Alignments for a candidate for ppa in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for ppa in Pseudomonas fluorescens GW456-L13

Align Phenylacetate permease, Ppa (characterized)
to candidate PfGW456L13_2421 Acetate permease ActP (cation/acetate symporter)

Query= TCDB::O50471
         (520 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2421
          Length = 548

 Score =  915 bits (2364), Expect = 0.0
 Identities = 463/520 (89%), Positives = 496/520 (95%)

Query: 1   MNWTAISMFMVFVCFTLLVTRWAALRTRSASDFYTAGGGLTGMQNGLAIAGDMISAASFL 60
           +NW AI MF+VFV FTL VTRWAALRTRS SDFYTAGGG+TG QNGLAIAGDMISAASFL
Sbjct: 29  LNWNAIGMFLVFVLFTLGVTRWAALRTRSTSDFYTAGGGMTGFQNGLAIAGDMISAASFL 88

Query: 61  GISAMMFMNGYDGLLYALGVLAGWPIILFLIAERLRNLGKYTFADVVSYRLAQTPVRLTS 120
           GISAMMF+NGYDGLLYALGVLAGWPIILFLIAERLRNLGKYTFADVVSYRL QTPVRLTS
Sbjct: 89  GISAMMFLNGYDGLLYALGVLAGWPIILFLIAERLRNLGKYTFADVVSYRLEQTPVRLTS 148

Query: 121 AFGTLVVALMYLVAQMVGAGKLIELLFGISYLYAVMLVGVLMVAYVTFGGMLATTWVQII 180
           AFGTL VALMYLVAQMVGAGKLIELLFGI YLYAVMLVGVLMV YVTFGGMLATTWVQII
Sbjct: 149 AFGTLTVALMYLVAQMVGAGKLIELLFGIDYLYAVMLVGVLMVFYVTFGGMLATTWVQII 208

Query: 181 KAVMLLSGTSFMAFMVLKHFGFSTEAMFASAVAVHAKGQAIMAPGGLLSNPVDAISLGLG 240
           KAVMLL GTSFMAFMVLKHFGFSTEAMFA A AVHAKG AIMAPGGLLSNP+DAISLGLG
Sbjct: 209 KAVMLLFGTSFMAFMVLKHFGFSTEAMFAGATAVHAKGNAIMAPGGLLSNPIDAISLGLG 268

Query: 241 MMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYLLLIVIGFGAIVMVGTEPSYR 300
           MMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYLLLI++GFGAIVMVGT+P++R
Sbjct: 269 MMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYLLLIIVGFGAIVMVGTDPAFR 328

Query: 301 DATGAIIGGGNMIAVHLAQAVGGNLFLGFISAVAFATILAVVAGLALSGASAVSHDLYAC 360
           DA+GAIIGGGNM+AVHLA AVGGNLFLGFISAVAFATILAVVAGLALSGASAVSHDLYAC
Sbjct: 329 DASGAIIGGGNMVAVHLAHAVGGNLFLGFISAVAFATILAVVAGLALSGASAVSHDLYAC 388

Query: 361 VIRQGKATEQEEMRVSRIATLLIGLLAVLLGLMFESQNIAFLSGLVLAVAASVNFPVLLL 420
           V+R+G+A+EQ+EMRVSRIATL IG+LA++LGL+FESQNIAFLSGLVLA+AASVNFPVL L
Sbjct: 389 VMRKGQASEQQEMRVSRIATLCIGVLAIVLGLLFESQNIAFLSGLVLAIAASVNFPVLFL 448

Query: 421 SMFWKGLTTRGAVCGSMAGLASAVLLVVLGPAVWVNVLHHEKALFPYSNPALFSMSLAFL 480
           SM+WKGLTTRGAV GS++GL SA++L+VL PAVWVNV+HH+KALFPYSNPALFSMSLAF 
Sbjct: 449 SMYWKGLTTRGAVLGSLSGLVSAIVLLVLSPAVWVNVMHHDKALFPYSNPALFSMSLAFF 508

Query: 481 SAWVFSVTDSSERASEERGRYLAQFIRSMTGIGAAGASKH 520
           SAW+FSVTD+S RA+ ERGRYLAQFIRSMTGIGA GASKH
Sbjct: 509 SAWLFSVTDTSPRAALERGRYLAQFIRSMTGIGATGASKH 548


Lambda     K      H
   0.328    0.139    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1075
Number of extensions: 29
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 548
Length adjustment: 35
Effective length of query: 485
Effective length of database: 513
Effective search space:   248805
Effective search space used:   248805
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources