Alignments for a candidate for PS417_11890 in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for PS417_11890 in Pseudomonas fluorescens GW456-L13

Align m-Inositol ABC transporter, ATPase component (itaA) (characterized)
to candidate PfGW456L13_7 ABC transporter ATP-binding protein

Query= reanno::pseudo3_N2E3:AO353_21385
         (521 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_7
          Length = 521

 Score =  257 bits (657), Expect = 6e-73
 Identities = 162/491 (32%), Positives = 254/491 (51%), Gaps = 15/491 (3%)

Query: 17  PNAIPVDEPY-LLEIINVSKGFPGVVALSDVQLRVRPGSVLALMGENGAGKSTLMKIIAG 75
           PN  P+ +P   L++  ++K +PG +A   + L + PG + AL+GENGAGKSTLMKII G
Sbjct: 2   PNHAPIPDPVPRLQLRRITKRYPGCLANDAIDLTIAPGEIHALLGENGAGKSTLMKIIYG 61

Query: 76  IYQPDAGELRLRGKPVTFDTPLAALQAGIAMIHQELNLMPHMSIAENIWIGREQLNGFHM 135
           +   D+GE+  +G+ V+   P  A   GI M+ Q  +L   +S+A+NI +      G   
Sbjct: 62  VTHADSGEVIWQGQRVSLRNPAQARGLGIGMVFQHFSLFETLSVAQNIALAMGAAAG--- 118

Query: 136 IDHREMHRCTAQLLERLRINLDPEEQVGNLSIAERQMVEIAKAVSYDSDILIMDEPTSAI 195
              +++     ++  R  + L+PE  V +LSI ERQ VEI + +  D  +LI+DEPTS +
Sbjct: 119 -TPKQLEPKIREVSRRYGMTLEPERLVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVL 177

Query: 196 TDKEVAHLFSIIADLKAQGKGIIYITHKMNEVFSIADEVAVFRDGAYIGLQRADSMDGDS 255
           T +E   LF  +  L A+G  I++I+HK+ EV ++     V R G   G           
Sbjct: 178 TPQEADDLFVTLRRLAAEGCSILFISHKLGEVRALCHSATVLRGGRVAGHCVPAECSDQQ 237

Query: 256 LISMMVGRELSQLFPVREKPIGDLLMSVRDLRLDGV------FKGVSFDLHAGEILGIAG 309
           L  +MVG     +    +   GD  + VR L              +  ++  GEI+GIAG
Sbjct: 238 LARLMVGEAAELIADYPKVTGGDACLDVRGLSWHNPDPFGCSLANIDLEVRRGEIVGIAG 297

Query: 310 LMGSGRTNVAEAIFG---ITPSDGGEICLDGQPVRISDPHMAIEKGFALLTEDRKLSGLF 366
           + G+G+  +   + G   +  +    I    +PV    P    + G A +  +R   G  
Sbjct: 298 VAGNGQDELLALLSGEALLPRNASATIRFGKEPVAHLRPDARRQLGLAFVPAERLGHGAV 357

Query: 367 PCLSVLENMEMAVLPH-YAGNGFIQQKALRALCEDMCKKLRVKTPSLEQCIDTLSGGNQQ 425
           P LS+ +N  +    H    NG IQ+  + AL E++ ++  VKTP  +    +LSGGN Q
Sbjct: 358 PELSLADNALLTAFQHGLVSNGLIQRGKVEALAEEIIRRFGVKTPDSQAPARSLSGGNLQ 417

Query: 426 KALLARWLMTNPRILILDEPTRGIDVGAKAEIYRLISYLASEGMAVIMISSELPEVLGMS 485
           K +L R ++  PR+L+   PT G+DVGA A I+R +  L   G A+++IS +L E+  +S
Sbjct: 418 KFILGREILQQPRLLVAAHPTWGVDVGAAATIHRALIALRDAGAAILVISEDLDELFQIS 477

Query: 486 DRVMVMHEGDL 496
           DR+  +  G L
Sbjct: 478 DRLGALCGGRL 488



 Score = 87.4 bits (215), Expect = 1e-21
 Identities = 60/222 (27%), Positives = 113/222 (50%), Gaps = 9/222 (4%)

Query: 295 VSFDLHAGEILGIAGLMGSGRTNVAEAIFGITPSDGGEICLDGQPVRISDPHMAIEKGFA 354
           +   +  GEI  + G  G+G++ + + I+G+T +D GE+   GQ V + +P  A   G  
Sbjct: 32  IDLTIAPGEIHALLGENGAGKSTLMKIIYGVTHADSGEVIWQGQRVSLRNPAQARGLGIG 91

Query: 355 LLTEDRKLSGLFPCLSVLENMEMAVLPHYAGNGFIQQKALRALCEDMCKKLRVKTPSLEQ 414
           ++ +      LF  LSV +N+ +A+     G      K L     ++ ++  + T   E+
Sbjct: 92  MVFQH---FSLFETLSVAQNIALAM-----GAAAGTPKQLEPKIREVSRRYGM-TLEPER 142

Query: 415 CIDTLSGGNQQKALLARWLMTNPRILILDEPTRGIDVGAKAEIYRLISYLASEGMAVIMI 474
            + +LS G +Q+  + R LM + R+LILDEPT  +      +++  +  LA+EG +++ I
Sbjct: 143 LVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVLTPQEADDLFVTLRRLAAEGCSILFI 202

Query: 475 SSELPEVLGMSDRVMVMHEGDLMGTLDRSEATQERVMQLASG 516
           S +L EV  +     V+  G + G    +E + +++ +L  G
Sbjct: 203 SHKLGEVRALCHSATVLRGGRVAGHCVPAECSDQQLARLMVG 244


Lambda     K      H
   0.321    0.137    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 579
Number of extensions: 29
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 521
Length of database: 521
Length adjustment: 35
Effective length of query: 486
Effective length of database: 486
Effective search space:   236196
Effective search space used:   236196
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources