Aligments for a candidate for fadA in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Aligments for a candidate for fadA in Pseudomonas fluorescens GW456-L13

Align subunit of β-ketoadipyl CoA thiolase (EC 2.3.1.174; EC 2.3.1.16) (characterized)
to candidate PfGW456L13_4590 Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)

Query= metacyc::MONOMER-3207
         (400 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590
          Length = 400

 Score =  713 bits (1841), Expect = 0.0
 Identities = 362/400 (90%), Positives = 384/400 (96%)

Query: 1   MRDVFICDAIRTPIGRFGGALAGVRADDLAAVPLKALIEPNPAVQWDQVDEVFFGCANQA 60
           MRDV+ICDAIRTPIGRFGG L+ VRADDLAAVP+KAL+E NP+V W+ VDEVF GCANQA
Sbjct: 1   MRDVYICDAIRTPIGRFGGGLSTVRADDLAAVPIKALMERNPSVDWNAVDEVFLGCANQA 60

Query: 61  GEDNRNVARMALLLAGLPESIPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESM 120
           GEDNRNVARMALLLAGLPE+IPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESM
Sbjct: 61  GEDNRNVARMALLLAGLPETIPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESM 120

Query: 121 SRAPFVMGKAESGYSRNMKLEDTTIGWRFINPLMKSQYGVDSMPETADNVADDYQVSRAD 180
           SRAPFVMGKA++ +SRNMKLEDTTIGWRFINPLMK+QYGVD+MP+TADNVADDY+VSRAD
Sbjct: 121 SRAPFVMGKADAAFSRNMKLEDTTIGWRFINPLMKAQYGVDAMPQTADNVADDYKVSRAD 180

Query: 181 QDAFALRSQQKAAAAQAAGFFAEEIVPVRIAHKKGETIVERDEHLRPETTLEALTKLKPV 240
           QDAFALRSQQ+ AAAQAAGFFAEEIV VRIAHKKGET+V +DEH R +TTLEALTKLKPV
Sbjct: 181 QDAFALRSQQRTAAAQAAGFFAEEIVEVRIAHKKGETVVSQDEHPRADTTLEALTKLKPV 240

Query: 241 NGPDKTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASGGVAPRVMGIGPVP 300
           NGPDKTVTAGNASGVNDGAAALILASAEAVKKHGLT RA+VLGMAS GVAPRVMGIGPVP
Sbjct: 241 NGPDKTVTAGNASGVNDGAAALILASAEAVKKHGLTARAKVLGMASAGVAPRVMGIGPVP 300

Query: 301 AVRKLTERLGVAVSDFDVIELNEAFASQGLAVLRELGVADDAPQVNPNGGAIALGHPLGM 360
           AVRKL ERLGVAVSDFDVIELNEAFASQGLAVLRELG+ADDA QVNPNGGAIALGHPLGM
Sbjct: 301 AVRKLIERLGVAVSDFDVIELNEAFASQGLAVLRELGIADDAAQVNPNGGAIALGHPLGM 360

Query: 361 SGARLVLTALHQLEKSGGRKGLATMCVGVGQGLALAIERV 400
           SGARLVLTALHQLEK+GG+KGLATMCVGVGQGLALAIERV
Sbjct: 361 SGARLVLTALHQLEKTGGKKGLATMCVGVGQGLALAIERV 400


Lambda     K      H
   0.318    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 648
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 400
Length of database: 400
Length adjustment: 31
Effective length of query: 369
Effective length of database: 369
Effective search space:   136161
Effective search space used:   136161
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate PfGW456L13_4590 (Beta-ketoadipyl CoA thiolase (EC 2.3.1.-))
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01930.hmm
# target sequence database:        /tmp/gapView.16819.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01930  [M=385]
Accession:   TIGR01930
Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                               -----------
   5.7e-137  442.7   6.9   6.4e-137  442.5   6.9    1.0  1  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590  Beta-ketoadipyl CoA thiolase (EC


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590  Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  442.5   6.9  6.4e-137  6.4e-137       1     385 []       6     398 ..       6     398 .. 0.95

  Alignments for each domain:
  == domain 1  score: 442.5 bits;  conditional E-value: 6.4e-137
                                               TIGR01930   1 ivdavRtpigklggslkelsaedLlaavikellera.gldpekidevilGnvlqa 54 
                                                             i da+Rtpig++gg l++++a+dL+a  ik+l+er+  +d +++dev lG++ qa
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590   6 ICDAIRTPIGRFGGGLSTVRADDLAAVPIKALMERNpSVDWNAVDEVFLGCANQA 60 
                                                             789**************************************************** PP

                                               TIGR01930  55 geq.aniaReaalaaglpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaG 108
                                                             ge+  n+aR+a+l aglpe++p++t+nr+CaSg++A+ +a ++i++Ge+++++aG
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590  61 GEDnRNVARMALLLAGLPETIPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAG 115
                                                             ***9*************************************************** PP

                                               TIGR01930 109 GvEsmSrvpillkaslrreslklgkakledqllkdl........vktklsmgetA 155
                                                             GvEsmSr+p+++ ++     +  +++kled+++           ++   +m +tA
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590 116 GVESMSRAPFVMGKAD---AAFSRNMKLEDTTIGWRfinplmkaQYGVDAMPQTA 167
                                                             **********999974...4444455555533332266777998777889***** PP

                                               TIGR01930 156 enlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk..kkvvskD 208
                                                             +n+a++y++sR +qD++alrS+q++a+A+++g+f++eiv v++ +k  ++vvs+D
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590 168 DNVADDYKVSRADQDAFALRSQQRTAAAQAAGFFAEEIVEVRIAHKkgETVVSQD 222
                                                             *******************************************9999999***** PP

                                               TIGR01930 209 egirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgl 263
                                                             e++r++ttle+L kLkp+  + +  tvtAgN+s++nDGAaal+l+s e++k++gl
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590 223 EHPRADTTLEALTKLKPVNGP-DK-TVTAGNASGVNDGAAALILASAEAVKKHGL 275
                                                             *******************95.77.7***************************** PP

                                               TIGR01930 264 tplarivsaavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvl 318
                                                             t  a++ ++a agv p++mg+gpvpA++k++++ g+ +sd+d++E+nEAFA+q l
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590 276 TARAKVLGMASAGVAPRVMGIGPVPAVRKLIERLGVAVSDFDVIELNEAFASQGL 330
                                                             ******************************************************* PP

                                               TIGR01930 319 avekelgsld.lekvNvnGGAiAlGHPlGasGarivltllkeLkergkkyGlatl 372
                                                             av +elg  d  ++vN nGGAiAlGHPlG+sGar+vlt l++L++ g+k Glat+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590 331 AVLRELGIADdAAQVNPNGGAIALGHPLGMSGARLVLTALHQLEKTGGKKGLATM 385
                                                             *******86679******************************************* PP

                                               TIGR01930 373 CvggGqGaAvile 385
                                                             Cvg GqG A+ +e
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4590 386 CVGVGQGLALAIE 398
                                                             *********9886 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (385 nodes)
Target sequences:                          1  (400 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.18
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources