TIGR03248 497 dlalfnpapvt 507
                                                             d++lfnpap+t
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5042 507 DITLFNPAPIT 517
                                                             **********8 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (507 nodes)
Target sequences:                          1  (517 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 9.96
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources