Alignments for a candidate for pad-dh in Pseudomonas fluorescens GW456-L13

GapMind for catabolism of small carbon sources

Alignments for a candidate for pad-dh in Pseudomonas fluorescens GW456-L13

Align phenylacetaldehyde dehydrogenase monomer (EC 1.2.1.39) (characterized)
to candidate PfGW456L13_2360 Aldehyde dehydrogenase (EC 1.2.1.3)

Query= metacyc::MONOMER-15732
         (497 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2360
          Length = 496

 Score =  822 bits (2124), Expect = 0.0
 Identities = 407/491 (82%), Positives = 443/491 (90%)

Query: 7   ILPATRAFLERKLKMRIGADWQDAASGRTLSFRNPATGEVLGEVPAADAEDVDRAVRAAR 66
           +LPAT AF++R  +M IG DW +AA G+T+   NPATGEVL  VP A  EDVDRAV AAR
Sbjct: 6   LLPATSAFIQRAPRMLIGGDWVEAADGQTMPLHNPATGEVLCVVPRATPEDVDRAVLAAR 65

Query: 67  QAFDDSPWSRLRPRERQNLLWRLADLMERDARQLAELECLNNGKSAAVAQVMDVQLAIDF 126
           QAFDDS W+R RPRERQNLLW+LADLM+RDA  LA+LECLNNGKSAAVAQVMDVQLAIDF
Sbjct: 66  QAFDDSAWTRTRPRERQNLLWKLADLMQRDAELLAQLECLNNGKSAAVAQVMDVQLAIDF 125

Query: 127 LRYMAGWATKIEGSTVEASMPLMPNDQFHGFVRREAIGVVGAIVAWNFPLLLACWKLGPA 186
           LRYMAGWATKIEGSTV+ S PLMPNDQFH F+RREA+GVVGAIVAWNFPLLLACWKLGPA
Sbjct: 126 LRYMAGWATKIEGSTVDVSAPLMPNDQFHSFIRREAVGVVGAIVAWNFPLLLACWKLGPA 185

Query: 187 LATGCTIVLKPADETPLSVLKLAELVDEAGYPAGVFNVVTGTGLNAGAALSRHPGVDKLT 246
           LATGCT+VLKPADETPL+ LKLAELV EAGYP GVFNVVTGTG+ AG+AL+ +P VDKLT
Sbjct: 186 LATGCTVVLKPADETPLTALKLAELVLEAGYPEGVFNVVTGTGITAGSALTHNPLVDKLT 245

Query: 247 FTGSTEVGKLIGKAAMDNMTRVTLELGGKSPTIVMPDANLQEAAAGAATAIFFNQGQVCC 306
           FTGST VGK IGK AMD+MTRVTLELGGKSPTIVM DA+L+ AAAGAA+AIFFNQGQVCC
Sbjct: 246 FTGSTAVGKQIGKIAMDSMTRVTLELGGKSPTIVMADADLKTAAAGAASAIFFNQGQVCC 305

Query: 307 AGSRLYVHRKHFDNVVADIAGIANGMKLGNGLDPAVQMGPLISAKQQDRVTGYIELGREL 366
           AGSRLYV RKHFDNVVADI+ IAN MKLGNGLDP+V MGPLISA+QQ+RV GYIE GRE 
Sbjct: 306 AGSRLYVQRKHFDNVVADISDIANAMKLGNGLDPSVDMGPLISARQQERVYGYIEKGRES 365

Query: 367 GATVACGGEGFGPGYFVKPTVIVDVDQRHRLVQEEIFGPVLVAMPFDDLDEVIGMANDNP 426
           GAT+ACGGE FGPG+FVKPTVIVDVDQ+H LVQEEIFGPVLVA+PFDD  + + MAND+P
Sbjct: 366 GATIACGGEQFGPGFFVKPTVIVDVDQKHSLVQEEIFGPVLVAIPFDDEADALRMANDSP 425

Query: 427 YGLGASIWSNDLAAVHRMIPRIKSGSVWVNCHSALDPALPFGGYKMSGVGREVGAAAIEH 486
           YGLGASIWSNDLAAVHRMIPRIKSGSVWVNCHSALDPALPFGGYKMSGVGRE+G AAIEH
Sbjct: 426 YGLGASIWSNDLAAVHRMIPRIKSGSVWVNCHSALDPALPFGGYKMSGVGREMGYAAIEH 485

Query: 487 YTELKSVLIKL 497
           YTELKSVLIKL
Sbjct: 486 YTELKSVLIKL 496


Lambda     K      H
   0.320    0.137    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 901
Number of extensions: 27
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 497
Length of database: 496
Length adjustment: 34
Effective length of query: 463
Effective length of database: 462
Effective search space:   213906
Effective search space used:   213906
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources