GapMind for catabolism of small carbon sources

 

Alignments for a candidate for argT in Pseudomonas fluorescens GW456-L13

Align lysine/arginine/ornithine ABC transporter, periplasmic lysine/arginine/ornithine-binding protein ArgT (characterized)
to candidate PfGW456L13_1692 Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein

Query= CharProtDB::CH_003045
         (260 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1692
          Length = 258

 Score =  236 bits (601), Expect = 5e-67
 Identities = 116/260 (44%), Positives = 168/260 (64%), Gaps = 2/260 (0%)

Query: 1   MKKTVLALSLLIGLGATAASYAALPQTVRIGTDTTYAPFSSKDAKGEFIGFDIDLGNEMC 60
           MKK  L   L + L A ++ +AA  +T+RIG +  Y PF+SK  KGE +GFD D+GN +C
Sbjct: 1   MKKFPLITGLALSLLACSSVFAA-EKTLRIGIEAAYPPFASKTDKGEIVGFDYDIGNALC 59

Query: 61  KRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQEIAFSDKLYAADSRLIAAK 120
            +M+ KC WV  +FD LIPSLK KKID  +SS++I + R++ + F+ K Y   SRL+   
Sbjct: 60  AQMKAKCVWVEGEFDGLIPSLKVKKIDMALSSMTINEDRKKSVDFTHKYYFTSSRLVMKD 119

Query: 121 GSPIQPTLESLKGKHVGVLQGSTQEAYANDNWRTKGVDVVAYANQDLIYSDLTAGRLDAA 180
           G+ +     SLKGK VGV + +T + YA++ +  KG++V  Y+N + IY DL AGRLDA 
Sbjct: 120 GAVVDDQYASLKGKTVGVQRATTTDRYASEVFEPKGINVKRYSNNEEIYMDLAAGRLDAI 179

Query: 181 LQDEVAASEGFLKQPAGKEYAFAGPSVKDKKYFGDGTGVGLRKDDTELKAAFDKALTELR 240
             D +  ++ FL  P GK YAF GP +KD KY G+G G+ +RK +TEL +  + A+  +R
Sbjct: 180 FADTIPLND-FLSMPRGKGYAFVGPELKDPKYVGEGAGIAVRKGNTELVSELNTAIDGIR 238

Query: 241 QDGTYDKMAKKYFDFNVYGD 260
             G Y K+++KYF  ++YGD
Sbjct: 239 ASGEYQKISEKYFKSDIYGD 258


Lambda     K      H
   0.315    0.132    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 221
Number of extensions: 13
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 258
Length adjustment: 24
Effective length of query: 236
Effective length of database: 234
Effective search space:    55224
Effective search space used:    55224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory