Alignments for a candidate for pimF in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Pseudomonas fluorescens GW456-L13

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate PfGW456L13_4041 Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

Query= metacyc::MONOMER-20678
         (699 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4041
          Length = 715

 Score =  340 bits (871), Expect = 2e-97
 Identities = 224/692 (32%), Positives = 352/692 (50%), Gaps = 33/692 (4%)

Query: 23  VNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGADITEFGKPPQPP------- 75
           VN  +      + + V+   AD +++ +++      FI GADITEF    + P       
Sbjct: 29  VNKFNRLTLNELRQAVDTIKADASIKGVIVSSGKDVFIVGADITEFVDNFKLPDAELVAG 88

Query: 76  --ALNDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVKEAKLGLPEVKLGLLPG 133
               N + +  E+   PT+AAI+G ALGGGLE+ L   +RV   +AK+GLPEVKLG+ PG
Sbjct: 89  NLEANKIFSDFEDLNVPTVAAINGIALGGGLEMCLAADYRVMSTKAKIGLPEVKLGIYPG 148

Query: 134 AGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVV--ENLVAGAVAFAKKVLA- 190
            GGT RLPR +G + A++ I  G      +ALK G V+ VV  E L   A+   K+ ++ 
Sbjct: 149 FGGTVRLPRLIGADNAIEWIAAGKENRPEDALKVGAVDAVVAPEQLQDAALELIKRAISG 208

Query: 191 --EKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMTKKARGLEAPFACADAIGAAIDLPFE 248
             + +  R+ + +  KL A +   +  T       +      AP      I  A +   +
Sbjct: 209 EFDYKAKRQPKLEKLKLNAIEQMMAFETAKGFVAGQAGPNYPAPVEAIKTIQKAANFGRD 268

Query: 249 EGLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGTKPRPVSRVAIIGAGTMGG 308
           + L+ E  GF+KL  +  +++    F  ++E  K     D    + V + A++GAG MGG
Sbjct: 269 KALEIEAAGFVKLAKTSAAQSLIGLFLNDQELKKKAKAYDEIA-KDVKQAAVLGAGIMGG 327

Query: 309 GIAMSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARGGLPPDAPAKRMALITGLVG 368
           GIA   A+ G P+ + +  E  +++GL    K       +G +     A+ +  I   + 
Sbjct: 328 GIAYQSASKGTPILMKDINEHGIEQGLAEAAKLLVGRVDKGRMTAAKMAEVLNGIRPTLS 387

Query: 369 LENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASNTSYLSIDEIAATTKRPQDV 428
             +    DL++EAV E   VK+ V   V+   K   +LASNTS +SI  +A   KRP++ 
Sbjct: 388 YGDFGHVDLVVEAVVENPKVKQAVLAEVEDKVKEDTILASNTSTISITLLAKALKRPENF 447

Query: 429 LGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKVPVVVGVCDGFVGNRMLAAR 488
           +GMHFF+P ++M L E++RG K++  A+ T V+ AKK+ K P+VV  C GF+ NR+L   
Sbjct: 448 VGMHFFNPVHMMPLVEVIRGEKSSELAVATTVAYAKKMGKNPIVVNDCPGFLVNRVLFPY 507

Query: 489 SKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIGWRSR---------KDRGI 539
                KL+  G    ++D ++ KFG PMGP  + D+ G+D G   R         + +  
Sbjct: 508 FGGFAKLVSAGVDFVRIDKIMEKFGWPMGPAYLMDVVGIDTGHHGRDVMAEGFPDRMKDD 567

Query: 540 KSEIADALCEAGRFGQKTGKGYYKYE---QGSRAPMPDPEVETLINDTLAKLGLKRRDIT 596
           +    D L EA R GQK GKG+Y YE   +G +  + DP V     + L  +  ++R++T
Sbjct: 568 RRSAVDVLYEAKRLGQKNGKGFYAYETDKKGKQKKVADPSV----LEVLKPIVYEQREVT 623

Query: 597 DEEILERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIYRGGPMHYADSVGLKHI 656
           DE+I+  M+ P+  E  R LE+ I    ++ D+  +YG G+P +RGG + Y DS+G+   
Sbjct: 624 DEDIINWMMIPLCLETVRCLEDGIVETAAEADMGLVYGIGFPPFRGGALRYIDSIGVAEF 683

Query: 657 AERLSAYAKATNDPSLEPAPLLARLAAEGKTF 688
                 YA         P   L  +A  G++F
Sbjct: 684 VALADQYADL--GALYHPTAKLREMAKNGQSF 713


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1006
Number of extensions: 64
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 715
Length adjustment: 39
Effective length of query: 660
Effective length of database: 676
Effective search space:   446160
Effective search space used:   446160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources