GapMind for catabolism of small carbon sources

 

Aligments for a candidate for paaF in Pseudomonas fluorescens GW456-L13

Align trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized)
to candidate PfGW456L13_2984 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17)

Query= reanno::BFirm:BPHYT_RS17335
         (258 letters)



>lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2984 Enoyl-CoA
           hydratase [valine degradation] (EC 4.2.1.17)
          Length = 257

 Score =  317 bits (812), Expect = 2e-91
 Identities = 160/256 (62%), Positives = 200/256 (78%), Gaps = 1/256 (0%)

Query: 1   MAYENILVETRGRVGLVTLNRPKALNALNDALMDELGAALREFDADDAIGAIVVTGSEKA 60
           ++YE IL+ET GRVGL+TLNRP+ALNALN  ++ EL  AL  F+AD  IG IV+TGS+KA
Sbjct: 1   VSYETILLETHGRVGLITLNRPQALNALNAQIVSELNQALDGFEADSNIGCIVLTGSKKA 60

Query: 61  FAAGADIGMMSTYTYMDVYKGDYITRNWETVRSIRKPIIAAVAGFALGGGCELAMMCDII 120
           FAAGADI  M+  TY  +Y  D  + + + V + RKPIIAAV GFALGGGCELA+MCD I
Sbjct: 61  FAAGADIKEMAELTYPQIYIDDLFSDS-DRVANRRKPIIAAVNGFALGGGCELALMCDFI 119

Query: 121 FAADTAKFGQPEIKLGIMPGAGGTQRLPRAVSKAKAMDLCLTARFMDAAEAERAGLVSRV 180
            A D A+FGQPEI LG++PG GGTQRL RAV KAKAM++CL+ R +DA EAER G+V+R+
Sbjct: 120 LAGDNARFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCLSGRLIDAVEAERCGIVARI 179

Query: 181 IPAASLVDEAIAAAATIAEFPSPAVMMVKESVNRAYETTLAEGVHFERRLFHSLFATEDQ 240
           +P+  L+DEA+  AA IA+   P  MMVKESVNRA+E +LAEGV FERR+FH+ FAT+DQ
Sbjct: 180 VPSDELLDEALKVAALIAKKSLPIAMMVKESVNRAFEVSLAEGVRFERRVFHAAFATQDQ 239

Query: 241 KEGMAAFVEKRKPVFK 256
           KEGMAAF+ KR+  F+
Sbjct: 240 KEGMAAFIAKREAEFQ 255


Lambda     K      H
   0.321    0.134    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 217
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 258
Length of database: 257
Length adjustment: 24
Effective length of query: 234
Effective length of database: 233
Effective search space:    54522
Effective search space used:    54522
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory