GapMind for catabolism of small carbon sources

 

Finding step aapP for L-proline catabolism in Pseudomonas fluorescens GW456-L13

4 candidates for aapP: ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), ATPase component AapP

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
med PfGW456L13_1701 ABC-type polar amino acid transport system, ATPase component AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 77% 100% 415.6 ABC transporter for D-Alanine, ATPase component 99% 511.1
med PfGW456L13_4773 Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4) AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 60% 93% 291.2 ABC transporter for L-Asparagine and possibly other L-amino acids, putative ATPase component 100% 488.0
med PfGW456L13_376 Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1) AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 52% 93% 241.9 ABC transporter for L-Lysine, ATPase component 97% 487.6
med PfGW456L13_446 Cystine ABC transporter, ATP-binding protein AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 52% 93% 236.9 L-cystine transport system ATP-binding protein YecC; EC 7.4.2.- 76% 366.3

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step aapP

Or cluster all characterized aapP proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory