GapMind for catabolism of small carbon sources

 

Aligments for a candidate for hisM in Pseudomonas fluorescens GW456-L13

Align ABC transporter for L-Lysine, permease component 2 (characterized)
to candidate PfGW456L13_3701 Histidine ABC transporter, permease protein HisM (TC 3.A.1.3.1)

Query= reanno::pseudo5_N2C3_1:AO356_09910
         (229 letters)



>lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3701 Histidine
           ABC transporter, permease protein HisM (TC 3.A.1.3.1)
          Length = 232

 Score =  220 bits (561), Expect = 2e-62
 Identities = 113/222 (50%), Positives = 160/222 (72%), Gaps = 1/222 (0%)

Query: 1   MNWDVIIKWLPKLAQGATLTLELVAIAVIAGLLLAIPLGIARSSRLWQVRALPYAYIFFF 60
           +++++I + LP    GA LTL+++ I++  GL+LAIPL + R SR   +    + Y +  
Sbjct: 3   LDYNLIWENLPLYFNGALLTLKVLLISLALGLVLAIPLALMRVSRSPLINFPAWLYTYVI 62

Query: 61  RGTPLLVQLFLVYYGLAQFDAVRSSALWPYLRDPFWCATVTMTLHTAAYIAEILRGAIQA 120
           RGTP+LVQLFL+YYGLAQF+AVR SALWPYL    +CA +   ++T+AY AE+L G++++
Sbjct: 63  RGTPMLVQLFLIYYGLAQFEAVRQSALWPYLSSATFCACLAFAINTSAYSAELLAGSLKS 122

Query: 121 IPKGEIEAARALGMSRPKALFYIMLPRAARIGLPAYSNEVILMLKASALASTVTLLELTG 180
            P GEIEAARA+GMSR      I++P A R  LP YSNEV++ML+ ++LAS VTL+++TG
Sbjct: 123 TPHGEIEAARAMGMSRLTLYRRILMPSALRRALPQYSNEVLMMLQTTSLASIVTLVDITG 182

Query: 181 MARTIIARTYLPVEIFFAAGMFYLLMSFLLVQGFKQLER-WL 221
            ART+ +R YLP E F  AG+FYL ++F+LV+ FK  ER WL
Sbjct: 183 AARTVSSRFYLPFEAFITAGLFYLALTFILVRLFKLAERHWL 224


Lambda     K      H
   0.331    0.141    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 181
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 229
Length of database: 232
Length adjustment: 23
Effective length of query: 206
Effective length of database: 209
Effective search space:    43054
Effective search space used:    43054
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory