Alignments for a candidate for kdgD in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for kdgD in Pseudomonas fluorescens GW456-L13

Align 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41) (characterized)
to candidate PfGW456L13_5045 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41)

Query= reanno::pseudo1_N1B4:Pf1N1B4_1110
         (303 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5045
          Length = 303

 Score =  591 bits (1524), Expect = e-174
 Identities = 299/303 (98%), Positives = 302/303 (99%)

Query: 1   MNPQELKSILSSGLLSFPVTDFNAQGDFHRAGYIKRLEWLAPYGASALFAAGGTGEFFSL 60
           MNPQELKSILSSGLLSFPVTDFNAQGDF+RAGYIKRLEWLAPYGASALFAAGGTGEFFSL
Sbjct: 1   MNPQELKSILSSGLLSFPVTDFNAQGDFNRAGYIKRLEWLAPYGASALFAAGGTGEFFSL 60

Query: 61  AASEYSEIIKTAVDTCATSVPILAGVGGATRQAIEYAQEAERLGAKGLLLLPHYLTEASQ 120
           AASEYS+IIKTAVDTCATSVPILAGVGG+TRQAIEYAQEAERLGAKGLLLLPHYLTEASQ
Sbjct: 61  AASEYSQIIKTAVDTCATSVPILAGVGGSTRQAIEYAQEAERLGAKGLLLLPHYLTEASQ 120

Query: 121 DGVAAHVEAVCKSVKIGVVVYNRNVCRLTAPLLERLAERCPNLIGYKDGLGDIELMVSIR 180
           DGVAAHVEAVCKSV IGVVVYNRNVCRLTAPLLERLAERCPNLIGYKDGLGDIELMVSIR
Sbjct: 121 DGVAAHVEAVCKSVNIGVVVYNRNVCRLTAPLLERLAERCPNLIGYKDGLGDIELMVSIR 180

Query: 181 RRLGDRFSYLGGLPTAEVYAAAYKALGVPVYSSAVFNFIPKTAMDFYHAIAREDHATVGK 240
           RRLGDRFSYLGGLPTAEVYAAAYKALGVPVYSSAVFNFIPKTAMDFYHAIAREDHATVGK
Sbjct: 181 RRLGDRFSYLGGLPTAEVYAAAYKALGVPVYSSAVFNFIPKTAMDFYHAIAREDHATVGK 240

Query: 241 IIDDFFLPYLDIRNRKAGYAVSIVKAGAKIAGYDAGPVRAPLTDLTGEEYEMLAALIDKQ 300
           IIDDFFLPYLDIRNRKAGYAVSIVKAGAKIAGYDAGPVRAPLTDLTGEEYEMLAALIDKQ
Sbjct: 241 IIDDFFLPYLDIRNRKAGYAVSIVKAGAKIAGYDAGPVRAPLTDLTGEEYEMLAALIDKQ 300

Query: 301 GAQ 303
           GAQ
Sbjct: 301 GAQ 303


Lambda     K      H
   0.320    0.137    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 486
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 303
Length of database: 303
Length adjustment: 27
Effective length of query: 276
Effective length of database: 276
Effective search space:    76176
Effective search space used:    76176
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

Align candidate PfGW456L13_5045 (5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41))
to HMM TIGR03249 (kdgD: 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03249.hmm
# target sequence database:        /tmp/gapView.26568.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03249  [M=299]
Accession:   TIGR03249
Description: KdgD: 5-dehydro-4-deoxyglucarate dehydratase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                               -----------
   4.2e-137  442.1   0.0   4.7e-137  442.0   0.0    1.0  1  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5045  5-dehydro-4-deoxyglucarate dehyd


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5045  5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  442.0   0.0  4.7e-137  4.7e-137       1     298 [.       3     300 ..       3     301 .. 0.99

  Alignments for each domain:
  == domain 1  score: 442.0 bits;  conditional E-value: 4.7e-137
                                               TIGR03249   1 pqelkkkigsGllsfPvtpfdadgsldeaalrenieflldedlealfvagGtGef 55 
                                                             pqelk  ++sGllsfPvt+f+a+g+++ a + +++e+l++++++alf+agGtGef
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5045   3 PQELKSILSSGLLSFPVTDFNAQGDFNRAGYIKRLEWLAPYGASALFAAGGTGEF 57 
                                                             89***************************************************** PP

                                               TIGR03249  56 fsltkaeveqvvevaveaakgkvPvlagvGgnlsvaieiaraaekkGadGllllP 110
                                                             fsl  +e++q+++ av++++  vP+lagvGg++++aie+a++ae+ Ga+GllllP
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5045  58 FSLAASEYSQIIKTAVDTCATSVPILAGVGGSTRQAIEYAQEAERLGAKGLLLLP 112
                                                             ******************************************************* PP

                                               TIGR03249 111 pylieaeqeGlaayvkaviesvdlgvivyqrdnavleadtlerlaerlpnlvGfk 165
                                                             +yl+ea+q+G+aa+v+av++sv++gv+vy+r+ + l+a+ lerlaer+pnl+G+k
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5045 113 HYLTEASQDGVAAHVEAVCKSVNIGVVVYNRNVCRLTAPLLERLAERCPNLIGYK 167
                                                             ******************************************************* PP

                                               TIGR03249 166 dGiGdiekvieitqklGdrllylgGlPtaevtalaylalGvtsyssaifnfiPki 220
                                                             dG+Gdie +++i+++lGdr+ ylgGlPtaev+a+ay+alGv +yssa+fnfiPk+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5045 168 DGLGDIELMVSIRRRLGDRFSYLGGLPTAEVYAAAYKALGVPVYSSAVFNFIPKT 222
                                                             ******************************************************* PP

                                               TIGR03249 221 arkfyealrkgdeatvkeilkevilPiveirnrkkGyavslikaGlevvGrdvgp 275
                                                             a++fy+a++++d+atv +i+++++lP++ irnrk+Gyavs++kaG+++ G d+gp
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5045 223 AMDFYHAIAREDHATVGKIIDDFFLPYLDIRNRKAGYAVSIVKAGAKIAGYDAGP 277
                                                             ******************************************************* PP

                                               TIGR03249 276 vraPlvdlekeelaeleellkka 298
                                                             vraPl+dl+ ee++ l++l++k+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5045 278 VRAPLTDLTGEEYEMLAALIDKQ 300
                                                             *******************9985 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (299 nodes)
Target sequences:                          1  (303 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 8.73
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources