Align ABC transporter for L-Histidine, permease component 1 (characterized)
to candidate PfGW456L13_3607 Amino acid ABC transporter, permease protein
Query= reanno::acidovorax_3H11:Ac3H11_2554 (222 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3607 Length = 281 Score = 135 bits (340), Expect = 8e-37 Identities = 68/202 (33%), Positives = 122/202 (60%), Gaps = 1/202 (0%) Query: 11 GVPQLLAGALVTVEITAASLLLGCVMGLLVGIGRLNPKRRVVYALCTAYVAAIRGTPLLV 70 G L GA +T+ + S+ L ++G + + RL+ + V + + + Y + RGTPLL+ Sbjct: 70 GPDGFLQGAALTLFLCFCSIWLSLLLGFVTALARLS-RSAVAFGVASFYASFFRGTPLLI 128 Query: 71 QLFILFFGLPQFGILLPAFVCGVIGLGIYSGAYVSEVVRGAIQSIDKGQMEAARSIGMSS 130 Q+ +++ GLPQ G++ A G+I L + GAY+SE+ R I ++ GQ EAA ++G+ Sbjct: 129 QILLIYLGLPQLGVVPGAISAGIIALSLNYGAYLSEIFRAGIMAVAPGQREAAMALGLGP 188 Query: 131 GLAMRTVVLPQAVVRMIPPLGNEFIALIKNSALVSLLTIHDLMHEGQKIISVSYRSLEVY 190 +VLPQA+ +IPP ++FI+++K+S+L+S++ + ++M Q SYR +E+ Sbjct: 189 VATFLHIVLPQAMRTIIPPTTSQFISMLKDSSLISVMGVWEVMFLAQSYGRSSYRYIEML 248 Query: 191 LAIAVVYFILTGATTLVLRRIE 212 AV+Y++L+ L+ R+E Sbjct: 249 TTAAVIYWLLSIGLELIQNRLE 270 Lambda K H 0.328 0.143 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 156 Number of extensions: 5 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 222 Length of database: 281 Length adjustment: 24 Effective length of query: 198 Effective length of database: 257 Effective search space: 50886 Effective search space used: 50886 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory