GapMind for catabolism of small carbon sources

 

Alignments for a candidate for BPHYT_RS24005 in Pseudomonas fluorescens GW456-L13

Align Polar amino acid ABC transporter, inner membrane subunit; Flags: Precursor (characterized, see rationale)
to candidate PfGW456L13_384 Histidine ABC transporter, permease protein HisQ (TC 3.A.1.3.1)

Query= uniprot:B2TBJ7
         (240 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_384
          Length = 242

 Score =  178 bits (451), Expect = 1e-49
 Identities = 90/226 (39%), Positives = 145/226 (64%), Gaps = 1/226 (0%)

Query: 1   MALIQMLGFGPEGWGGVLLLAALMTVALTLAALAVGAVFGALVAAAKLSRFRTLRVIGDI 60
           + ++ + GF  +G+G +LL  + MTV L+   L V    G + A+AKLS+   LRV    
Sbjct: 6   LMILGLSGFSLKGFGPLLLQGSWMTVKLSFLCLLVSVGLGLIGASAKLSKSALLRVPAQA 65

Query: 61  YTTVFRGVPELLVIYLFYFGGSTLVTSVGQLFGAEGFVGVPPFVVGALAVGMISGAYQAE 120
           YTT+ RGVP+L+++ L ++   T +TS+ +  G + ++ + PF  G + +G I GAY  E
Sbjct: 66  YTTLIRGVPDLVLMLLIFYSLQTWLTSLTEALGWD-YIEINPFSAGVITLGFIYGAYFTE 124

Query: 121 VYRSAVLAVSRGELEAARSIGMPTLTMARRILIPQVLRFALPGIGNVWQLSLKDSALISV 180
            +R A+LAV RG++EAA + G+      R ++ PQ++RFALPGIGN W + LK +AL+S+
Sbjct: 125 TFRGAILAVPRGQMEAATAYGLTRAQRFRLVVFPQMMRFALPGIGNNWMVILKATALVSI 184

Query: 181 TGLAELLRTSQVAAGSTHQYFTFFVVGGALYLIMTSISNRVFNRAE 226
            GLA+L++ +Q A  S++Q F F ++   +YL++T++SN V  R E
Sbjct: 185 IGLADLVKVAQDAGKSSYQLFFFLILAALIYLVITTVSNYVLRRME 230


Lambda     K      H
   0.327    0.141    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 125
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 240
Length of database: 242
Length adjustment: 23
Effective length of query: 217
Effective length of database: 219
Effective search space:    47523
Effective search space used:    47523
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory