Align ring 1,2-phenylacetyl-CoA epoxidase PaaC subunit (EC 1.14.13.149) (characterized)
to candidate PfGW456L13_2425 Phenylacetate-CoA oxygenase, PaaI subunit
Query= metacyc::MONOMER-15949 (253 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2425 Length = 253 Score = 412 bits (1060), Expect = e-120 Identities = 197/253 (77%), Positives = 221/253 (87%) Query: 1 MNPNHDLIEYLLRLGDSALIQGQRLCEWCGRAPALEEELALMNVGLDLVGQARNWLDYAA 60 MN DLIEYLLRLGDSALIQGQRLC+WCG+APALEEELALMNVGLDLVGQARNWL+YAA Sbjct: 1 MNNKTDLIEYLLRLGDSALIQGQRLCQWCGKAPALEEELALMNVGLDLVGQARNWLEYAA 60 Query: 61 ELLADGRDADHLAFRRDERAYRNLLLVEQPNGDFAVTMAKQFLYDAWHFQVLDGLSRSGD 120 ELL DGRDADHLAFRRDERAYRNLLLVEQPNGDFA+TM KQFLYDAWH VL GLS+S D Sbjct: 61 ELLDDGRDADHLAFRRDERAYRNLLLVEQPNGDFAITMLKQFLYDAWHLPVLQGLSQSSD 120 Query: 121 ARVAGIAAKALKEVTYHLRRSGEWVQRLGDGTEESHRRMQAAIPQLWRFTVEMSDGDEVE 180 R+AGIAAKA+KEVTYHLRRSGEW++R+GDGTEES+RRM AAIP++WRFTVE+ D+ E Sbjct: 121 ERIAGIAAKAVKEVTYHLRRSGEWIERMGDGTEESNRRMLAAIPEVWRFTVELIAADDSE 180 Query: 181 QRLCEAGIAPDPAQIAGAWQAKVAEVFAAATLPLPEPAVNFYLSGRRGLHSEHLGLLLAE 240 QRLC AGI PD A +A WQ KV ++F +ATLP+P FYL+ R+GLH+EHLG+LLAE Sbjct: 181 QRLCAAGITPDIASVATQWQTKVEQIFTSATLPVPAAPSYFYLNARKGLHTEHLGILLAE 240 Query: 241 MQFLQRAYPDATW 253 MQ L RAYPDATW Sbjct: 241 MQCLPRAYPDATW 253 Lambda K H 0.321 0.136 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 302 Number of extensions: 3 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 253 Length of database: 253 Length adjustment: 24 Effective length of query: 229 Effective length of database: 229 Effective search space: 52441 Effective search space used: 52441 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory